BIOMATERIALS-TISSUE INTERACTIONS: \Tools\ for Understanding the Molecular, Cellular, and Physiological, Bases of the Tissue Response to Implants M. Spector, Ph. D. and I. V. Yannas, Ph.D. BIOMATERIALS-TISSUE INTERACTIONS

Outline of three lectures on Irreversible Healing of Extracellular Matrix. A. Irreversible healing of ECMs in different organs. B. Structure and function of naturally occurring ECMs. C. Synthesis of biologically active ECM models

Introduction. Chapter 1. Irreversible Healing of Extracellular Matrix. Chapter 2. Cell-Matrix Interactions. Chapter 3. Synthesis of Tissues and Organs. TEXT: I. V. Yannas. Tissue and Organ Regeneration in Adults. New York: Springer, 2001

UNIT CELL PROCESSES Regulator UCP Cell Matrix Product Regulator Connect. ECM Mitosis Tiss. Adhesion Sy Synthesis Epithelia |Protein Migration Muscle Collagen

RESPONSE TO IMPLANTS WOUND HEALING flury Vascular Response Inflammation Tissue of labile Tissue of and stable cells Permanent cells Framework framework Scar Intact Destroyed

RESPONSE TO IMPLANTS: WOUND HEALING Surgical Implantation Inc. time Vascular Response Acute Clotting Inflammation Phagocytosis Granulation Neovascularization New Collagen Synthesis Tissue

The Extracellular Matrices Part. 2. Elastin fibers. 3. Proteoglycans(PG) and glycosaminoglycans (GAG). 4. Cell-adhesion molecules (CAM)

TISSUE ENGINEERING What is tissue engineering Production of tissue in vitro by growing cells in porous, absorbable scaffolds (matrices). Why is tissue engineering necessary? Most tissues cannot regenerate when injured or diseased

UNIT CELL PROCESSES Concept of a \Control Volume\ around a Cell Soluble Regulator Cell Matrix

C. Cell-Matrix Interactions A. How cells pull onto and deform the matrix to which they attach themselves. B. Cell-matrix interactions control the spontaneous closure of wounds in organs. C. What happens when regeneration is induced?