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1. The nucleic acids, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), are polymers of nucleotide units 1.1 DNA consists of four kinds of deoxyribonucleotide units linked together through covalent bonds 1.1.1 Each nucleotide unit is made of a nitrogenous base (the various part in the four different deoxyribonucleotides), a pentose sugar and a phosphate group
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随着cDNA微阵列和寡核苷酸芯片(下文没有特别说明时,统称为DNA微阵列)等 高通量检测技术的发展,我们可以从全基因组水平定量或定性检测基因转录产物mrNA 在本章中,基因表达数据特指基于DNA微阵列实验得到的反映mRNA丰度的数据,而 不包括基因表达最终产物—蛋白质丰度的数据。由于生物体中的细胞种类繁多,同时基因 表达具有时空特异性,因此,基因表达数据与基因组数据相比,要更为复杂,数据量更大, 数据的增长速度更快
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8.1 Some Basics 273 quence in the cells DNA. A segment of a DNA molecule 8.2 Nucleic Acid Structure 275 that contains the information required for the synthesis 8.3 Nucleic Acid Chemistry 291 8.4 Other Functions of Nucleotides 300 the only known func- Watson
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In vivo dna binding pattern of the polycomb Txn factor 1. What are the genes towhich it binds? 2. how does it affect theserenes? 3. What determines where itBinds??
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Gene mutation and evolution Gradual accumulation of mutations over long periods of time results in new biological species, each with a unique DNA sequence. Mutations and stabilization of mutations to new species. Charles Darwin: Unifying evolution theory-the origin of species from a common ancestor
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RNA polymerase transcribes genes How is this process regulated? How is specificity achieved? Key players transcriptional activator proteins -bind to specific sites on DNA and turn on the expression of nearby genes. Two adhesive surfaces one binds DNA, the other interacts with, and recruits RNA polymerase
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9.1基因是什么 9.2DNA的半保留复制 9.3RNA的组成和作用 9.4转录 9.5遗传密码的破译 9.6蛋白质的合成 9.7人类基因组计划
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How does it work? Ofloxacin belongs to a group of broad spectrum antibiotics called the quinolones. It works by entering the bacterial cell and inhibiting a chemical called DNA-gyrase which is involved in the production of genetic material (DNA). This therefore prevents the bacteria from reproducing
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11.1重组DNA技术是基因工程的核心技术 11.2获得需要的目的基因(外源基因) 11.3构建重组质粒和基因克隆 11.4转化受体细胞和转化子的筛选 11.5转化子的分析 Southern杂交
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一.是非题(共10分。只需注明“对”或“错”) 1.大多数嗜热菌的G-C含量高于中温菌。 2.大肠杆菌属低等原核生物,所以其遗传物质只是一条松散的环状双链DNA,不存在DNA高级结构
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