1. Please describe the main features of the DNA structure. (10 points) Key features: (1) Double helical structure (4’) (2) Two strands are held together in an antiparallel orientation (3’) by base paring (1’)
Part I: Describe the following 10 terms (3 points each). (1) EF-G: a. its structure likes tRNA b. it is a translational elongation factor c. it binds to ribosome when associated with GTP
Q1: Please name the following amino acids (three letter name, for example: Ala for alanine). Q2: Which of them are hydrophobic AA, and which are hydrophilic?
1. What is “DNA cloning”? how could you make a genomic library cloning? (30 points) 2. What is “subcloning”? What are the purposes of subcloning. (15 points)