• 癌基因和抑癌基因 • 癌基因和抑癌基因在细胞增殖调控中的 作用 • 癌基因和抑癌基因与肿瘤发生

Electron Microscopy of replicating DNA reveals replicating bubbles. How does one prove bidirectional fork movement?

Translation in prokaryotes • 蛋白质生物合成体系 • 氨基酸的活化与搬运 • 核糖体循环 • 多聚核糖体 Translation in eukaryotes Posttranslational processing

华南师范大学：《医学分子生物学》课程教学课件（PPT讲稿）第五章 基因表达（转录）Gene Expression

基因诊断—以DNA或RNA为诊断材 料，通过检查基因的存在、缺陷或 表达异常，对人体状态和疾病作出 诊断的方法和过程

Announcements: Power Point Lecture Notes Available on BCMP200 Website For further reading Nucleic Acids: Structures, Properties, and Functions V.A. Bloomfield D. M. Crothers

Equilibria and free energies Estimate binding site occupancy Forces driving protein-DNA interactions Lambda repressor Methods to study protein-DNA interactions

Techniques to manipulate DNA Phenol/chloroform extraction Ethanol precipitation Concentration determination Gel Electrophoresis DNA hybridization Southern blotting Melting temperatures

In vivo dna binding pattern of the polycomb Txn factor 1. What are the genes towhich it binds? 2. how does it affect theserenes? 3. What determines where itBinds??

Molecular Biology of the Gene, 5/E---Watson et al. (2004) Part I: Chemistry and genetics Part l: Maintenance of the Genome Part I: Expression of the Genome Part IV: Regulation Part v: Methods