《离子通道生物学》课程教学课件（英文讲稿）Introduction

离子通道生物学细胞的跨膜信号转导Cheng Long, chenglong_scnu@qq.comSchool of Life Sciences, South China Normal UniversityFeb 28, 20121933华南師范大学INIVERSITYSOUTHCHINMANORMAL

离子通道生物学 细胞的跨膜信号转导 Cheng Long, chenglong_scnu@qq.com School of Life Sciences, South China Normal University Feb 28, 2012

Discusss about the definition ofthiscourse“lon channelology

“Ion channelology” Discuss about the definition of this course

Schedules2/28细胞膜电学性质、离子通道的分子结构与功能3/6Ca2+通道3/13Na+通道3/2oK+通道(SK,BKchannels)3/27C1-通道、配体门控的通道4/10细胞内Ca2+释放通道4/17离子通道疾病及其分子基础一(二)4/24离子通道疾病及其分子基础5/8膜片钳技术的理论5/15膜片钳技术的实践

2/28 细胞膜电学性质、离子通道的分子结构与功能 3/6 Ca2+通道 3/13 Na+通道 3/20 K+通道 (SK, BK channels) 3/27 Cl‐通道、配体门控的通道 4/10 细胞内Ca2+释放通道 4/17 离子通道疾病及其分子基础（一） 4/24 离子通道疾病及其分子基础（二） 5/8 膜片钳技术的理论 5/15 膜片钳技术的实践 Schedules

Score平时成绩课前预习，课堂发言、讨论，课后作业考试成绩：？闭卷？开卷

平时成绩： 课前预习，课堂发言、讨论，课后作业 考试成绩： ？闭卷 ？开卷 Score

ReferencesPatchclamping:anintroductory guideto patch clampelectrophysiology,ArelesMolleman,2003-175pages(ebook)Patch-clampapplicationsandprotocols,AlanA.Boulton,GlenB.Baker,WolfgangWalz-1995-316pagesElectrical propertiesof cells:patchclampforbiologists,LouisJDeFelice-1997-244pagesPatch-clampanalysis:advancedtechniques,WolfgangWalzAlanA.Boulton,GlenB.Baker-2002-345pageslonchannels:apracticalapproach,Richard H.Ashley-1995-302pageslon channels:molecules inaction,DavidJ.Aidley,PeterR.Stanfield-1996-307pagesIntroductiontoelectrophysiological methodsandinstrumentation,Franklin Bretschneider,Jan R.De Weille-2oo6-251pages

References  Patch clamping: an introductory guide to patch clamp electrophysiology, Areles Molleman, 2003 - 175 pages (ebook )  Patch-clamp applications and protocols , Alan A. Boulton, Glen B. Baker, Wolfgang Walz - 1995 - 316 pages  Electrical properties of cells: patch clamp for biologists , Louis J. DeFelice - 1997 - 244 pages  Patch-clamp analysis: advanced techniques , Wolfgang Walz, Alan A. Boulton, Glen B. Baker - 2002 - 345 pages  Ion channels: a practical approach , Richard H. Ashley - 1995 - 302 pages  Ion channels: molecules in action , David J. Aidley, Peter R. Stanfield - 1996 - 307 pages  Introduction to electrophysiological methods and instrumentation, Franklin Bretschneider, Jan R. De Weille - 2006 - 251 pages

ReferencesThe ion channel factsbook:Voltage-gated channels,Edward CConley, W. J. Brammar - 1999 - 860 pageslonchannelsandgeneticdiseases,DavidC.Dawson,SocietyofGeneralPhysiologists.Symposium,RaymondA.Frizzell-1995-178pagesLigand-and voltage-gatedion channels,R.AlanNorth-1995349pageslonchannelsofexcitablemembranes,BertilHille-2001-814pagesAxon_Guide.pdfEurBiophysJ2004,33,211-26ionchannels.pdfJMolCellCardiol2010,48,65-70CardiacCachannel.pdfPflugersArch-EyrJPhysiol2oo9,CardiacNachannel.pdfNatureRevNeurosci2007,8,451-65AP.pdfTrendsinNeuroscience2009,32,215-24TRPVchannel.pdf

References  The ion channel factsbook: Voltage-gated channels , Edward C. Conley, W. J. Brammar - 1999 - 860 pages  Ion channels and genetic diseases , David C. Dawson, Society of General Physiologists. Symposium, Raymond A. Frizzell - 1995 - 178 pages  Ligand- and voltage-gated ion channels , R. Alan North - 1995 - 349 pages  Ion channels of excitable membranes , Bertil Hille - 2001 - 814 pages  Axon_Guide.pdf  Eur Biophys J 2004, 33, 211-26 ion channels.pdf  J Mol Cell Cardiol 2010, 48, 65-70 Cardiac Ca channel.pdf  Pflugers Arch-Eyr J Physiol 2009, Cardiac Na channel.pdf  Nature Rev Neurosci 2007, 8, 451-65 AP.pdf  Trends in Neuroscience 2009, 32, 215-24 TRPV channel.pdf

