生物化学是一门研究生物体内化学分子与化学反应的学科。是在分子水平上研究生物体的分子结构和功能、物质代谢与调节，以及遗传信息传递的分子基础与调控规律。 第一节 蛋白质的分子组成 第二节 蛋白质的分子结构 第三节 蛋白质结构与功能的关系 第四节 蛋白质的理化性质 第五节 蛋白质的分离、纯化

DNA克隆(DNA cloning)：应用酶学的方法，在 体外将目的基因与载体DNA结合成一具有自我复 制能力的DNA分子—复制子，继而通过转化或转 染宿主细胞、筛选出含有目的基因的转化子细胞， 再进行扩增、提取获得大量同一DNA分子的过程。 DNA克隆又称基因克隆（gene cloning）

一、翻译（translation）：mRNA分子中的遗传信息转变为蛋白质的氨基酸排列顺序 二、基因表达（Gene expression）：转录与翻译

1 、共有的代谢池 2、动态平衡，以防止中间产物的堆积和缺乏 4、代谢调节与协调 5、组织、器官的代谢各有特色，相互配合形成整体 6、ATP是机体能量利用的共同形式

“细胞周期”（cell cycle）又称“细胞分裂 周期” ，是指一个细胞经生长、分裂形成两 个细胞所经历的全过程，即细胞从一次分裂 结束到下一次分裂结束所经历的全过程

Part I Molecular Components of Cells 1 The Facts of Life: Chemistry Is the Logic of Biological Phenomena 2 Water: The Medium of Life 3 Thermodynamics of Biological Systems 4 Amino Acids and the Peptide Bond 5 Proteins: Their Primary Structure and Biological Functions 6 Proteins: Secondary, Tertiary, and Quaternary Structure 7 Carbohydrates and the Glycoconjugates of Cell Surfaces 8 Lipids 9 Membranes and Membrane Transport 10 Nucleotides and Nucleic Acids 11 Structure of Nucleic Acids 12 Recombinant DNA, Cloning, Chimeric Genes, and Synthetic Biology Part II Protein Dynamics 13 Enzymes—Kinetics and Specificity 14 Mechanisms of Enzyme Action 15 Enzyme Regulation 16 Molecular Motors Part III Metabolism and Its Regulation Part IV Information Transfer

Chapter 2 Water Chapter 3 Amino Acids, Peptides, and Proteins Chapter 4 The Three-Dimensional Structure of Proteins Chapter 5 Protein Function Chapter 6 Enzymes Chapter 8 Nucleotides and Nucleic Acids Chapter 9 DNA-Based Information Technologies Chapter 11 Biological Membranes and Transport Chapter 12 Biosignaling Chapter 13 Bioenergetics and Biochemical Reaction Types Chapter 14 Glycolysis, Gluconeogenesis, and the Pentose Chapter 16 The Citric Acid Cycle Chapter 17 Fatty Acid Catabolism Chapter 18 Amino Acid Oxidation and the Production of Urea Chapter 19 Oxidative Phosphorylation and Photophosphorylation Chapter 20 Carbohydrate Biosynthesis in Plants and Bacteria Chapter 22 Biosynthesis of Amino Acids, Nucleotides, and Related Molecules Chapter 24 Genes and Chromosomes Chapter 25 DNA Metabolism Chapter 26 RNA Metabolism Chapter 28 Regulation of Gene Expression

UNIT I: Protein Structure and Function Chapter 1: Amino Acids Chapter 2: Structure of Proteins Chapter 3: Globular Proteins Chapter 4: Fibrous Proteins Chapter 5: Enzymes UNIT II: Intermediary Metabolism Chapter 6: Bioenergetics and Oxidative Phosphorylation Chapter 7: Introduction to Carbohydrates Chapter 8: Glycolysis Chapter 9: Tricarboxylic Acid Cycle Chapter 10: Gluconeogenesis Chapter 11: Glycogen Metabolism Chapter 12: Metabolism of Monosaccharides and Disaccharides Chapter 13: Pentose Phosphate Pathway and NADPH Chapter 14: Glycosaminoglycans, Proteoglycans, and Glycoproteins UNIT III: Lipid Metabolism Chapter 15: Metabolism of Dietary Lipids Chapter 16: Fatty Acid and Triacylglycerol Metabolism Chapter 17: Complex Lipid Metabolism Chapter 18: Cholesterol and Steroid Metabolism UNIT IV: Nitrogen Metabolism Chapter 19: Amino Acids: Disposal of Nitrogen Chapter 20: Amino Acid Degradation and Synthesis Chapter 21: Conversion of Amino Acids to Specialized Products Chapter 22: Nucle

一、基因治疗的策略 The Strategy of Gene Therapy 二、基因转移技术 The Technique of Gene Transfer 三、基因干预 Gene Interference 四、治疗基因的受控表达 Regulated Expression of Therapeutic Gene 五、基因治疗的应用研究 The Application of Gene Therapy

核酸是以核苷酸为基本组成单位的生物大分子，携带和传递遗传信息。 第一节 核酸的化学组成及一级结构 第二节 DNA的空间结构与功能 第三节 RNA的结构与功能 第四节 核酸的理化性质 一、一般理化性质 二、DNA变性 三、 DNA的复性与分子杂交 第五节 核酸酶