Helicobacter pylori(幽门螺杆菌) 角产 C Helicobacter pylori is a spiral-shaped gram negative rod .H. pylori consists of a large diversity of strains, and the genomes of three have been completely sequenced 8 H pylori is associated with antral gastritis 窦炎), duodenal( peptic) ulcer disease(十二指 肠溃疡), gastric ulcers(胃溃疡), and gastric carcinoma(胃癌)和MALT(胃粘膜相关B细胞 淋巴瘤) 5/3/2016 Helicobacter pylori-Junqi's Lecture for md students
5/3/2016 Helicobacter pylori-Junqi's Lecture for MD students 1 Helicobacter pylori (幽门螺杆菌) Helicobacter pylori is a spiral-shaped gramnegative rod H. pylori consists of a large diversity of strains, and the genomes of three have been completely sequenced H.pylori is associated with antral gastritis（胃 窦炎）, duodenal (peptic) ulcer disease（十二指 肠溃疡）, gastric ulcers（胃溃疡）, and gastric carcinoma（胃癌）和MALT（胃粘膜相关B细胞 淋巴瘤）
Helicobacter pylori, Hp 今1982 Warren和 Marshal)分离出“ unidentified curved bacilli ☆1984 Langenberg等命名为“ campylobacter- like organism,CLO” ☆1988 Thompson等发现其16 SrRNA序列分析相似于 Wolinella属” ☆1989G00dwin等发现5个分类学特征,命名“Hp” 幽门螺杆菌位于: 8-变形菌纲 (Epsilonproteobacteria) 螺杆菌科 helicobacterace)) 弯曲菌目( Campylobacterale 螺杆菌属( helicobacter) 5/3/2016 Helicobacter pylori-Junqi's Lecture for md students
5/3/2016 Helicobacter pylori-Junqi's Lecture for MD students 2 Helicobacter pylori，Hp 1982 Warren和Marshall分离出“unidentified curved bacilli” 1984 Langenberg等命名为“campylobacter- like organism, CLO” 1988 Thompson等发现其16S rRNA序列分析相似于Wolinella属” 1989 Goodwin等发现5个分类学特征，命名 “Hp” 幽门螺杆菌位于： ε-变形菌纲 （Epsilonproteobacteria） 弯曲菌目（Campylobacterales） 螺杆菌科Helicobacteraceae） 螺杆菌属（Helicobacter）
In 2005 the Karolinska Institute in Stockholm awarded the nobel prize in Physiology or Medicine to Marshall and Robin Warren, his long-time collaborator, for their discovery of the bacterium Helicobacter pylori and its role in gastritis and peptic ulce er disease 5/3/2016 Helicobacter pylori-Junqi's Lecture for md students
5/3/2016 Helicobacter pylori-Junqi's Lecture for MD students 3 In 2005, the Karolinska Institute in Stockholm awarded the Nobel Prize in Physiology or Medicine to Marshall and Robin Warren, his long-time collaborator, "for their discovery of the bacterium Helicobacter pylori and its role in gastritis and peptic ulcer disease
复 扫分丽 5/3/2016 Helicobacter pylori-Junqi's Lecture for md students
5/3/2016 Helicobacter pylori -Junqi's Lecture for MD students 4
生物学性状 今 x,、等醒 ◇革兰染色阴性螺旋弯曲形,细菌常排列成S形或海鸥状。 ◇一端带有2一6根鞭毛,运动活泼 ◆在胃黏膜上皮表面,胃小凹内 及腺腔内,呈不均匀的集团状 分布 5/3/2016 Helicobacter pylori-Junqi's Lecture for md students
5/3/2016 Helicobacter pylori-Junqi's Lecture for MD students 5 生物学性状 革兰染色阴性螺旋弯曲形，细菌常排列成S形或海鸥状。 一端带有2－6根鞭毛，运动活泼 在胃黏膜上皮表面，胃小凹内 及腺腔内，呈不均匀的集团状 分布
培养特征: 37°C,微需氧环境,营养要求高(血清或血细胞), 3~6天 生长特性: > oxidase- positive(氧化酶阳性) catalase- positive(触酶阳性) > Mobile(有动力) strong producer of urease(尿素酶)鉴定该菌的 主要依据之 5/3/2016 Helicobacter pylori-Junqi's Lecture for md students
5/3/2016 Helicobacter pylori-Junqi's Lecture for MD students 6 培养特征： 37 °C ，微需氧环境，营养要求高（血清或血细胞）， 3～6天 生长特性： oxidase-positive (氧化酶阳性) catalase-positive（触酶阳性） Mobile（有动力） strong producer of urease （尿素酶）鉴定该菌的 主要依据之一
致病机制 贲门切迹 底 食管腹段 贲门 十二指肠球部 幽门 胃小弯 角切迹 胃大弯 胃体 降段 幽门管 幽门窦 附图1-2胃的形态与分部 5/3/2016 Helicobacter pylori-Junqi's Lecture for md students
5/3/2016 Helicobacter pylori-Junqi's Lecture for MD students 7 致病机制
致病物质与致病机理 令定植( colonization)于胃黏膜上皮表面和胃黏液的底层。 ◆损害胃黏膜屏障 尿素酶( urase) 细胞毒素相关蛋白A( cytotoxin associated gene A,CagA PAⅠ编码 IV型分泌系统( Type IV secretion system) 空泡毒素( vacuolating toxin,vacA),细胞凋亡 5/3/2016 Helicobacter pylori-Junqi's Lecture for md students
5/3/2016 Helicobacter pylori-Junqi's Lecture for MD students 8 致病物质与致病机理 定植(colonization)于胃黏膜上皮表面和胃黏液的底层。 损害胃黏膜屏障 尿素酶(urase) 细胞毒素相关蛋白A(cytotoxin associated gene A, CagA) IV型分泌系统（Type IV secretion system） 空泡毒素(vacuolating toxin, vacA), 细胞凋亡 PAI 编码
Type IV secretion REVIEWS MICROBIOLOGY Anja Seubert, Rino Rappuoi and Antonello Cov CHIRON 5/3/2016 Helicobacter pylori-Junqi's Lecture for md students
5/3/2016 Helicobacter pylori -Junqi's Lecture for MD students 9
1.中性粒细胞浸润 2.粘膜上皮细胞损坏 ce: Ryan KJ, Ray CG: Sherris Medical Microbiology, Sth Edition: opyright e The McGraw-HIll Companies, Inc. All rights reserved. 5/3/2016 Helicobacter pylori-Junqi's Lecture for md students
5/3/2016 Helicobacter pylori-Junqi's Lecture for MD students 10 1. 中性粒细胞浸润 2. 粘膜上皮细胞损坏