新视野系列研究生课程:科技论文的构思、撰写和发表 生物医学世纪讲坛 How to Publish your papers in the top scientific journals 教授
新视野系列研究生课程：科技论文的构思、撰写和发表 生物医学世纪讲坛 How to Publish Your Papers in the Top Scientific Journals 鲁白教授
鲁白教授 1957年12月生于上海市。1982年获上海华东师范大学学士学 位。1990年获康乃尔大学博士学位后,在洛克菲勒大学和哥伦 比亚大学从事博士后研究,导师为 Paul Greengard和蒲慕明教授。 1993年加入罗氏分子生物学研究所,并任哥伦比亚大学助理教 授。19%6年加入NH儿童健康和人类发育研究所,任突触发育和 可塑性研究室主任。 主要研究神经营养因子在突触发育和可塑性中的作用。他 的硏究室是最早提出并发现神经营养因子对神经系统突触传递、 突触发育可塑性有调控作用的实验室之一,与几个著名实验室 一起开创了神经营养因子的突触调控这一新领域
鲁白教授 1957年12月生于上海市。1982年获上海华东师范大学学士学 位。1990年获康乃尔大学博士学位后，在洛克菲勒大学和哥伦 比亚大学从事博士后研究,导师为Paul Greengard和蒲慕明教授。 1993年加入罗氏分子生物学研究所，并任哥伦比亚大学助理教 授。1996年加入NIH儿童健康和人类发育研究所，任突触发育和 可塑性研究室主任。 主要研究神经营养因子在突触发育和可塑性中的作用。他 的研究室是最早提出并发现神经营养因子对神经系统突触传递、 突触发育可塑性有调控作用的实验室之一，与几个著名实验室 一起开创了神经营养因子的突触调控这一新领域
怎样在国际权威杂志上发表科学论文 Bailu 鲁迅 Lu Xun 鲁白 李白 Li Bai (1881-1936) (701-762) F The father of modern The most influential Chinese literatures poet in Chinese history
Bai Lu 怎 样 在 国 际 权 威 杂 志 上 发 表 科 学 论 文 鲁 迅 Lu Xun 鲁 白 (1881-1936) The father of modern Chinese literatures The most influential poet in Chinese history 李 白 Li Bai (701-762)
2003 research articles 1. M. F. Egan, M. Kojima, J. H Callicott, T.E. Goldberg, B. S. Kolachana, E. Zaistev, A. Bertolino, B. Gold, D Goldman, M. Dean, B Lu,(co-corresponding author ) and D.r. Weinberger. (2003)The BDNF val66met polymorphism affects activity-dependent secretion of BDNF and human memory and hippocampal function Cell 112.257-269 L. Ma, Y.-Z. Huang, J. Valtschanoff, L. Feng, B. Lu, W. Xiong, R. Weinberg, L. Mei.(2003) Ligand dependent recruitment of the neuregulin signaling complex into neuronal lipid rafts. J. Neurosci 23, 3164 3175 3. J. Wang, C -Q. Chen, B Lu, and C -P Wu(2003 )GDNF acutely potentiates Cat* channels and excitatory synaptic transmission in midbrain dopaminergic neurons. NeuroSignals 12, 78-88 4. M. Miura, s. Gronthos, M. Zhao, B Lu, L. W. Fisher, P G robey, and s Shi(2003)SHED: Stem cells from human exfoliated deciduous teeth. Proc. Natl. Acad. Sci. USA 100, 5807-5812 5. Y.X. Zhou, M. Zhao, K Shimazu, K Sakata, D. Li, C -X. Deng, B Lu(2003)Impairments in cerebellum Purkinje cells and motor function in mice lacking Smad4 in the central nervous system. Biol. Chem. 278 42313-42320 6. J. Du, L Feng, E. Zaitsev, H.S.Je, X. Liu, and B. Lu(2003)Activity- and tyrosine kinase-dependent facilitation of TrkB receptor internalization in hippocampal neurons. J Cell Biol. In press 7. Z.G. Luo, H -SJe, F. Yang, W.C. Xiong, B. Lu, and L. Mei(2003) Activation of geranylgenany Transferase is essential for Agrin-induced AChR clustering. Neuron In press 8. S.X. Bamji, K Shimazu, N. Kimes, J. Huelsken, W. Birchmeier, B. Lu, L. F Reichardt(2003 )Regulation of presynaptic assembly and maintenance by B-catenin. Neuron In Press 9. F. Yang, X. He, J. Russell, and B. Lu (2003)Ca2* influx independent transmitter release mediated by mitochondrial Na*-Cat exchanger and protein kinase C. Cell Biol In press
1. M. F. Egan, M. Kojima, J. H. Callicott, T. E. Goldberg, B. S. Kolachana, E. Zaistev, A. Bertolino, B. Gold, D. Goldman, M. Dean, B. Lu, (co-corresponding author) and D. R. Weinberger. (2003) The BDNF val66met polymorphism affects activity-dependent secretion of BDNF and human memory and hippocampal function. Cell 112, 257-269. 2. L. Ma, Y. -Z. Huang, J. Valtschanoff, L. Feng, B. Lu, W. Xiong, R. Weinberg, L. Mei. (2003) Liganddependent recruitment of the neuregulin signaling complex into neuronal lipid rafts. J. Neurosci. 23, 3164- 3175. 3. J. Wang, C. -Q. Chen, B. Lu, and C. -P. Wu. (2003) GDNF acutely potentiates Ca2+ channels and excitatory synaptic transmission in midbrain dopaminergic neurons. NeuroSignals 12, 78-88. 4. M. Miura, S. Gronthos, M. Zhao, B. Lu, L. W. Fisher, P. G. Robey, and S. Shi (2003) SHED: Stem cells from human exfoliated deciduous teeth. Proc. Natl. Acad. Sci. USA 100, 5807-5812. 5. Y. X. Zhou, M. Zhao, K. Shimazu, K. Sakata, D. Li, C. -X. Deng, B. Lu. (2003) Impairments in cerebellum Purkinje cells and motor function in mice lacking Smad4 in the central nervous system. J. Biol. Chem. 278, 42313 - 42320. 6. J. Du, L. Feng, E. Zaitsev, H. S. Je, X. Liu, and B. Lu. (2003) Activity- and tyrosine kinase-dependent facilitation of TrkB receptor internalization in hippocampal neurons. J Cell Biol. In press. 7. Z. G. Luo, H. -S. Je, F. Yang, W. C. Xiong, B. Lu, and L. Mei (2003) Activation of geranylgenanyltransferase is essential for Agrin-induced AChR clustering. Neuron In press. 8. S. X. Bamji, K. Shimazu, N. Kimes, J. Huelsken, W. Birchmeier, B. Lu, L. F. Reichardt. (2003) Regulation of presynaptic assembly and maintenance by -catenin. Neuron In Press. 9. F. Yang, X. He, J. Russell, and B. Lu. (2003) Ca2+ influx independent transmitter release mediated by mitochondrial Na+ -Ca2+ exchanger and protein kinase C. J. Cell Biol. In press. 2003 research articles
