UNIT CELL PROCESSES Regulator UCP Cell Matrix Product Regulator Connect. ECM Mitosis Tiss. Adhesion Sy Synthesis Epithelia |Protein Migration Muscle Collagen

一、 DNA水平的调控 二、 转录水平的调控(transcriptional regulation) 三、转录后水平的调控(post transcriptionalregulation) 四、翻译水平的调控(translational regulation) 五、 翻译后水平的调控(protein maturation)

Types of chemotaxins – C5a attracts neutrophils and monocytes – Made by bacteria Peptide clipped off N-terminus (beginning with N￾formylmethionine) during peptide maturation after protein synthesis

E1.Membrane lipids E2.Membrane protein and carbohydrate E3.Membrane transport:small molecules E4.Membrane transport:macromolecules E5.Signal transduction

一、氨基酸 (Amino Acid)（重点内容） （一）结构特点 （二）20 种基本氨基酸 （三）氨基酸的特性 （四）氨基酸的合成与拆分 二、肽 (Peptides) 三、蛋白质 (Proteins)（简述）

Bacterial chemical components Water: free water and compound water. Inorganic salt: phosphus, potassium magnesium,calcium, nitrium,etc. Protein: 50%-80% of dry weight accordingbacterial kinds and age. Sugar: mainly distributing in cell wall andcapsule. Lipids: composed of lipid, fatty acid, wax, etc. Nucleic acid: RNA and DNA

Chapter 1: Plasmids and Their Usefulness in Molecular Cloning Chapter 2: Bacteriophage and Its Vectors Chapter 3: Working with Bacteriophage M13 Vectors Chapter 4: Working with High-Capacity Vectors Chapter 5: Gel Electrophoresis of DNA and Pulsed-Field Agarose Chapter 6: Preparation and Analysis of Eukaryotic Genomic DNA Chapter 7: Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells Chapter 8: In Vitro Amplification of DNA by the Polymerase Chain Reaction Chapter 9: Preparation of Radiolabeled DNA and RNA Probes Chapter 10: Working with Synthetic Oligonucleotide Probes Chapter 11: Preparation of cDNA Libraries and Gene Identification Chapter 12: DNA Sequencing Chapter 13: Mutagenesis Chapter 14: Screening Expression Libraries Chapter 15: Expression of Cloned Genes in Escherichia coli Chapter 16: Introducing Cloned Genes into Cultured Mammalian Cells Chapter 17: Analysis of Gene Expression in Cultured Mammalian Cells Chapter 18: Protein Interaction Technologies

The nucleic acids, deoxyribonucleic (DNA) and ribonucleic (RNA) , are, respectively , the molecules that preserve here￾ditary information and that transcribe and translate it in a way that allows the synthesis of all the varied proteins of the cell 25.2 NUCLEOTIDES AND NUCLEOSIDES

复旦大学：《生物化学A Biochemistry》课程PPT教学课件（上）04 蛋白质化学 Protein Chemistry（二）蛋白质的一级结构及其分析

复旦大学：《生物化学A Biochemistry》课程PPT教学课件（上）06 蛋白质化学 Protein Chemistry（四）蛋白质的高级结构（超二级结构和三、四级结构）