ReferencesKartikVenkatachalam,CraigMontell(2007)TRPChannels.AnnualReviewofBiochemistry,76:387-417ProgressBiophysicsMoleBio2009,99,7-19NatRevDrugDiscov2009,8,153-71神经系统离子通道病，张黎明，科学出版社，2008年，465页离子通道学，李决、程芮，湖北科学技术，2007年，527页实用膜片钳技术，刘振伟编著，军事医科出版社，2006年心肌细胞离子通道和通道病，刘泰编著，人民卫生出版社，2006年离子通道药理学，杨宝峰主编，人民卫生出版社，2005年膜片钳实验技术，陈军，电子书心肌细胞电生理学：离子通道，离子载体和离子流，刘泰编著，人民卫生出版社，2005，252页细胞膜离子通道，刘安西，陈守同编译，中央民族学院出版社，1990年离子通道与疾病，张宗明，裘法祖.世界华人消化杂志，2005，13：585587

References  Kartik Venkatachalam, Craig Montell (2007) TRP Channels. Annual Review of Biochemistry, 76: 387-417  Progress Biophysics Mole Bio 2009, 99, 7-19  Nat Rev Drug Discov 2009, 8, 153-71  神经系统离子通道病, 张黎明, 科学出版社, 2008 年, 465 页  离子通道学, 李泱、程芮, 湖北科学技术, 2007 年, 527 页  实用膜片钳技术, 刘振伟 编著, 军事医科出版社, 2006 年  心肌细胞离子通道和通道病, 刘泰槰编著, 人民卫生出版社, 2006 年  离子通道药理学, 杨宝峰 主编, 人民卫生出版社, 2005 年  膜片钳实验技术, 陈军, 电子书  心肌细胞电生理学：离子通道，离子载体和离子流, 刘泰槰编著, 人民 卫生出版社, 2005, 252 页  细胞膜离子通道, 刘安西，陈守同编译, 中央民族学院出版社, 1990 年  离子通道与疾病, 张宗明, 裘法祖. 世界华人消化杂志, 2005, 13: 585- 587

Vital roles ofionchannelsInbasicphysiologicalfunctionsgeneration of electrical activity in nerves and musclecontrol of cardiac excitabilityintracellularsignalinghormonesecretioncell proliferationcellvolume regulationmanyotherbiologicalprocessesInanumberofpathophysiologicalconditionsepilepsyepisodicataxialongQTsyndromeBartter'ssyndromecysticfibrosis

Vital roles of ion channels  In basic physiological functions  generation of electrical activity in nerves and muscle  control of cardiac excitability  intracellular signaling  hormone secretion  cell proliferation  cell volume regulation  many other biological processes  In a number of pathophysiological conditions  epilepsy  episodic ataxia  long QT syndrome  Bartter’s syndrome  cystic fibrosis

Vital roles of ionchannelslon channels are drug targets fora number oftherapeutic agents developed forthe treatment ofvariousdiseasesNatchannel blockerssuchaslidocaineandlamotrigine are used as local anesthetic and to treatepilepsy,respectivelyCalciumchannel blockers suchas verapamil andnifedipine are usedforthetreatment of cardiacarrhythmiasandhypertension·ATP-dependentK+channelinhibitorssuchastolbutamide and glibenclamideareeffective inthetreatmentoftypell diabetes

Vital roles of ion channels  Ion channels are drug targets for a number of therapeutic agents developed for the treatment of various diseases.  Na+ channel blockers such as lidocaine and lamotrigine are used as local anesthetic and to treat epilepsy, respectively  Calcium channel blockers such as verapamil and nifedipine are used for the treatment of cardiac arrhythmias and hypertension  ATP-dependent K+ channel inhibitors such as tolbutamide and glibenclamide are effective in the treatment of type II diabetes

Thehistoryof ionchannelWenowknowthat,ionsarenotonlypossible,butacommon everyday experience,especiallyinaqueoussolutions such as those which surround living cells.Theconcept of an ion channelhadto await the acceptance bythe scientific community of the existence of ions as wellas the notion that cells are surrounded by a fattymembranebilayer.Thelate170s,Lavisouirandothers,physics,chemistryandbiologyTheemergenceofbothionandcell membraneconceptscanbetracedbacktothelate18o0s

The history of ion channel  We now know that, ions are not only possible, but a common everyday experience, especially in aqueous solutions such as those which surround living cells. The concept of an ion channel had to await the acceptance by the scientific community of the existence of ions as well as the notion that cells are surrounded by a fatty membrane bilayer.  The late 1700s, Lavisouir and others, physics, chemistry and biology  The emergence of both ion and cell membrane concepts can be traced back to the late 1800s