What Is the Purpose of Doing research? LANCE ARVSTRONG Publish or perish I Nature=10 JBC 2。00 Now York Times B It's Not about the bike My Journey fu照 I It is not about the number of papers I It is not about the impact factor of the papers I It is not about the nobel prize
What Is the Purpose of Doing Research? It is not about the number of papers It is not about the impact factor of the papers It is not about the Nobel Prize Publish or perish 1 Nature = 10 JBC
Basic elements for basic research SUMNER REDSINE PIESILEII IIL CEI IF IIEL CIIRESITILI PARAN SURVII TR STRAIGHI FROM THE GUT ACK WELCH WITH E WINY WITH 16 AGES OF PHOTOS Passion 6 Sigma Silver bullet test
Basic Elements for Basic Research Passion 6 Sigma Silver bullet test
Good Research Is the Key o My English is not good n They are biased against Chinese (foreigners) 人家没做过卡你就要做 口这是新的这是好的
Good Research Is the Key My English is not good They are biased against Chinese (foreigners) 人家没做过= 你就要做 这是新的 = 这是好的
What Is a first- Class paper/Research? o Major advancein a classic field 干细胞是如何分化成特定组织细胞的,胆固醇在人体的正常功用 n New techniques and methods that can be widely used 人类基因组研究中的自动测序技术,PCR, Patch clamp,Ca2+ Imaging, GFP n Discoveries with obvious practicalimplications AIDS virus receptor的发现,老年痴呆症基因的发现 o Conceptual brea kthrough, novelideas 神经营养因子可以促进学习记忆,RNA干扰现象 o Challenge to traditional views, break dogma 脑内有可分裂的神经干细胞,打破了传统观念 D Opening up new area, cross board “细胞凋亡”现象的发现,开辟了新的科研领域
What Is a First-Class Paper/Research? Major advance in a classic field 干细胞是如何分化成特定组织细胞的，胆固醇在人体的正常功用 New techniques and methods that can be widely used 人类基因组研究中的自动测序技术, PCR, Patch clamp, Ca2+ Imaging, GFP Discoveries with obvious practical implications AIDS virus receptor 的发现, 老年痴呆症基因的发现 Conceptual breakthrough, novel ideas 神经营养因子可以促进学习记忆, RNA干扰现象 Challenge to traditional views, break dogma 脑内有可分裂的神经干细胞，打破了传统观念 Opening up new area, cross board “细胞凋亡”现象的发现, 开辟了新的科研领域
What Is a Mediocre Paper/Research? Horizontal growth I made the discovery in rats, you find the same in cat. Filling gaps EGF activates JNK which is known to induce c-Jun expression. You show that EFG enhances c-Jun expression Working out details I found NO induces the production of cGMP, you work out dose response and time course Support existing idea,“ me too” EGF-R endocytosis requires dynamin, PDGF-Rtoo Follow up CREB binds to CrE. Working out CRE sequence. Incomplete study, preliminary
What Is a Mediocre Paper/Research? • Horizontal growth I made the discovery in rats, you find the same in cat. • Filling gaps EGF activates JNK which is known to induce c-Jun expression. You show that EFG enhances c-Jun expression. • Working out details I found NO induces the production of cGMP, you work out dose response and time course. • Support existing idea, “me too” EGF-R endocytosis requires dynamin, PDGF-R too. • Follow up CREB binds to CRE. Working out CRE sequence. • Incomplete study, preliminary
How to Read Scientific Papers? The Gilbert way Keep these in mind when you read What is the major question addressed in this paper? Is this question important and why? What are the approaches used in this paper, and whether they are adequate for the questions? What are the novel idea or using innovative approaches? What is the concept coming out of this paper? Do the results presented support this new concept? Weekly reading of CNS titles Critical, appreciative
How to Read Scientific Papers? • The Gilbert way • Keep these in mind when you read • What is the major question addressed in this paper? • Is this question important and why? • What are the approaches used in this paper, and whether they are adequate for the questions? • What are the novel idea or using innovative approaches? • What is the concept coming out of this paper? • Do the results presented support this new concept? • Weekly reading of CNS titles • Critical, appreciative