17.1 Introduction 17.2 The mating pathway is triggered by pheromone-receptor interactions 17.3 The mating response activates a G protein 17.4 Yeast can switch silent and active loci for mating type 17.5 The MAT locus codes for regulator proteins 17.6 Silent cassettes at HML and HMR are repressed 17.7 Unidirectional transposition is initiated by the recipient MAT locus 17.8 Regulation of HO expression 17.9 Trypanosomes switch the VSG frequently during infection 17.10 New VSG sequences are generated by gene switching 17.11 VSG genes have an unusual structure 17.12 The bacterial Ti plasmid causes crown gall disease in plants 17.13 T-DNA carries genes required for infection 17.14 Transfer of T-DNA resembles bacterial conjugation 17.15 Selection of amplified genomic sequences 17.16 Transfection introduces exogenous DNA into cells 17.17 Genes can be injected into animal eggs 17.18 ES cells can be incorporated into embryonic mice 17.19 Gene targeting allows genes to be replaced or knocked out

Leading References: Langer, P. Angew. Chem. Int. Ed. Engl. 2000, 39, 3049-3052. Ciganek, E. Org. React. 1997, 51, 201-350. Basavaiah, D.; et. al. Tetrahedron, 1996, 52, 8001-8062. Drewes, S. E.; Roos, G. H. P. Tetrahedron, 1988, 44, 4653-4670

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概念 实际中,fx)多样,复杂,通常只能观测到一些离散数据; 或者fx)过于复杂而难以运算。这时我们要用近似函数g(x来 逼近fx)

1.回流 在100ml圆底烧瓶中加入12ml 水，置烧瓶于冰水浴中，小心地将14ml浓硫 酸分多次加入烧瓶中，充分混合，在继续冷却下，加入9.1ml正丁醇，混合均匀， 然后将12.4g研细的溴化钠分多次加入烧瓶，每加一次必须充分旋动烧瓶以免结 块

Permission for the publication herein of Sadtler Standard Spectrahas been granted, and all rights are reserved. by Sadtler Research Laboratories, Division of Bio-Rad Laboratories, Inc. This book is printed on acid-free paper. g Copyright o 1998 by John Wiley Sons, Inc, All rights reserved Published simultancously in Canada

1996年，Autodesk公司在3d Studio 的基础上设计，是目前应用最为广泛的三 维设计软件之一。 如果主要用于建筑效果图制作，则 Pentium3的CPU、128M的内存、17英寸显 示器、1G的硬盘自由交换空间就可以

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Director: Guru Dutt Production: Guru Dutt Films Pvt. Ltd.; colour, 35mm; CinemaScope (first Indian CinemaScope production); running time: 150 minutes. Producer: Guru Dutt; screenplay and dialogue: Abrar Alvi; pho￾tography: V. K. Murthy; editor: Y. G. Chauhan; art director: M. R. Achrekar; sound: S. V. Rama; music: S. D. Burman; songs: Kaifi Azmi; costumes: Bhanumati. Cast: Baby Naaz (Pammy); Venna (Bina); Mahesh Kaul (Father-in￾law); Waheeda Rehman (Shanti); Guru Dutt (Suresh Sinha); Johnny