ge ne MetaCore data analysis suite and functional analysis oam0289a29658Reve
www.genego.com © 2008. GeneGo Inc. All Rights Reserved MetaCore data analysis suite and functional analysis
"Knowledge-based"functional data analysis ■圈美 Cancer relevant annotations,datatabases, Data parsing,normalization Active cpds analysis screening ·Knowledge Base: -protein interactions -causative associations (gene-disease,cpd-disease) pathways,protein complexes Experimental data depository -ontologies 1b Data analysis tools:EA,networks,interactome T●Biomarkers Y《Target ●●● Compound scoring
“Knowledge-based” functional data analysis HTS, HCS Cancer relevant annotations, datatabases, Active cpds analysis screening • Knowledge Base: - protein interactions - causative associations (gene-disease, cpd-disease) - pathways, protein complexes Experimental data depository - ontologies Data parsing, normalization Data analysis tools: EA, networks, interactome Biomarkers Target s Compound scoring
@O Knowledge base:Three interactions domains .700.000 compounds w/targets Ligands:metabolites,peptides,xenoboitics .4.000 endogenous metabolites .>20.000 ligand-receptor interactions .850 GPCRs and other membrane receptors Nuclear hormone receptors Membrane receptors Signal transduction: G proteins. >200K manually curated physical signaling interactions Secondary messengers 466 canonical maps 43,000 12-step canonical pathways Phosphotases Transcription factors 900 Hum ↓ 11,000 metabolic reactions Core effect:metabolic pathways 110 Fine metabolic maps ↓ Metabolites 4,000 endogenous metabolites
3 Knowledge base: Three interactions domains Signal transduction: G proteins, Secondary messengers Kinases Phosphotases Transcription factors Membrane receptors >200K manually curated physical signaling interactions 466 canonical maps 43,000 12-step canonical pathways 900 Human transcription factors 4100 target genes Core effect: metabolic pathways Metabolites 11,000 metabolic reactions 110 Fine metabolic maps 4,000 endogenous metabolites Ligands: metabolites, peptides, xenoboitics • 700,000 compounds w/targets • 4,000 endogenous metabolites •>20,000 ligand-receptor interactions •850 GPCRs and other membrane receptors • Nuclear hormone receptors
Pathways are assembled of interaction building blocks 30Y0+30意0+是0+流0息从。+S 00+2+0+是+多0+意水0≤
4 Pathways are assembled of interaction building blocks
Knowledge base:>100,000 biological pathways Ligand Ligand Ligand 0 Receptor Receptor 0 01 0 Adaptor 0 Second messenger Enzyme TF target Reaction ● TF Enzyme TE TF target metabolite
5 Pic.1 Ligand Receptor Adaptor Enzyme TF TF target Knowledge base: >100,000 biological pathways Ligand Receptor Second messenger Enzyme TF Ligand TF TF target Reaction metabolite
Distribution of interactions by mechanism 口mfa2nTg: binding:185725;52% -unspecified-;6693;2% transpos _口transport;797;0% transformation;929;% 口catalysis;14684;4% ■transcp280'5ao .▣Toxic effect:5445:2% ▣cleavage:2992:1% ■competitio:535:0% ▣Technical;2384s;7% Commple rec: -·mo袋nn 。co.of 9218:3% Phafec enssions
Distribution of interactions by mechanism
Molecular functions in Database Receptors ■Small GTPase 1827 326 2% 9% Transcriptional Factors ■Proteases 2999 1025 16% 5% _▣Transporters 2152 Phosphatase 11% ■iga Coacti ator of 9% 口Kinases_. 1284 7% G-protein regulators. Enrymes 1170 5261 27%
Molecular functions in Database
Compounds distribution by target ·Proteases12% Phospholipases 1% 口Transporters4% 口Phosphatases2% Binding Proteins 3% lon Channels 9% 口Kinases6% L口Receptors GPCR2B% ▣Enzymes24% ■Nuclear Receptors4%
Compounds distribution by target
O Protein complexes/groups(species-specific) Protein structure map(group,complex or group of complexes)is shown on protein page Protein details ame RL39_HUMAN/60S ribosomal protein L39 nonyms 50S ribosomal protein L39,RL39_HUMAN Genes Draw network Draw regulatory network from this protein Expand Collapse RL39_HUMAN 。Relation ecular function ·References Chosen protein highlighted PIR
Protein complexes/groups (species-specific) Protein structure map (group, complex or group of complexes) is shown on protein page Chosen protein highlighted
Ca Disease maps.Delta 508 degradation in cystic fibrosis ta09-CFTR ⊙ 80H42】 UFD1 nts pectra_F508 delta508-CFTR CFTR spectra_w tus B10 p value 8gaoRteRt tus_B10_FC ■ Retan HDAC6
Disease maps. Delta 508 degradation in cystic fibrosis