绪论色谱分离基础 Fundamental of chromatograph separation Section 1 overview 1. Separation techniques 2. History of chromatography 3. Classification of chromatography 4. The principle efchromatography. 5. Chromatogram and some terminology
绪论 色谱分离基础 1. Separation techniques 2. History of chromatography 3. Classification of chromatography 4. The principle of chromatography 5. Chromatogram and some terminology Fundamental of chromatograph separation Section 1 Overview
1. Separation techniques --→--1-1--→----1----1--- (a) Extraction: partition (L-L phase) (b)Distillation boiling point(L-G phase) (c) Participation solubility(L-s phase d) Chromatography consecutive use of separation steps 萃取〔分配)、蒸馏(沸点)、沉淀(溶解度)、 色谱(连续使用分离步骤)
1. Separation techniques (a) Extraction : partition (L-L phase) (b) Distillation : boiling point ( L-G phase) (c) Participation : solubility ( L-S phase) (d) Chromatography : consecutive use of separation steps 萃取（分配）、蒸馏（沸点）、沉淀（溶解度）、 色谱（连续使用分离步骤）
2. History of chromatography 溶剂 1906 Russian botanist Michail Tswett Calcium carbonate 剧碳酸钙 Extracts of leaf pigments色素 A色谐带 Chlorophylls叶绿素 Xanthophylls叶黄素 Spirilloxanthin螺旋黄质
2. History of chromatography 1906, Russian botanist Michail Tswett Calcium carbonate Extracts of leaf pigments 色素 Chlorophylls 叶绿素 Xanthophylls 叶黄素 Spirilloxanthin 螺旋黄质
3. Classification of chromatography 一-一 (1) internal chromatography planar: paper, thin-layer (11) external chromatography column: packing, capillary column 层析室 层析纸 组 份点 展开剂 ∈展开荆 层折板 纸层析装置 薄层层析装置
3. Classification of chromatography (ⅰ) internal chromatography : planar: paper, thin-layer (ⅱ) external chromatography : column: packing, capillary column
Classification of column chromatography according to the mobile and stationary phases used Stationary mobile phase phase Solid gaseous fluid liquid gas-solid supercriticalfluid liquid-solid GSC SFC LSC Liquid gas-liquid liquid-liquid GLC LLC
Classification of column chromatography according to the mobile and stationary phases used Stationary mobile phase phase Solid gaseous fluid liquid gas-solid supercriticalfluid liquid-solid GSC SFC LSC Liquid gas-liquid liquid-liquid GLC LLC
4. The principle of chromatography →--1------1--1--4--- Question 1: How chemically similar compounds can be separated in a chromatographic column? P2-3 sample mobile phase A+BEa B B ll B A目B呵
4. The principle of chromatography Question 1 : How chemically similar compounds can be separated in a chromatographic column? P 2-3 1 2 3 4 5 t t t t t sample mobile phase A+B B B B B A A A
5. Chromatogram and some terminology Base line Total retention time t-tM Dead time 20 Zone broadening 607h
5. Chromatogram and some terminology Base line Total retention time Dead time Zone broadening
用来衡量色谱峰宽度的参数,有三种表示方法: (1) Standard deviation (ii) The half-band width Y IM (111) The base-line width 0.607h 2
用来衡量色谱峰宽度的参数，有三种表示方法： (ⅰ) Standard deviation (ⅱ) The half-band width (ⅲ) The base-line width
Section 2 Chromatographic theory 1. Thermal dynamic equilibrium 2. Plate theory 3. Rate theory 4. The resolution
Section 2 Chromatographic theory 1. Thermal dynamic equilibrium 2. Plate theory 3. Rate theory 4. The resolution
-1--4--4-----4--1--4--1 色谱理论需要解决的问题: (1)色谱分离过程的热力学和动力学问题。 (2)影响分离及柱效的因素与提高柱效的途径。 (3)柱效与分离度的评价指标及其关系
色谱理论需要解决的问题： (1)色谱分离过程的热力学和动力学问题。 (2)影响分离及柱效的因素与提高柱效的途径。 (3)柱效与分离度的评价指标及其关系