Section II BacteriologyThe NeisseriaeSHIHEZI UNIVERSITY
Section III Bacteriology The Neisseriae SHIHEZI UNIVERSITY
KEY TERMS NeisseriaNeisseriae Diplococci Thayer Martin chocolate agar N. gonorrhoeae GonorrheaSexually transmitted disease, STDN. meningitidlisMeningitisSHIHEZI UNIVERSITY
KEY TERMS Neisseria Neisseriae Diplococci Thayer Martin chocolate agar N. gonorrhoeae Gonorrhea Sexually transmitted disease, STD N. meningitidis Meningitis SHIHEZI UNIVERSITY
NeisseriaGram negative, diplococcusN. meningitidisN. gonorrhoeaeN. siccaN. subflavaN. flavescensN. mucosaSHIHEZI UNIVERSITY
Neisseria Gram negative, diplococcus N. meningitidis N. gonorrhoeae N. sicca N. subflava N. flavescens N. mucosa SHIHEZI UNIVERSITY
Neisseria gonorrheaGonococcusSHIHEZI UNIVERSITY
Neisseria gonorrhea Gonococcus SHIHEZI UNIVERSITY
Morphology0.8 μ m, Gram negative, diplococci (pairs of cocci most in neutrophil cellsSHIHEZIUNIVERSITY
Morphology 0.8μm, Gram negative, diplococci (pairs of cocci) most in neutrophil cells SHIHEZI UNIVERSITY
Figure21-1.Gramstainofaurethralexudateofapatientwithgonorhea.Nucleiandcellmembraneoutinesofmanypolymorphonuclearcellsareseen(twshownbylargearows).lntracellulargram-negativediplococci (Neisseria gonorrhoeae) in clumps aremarked by thesmall arrows.SHIHEZIUNIVERSITY
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Neisseragonorrhoeae-coccoidprokarye divdingcausesgonha(SEMx40000)DrDennisKunkel,UniversityofHawaiUsed with permissionSHIHEZIUNIVERSITY
Neisseria gonorrhoeae - coccoid prokaryote (dividing); causes gonorrhea (SEM x 40,000) © Dr Dennis Kunkel, University of Hawaii. Used with permission SHIHEZI UNIVERSITY
CultivationCultureObligatory aerobicThese bacteria grow best on chocolate agar so-calledbecause it contains heated blood brown in color Thayer Martin Chocolate Agar A modified (selective) chocolate agar Biological reactions+Glucose fermentation onlyCan not use other sugarsSHIHEZI UNIVERSITY
Cultivation Culture Obligatory aerobic These bacteria grow best on chocolate agar so-called because it contains heated blood brown in color Thayer Martin Chocolate Agar A modified (selective) chocolate agar Biological reactions Glucose fermentation only Can not use other sugars SHIHEZI UNIVERSITY
Oxidase Test The colonies areoxidase positivewhich isdemonstrated byflooding the platewith a dye which onoxidation changescolorPhoto by Karen M.KiscrSHIHEZI UNIVERSITY
Oxidase Test The colonies are oxidase positive which is demonstrated by flooding the plate with a dye which on oxidation changes color SHIHEZI UNIVERSITY
Surface Antigenic StructuresOutermembranePeptidoglycanCell envelopeCytoplasmicPeptidoglycanmembraneOutermembraneCytoplasmicmembranePilusPilusFigure21-2.CollageanddrawingofNgonorrhoeaeshowing piliand the threelayers ofthe cellenvelopeSHIHEZIUNIVERSITY
Surface Antigenic Structures SHIHEZI UNIVERSITY