1. INTRODUCTION 2. SAMPLE IDENTIFICATION & DATA MANAGEMENT FOR MICROBIOLOGY SAMPLES 3. RATE AND ACTIVITY MEASUREMENTS 4. COUNTING METHODS 5. BIOMASS AND BIOMARKER METHODS 6. MOLECULAR METHODS FOR INVESTIGATING DIVERSITY: CLONE LIBRARIES 6.1. DNA EXTRACTION FROM SEDIMENTS 6.2. PCR 6.3. SELECTING AND CONFIRMING EFFICACY OF 16S RRNA GENE PRIMERS 6.4. SELECTING AND USING PRIMERS FOR FUNCTIONAL GENES 6.5. CLONING, CLONE SELECTION AND SEQUENCING 6.6. SCREENING CLONE LIBRARIES FOR ARTEFACTS, CHIMERAS AND COVERAGE 6.7. PHYLOGENETIC ANALYSES 7. MOLECULAR METHODS FOR INVESTIGATING DIVERSITY: PROFILING METHODS 7.1. DENATURING GRADIENT GEL ELECTROPHORESIS (DGGE) FOR 16S RRNA GENES 7.2. BENTHIC DIVERSITY PROFILING OF BACTERIA USING ARISA 7.3. TERMINAL RESTRICTION FRAGMENT LENGTH POLYMORPHISM (T-RFLP) ANALYSIS 8. CULTURE METHODS 9. REFERENCES: FULL LIST

浙江大学医学院：《医学分子生物学 Molecular Cell Biology Gene》课程教学资源（PPT课件讲稿）导论（主讲：许正平）

◼ 前言 ◼ 基因的概念 ◼ 基因的化学本质 ◼ 人类基因和基因组的结构特点 ◼ 人类基因组计划

I. Overview of Regulation 8.1 Major modes of regulation II. Regulation of Enzyme Activity (Posttranslational level regulation) 8.2 Noncovalent enzyme inhibition 酶活的非共价抑制 8.3 Covalent Modification of Enzyme 酶的共价修饰 III. DNA-Binding Proteins and Regulation of Transcription by Negative and Positive Control (Transcriptional level regulation) 转录水平调控

III. DNA-Binding Proteins and Regulation of Transcription by Negative and Positive Control (Transcriptional level regulation) DNA结合蛋白与转录水平的调控 8.5 Negative control of transcription: Repression and Induction转录的负调控 8.6 Positive Control of Transcription ----Activator mediated regulation by protein-DNA binding IV. Global Regulation Mechanisms 8.7 Catabolite Repression ----Global control and the lac operon代谢分解物抑制 8.8 Other global control network 8.9 Quorum Sensing 群体感应

上海交通大学：《微生物学 Microbiology》课程教学资源（双语课件）Lecture 12.2 Microbial Genomics（Gene Function and Regulation）

上海交通大学：《微生物学 Microbiology》课程教学资源（双语课件）Lecture 11.2 Genetic Engineering（Gene Cloning）

上海交通大学：《生物技术与人类》通识课程教学资源（小论文）基因检测的原理、应?和伦理问题 The Principle,Application and Ethical Problem about Gene Detection

《基因与人 Genes and Human》课程参考书籍：《自私的基因 The Selfish Gene》（[英]里查德·道金斯）

第一节 基因诊断 第二节 基因治疗 基因治疗的概念 基因治疗的总体策略 基因治疗的基本程序 基因治疗的现状与展望