Epstein-Barr virus (EBV), is the herpesvirus that causes infectious mononucleosis and is associated with a number of cancers, particularly lymphomas in immunosuppressed persons including persons with AIDS EBV is a ubiquitous virus, so common that it has been difficult to determine whether it is the cause of certain diseases or whether it is simply there as an artifact

Images removed due to copyright considerations. See the following: Figure 1 and Table 1 in Reed SI. 2003. Ratchets and clocks: The cell cycle nover ubiquitylation and protein turnover. Nat Rev. Mol. Cell Biol. 4: 855-864. Figure 1 in Bartek J, Lukas J. Mammalian G1-and S-phase checkpoints in response to DNA damage. Curr Opin Cell

How p53 and Rb pathway function may be disrupted in cancer cells Components of the pathway which are found altered in human cancers are shown in red on the diagram above p53 and Rb themselves may be inactivated by gene mutation (loss of both copies or also as in familial retinoblastoma and Li-Fraumeni syndrome where there are inherited mutations in one copy of Rb or p53 gene respectively. Alternatively

DISCOVERy OF THE ROLE OF UBIQUITIN IN PROTEIN DEGRADATION HISTORICAL FACTS Courtesy of Sam Griffiths-Jones. Used with permission Source: \Peptide models for protein beta-sheets hD thesis, University of Nottingham, 2001 8(1975)Ubiquitin was first isolated by Gideon Goldstein and colleagues from the thymus(reason why it was originally thought to be a thymic hormone)

第一节氨基酸 一、分类、命名和构型 1.分类:按烃基类型可分为脂肪族氨基酸,芳香族氨基酸,含杂环氨 基酸。 按分子中氨基和羧基的数目分为中性氨基酸,酸性氨基酸, 碱性氨基酸

物质在生物体内进行氧化称生物氧化,主要指糖、脂肪、蛋白质等在体内分解时逐步释放能量,最终生成CO2和H2O的过程

物质在生物体内进行氧化称生物氧化，主要指糖、脂肪、蛋白质等在体内分解时逐步释放能量，最终生成CO2和 H2O的过程

一、化学交换现象 1 一个核在A,B两个不同状态下的交换 2 A,B两个不同状态: (1)处于不同分子, 如蛋白质上的NH,OH质子与水中质子交换 (2)同一个分子的不同构象 如顺式与反式异构体

利用某些物质的分子吸收紫外光、可见光 、红外光和激光200800nm光谱区的辐射来 进行定性定量分析和物质结构分析测定的方法 。称为分光光度法或分光光度技术,使用的仪 器称为分光光度计 这种分光光度计灵敏度高,测定速度快 ,应用范围广,其中的紫外/可见分光光度技术 更是生物化学研究工作中必不可少的基本手段 之一

第一节 预处理（细胞破碎方法及其原理） 第二节 酶的提取 第三节 酶的分离纯化（沉淀分离） 第四节 酶的分离纯化（离心分离） 第三节 酶的分离纯化（过滤与膜分离）