Section III BacteriologyEnteric Gram Negative Rods -EnterobacteriaceaesSHIHEZI UNIVERSITY
Section III Bacteriology Enteric Gram Negative Rods - Enterobacteriaceaes SHIHEZI UNIVERSITY
KEY TERMSEnterobacteriaceaeShigellaOpportunistic diseases DiarheaBacilry dysenteryShiga toxinI Dysentery Urinary tract infections Salmonella enteritidisLactose positive/negativeSalmonellosisEnteropathogenicE.coliSalmonella cholerae-suisEnterotoxigenic E.coliSalmonlla typhiTyphoid Heat stable toxinVi Heat labile toxinEnteroinvasive E. coliYersinia entercolitica Enterohemorrhagic E.coliVibrio choleraeVero toxin (Shiga-like)Choleragen (cholera toxinHemolysinCampylobacter junipiliHelicobacter pyloriSHIHEZIUNIVERSITY
KEY TERMS Enterobacteriaceae Opportunistic diseases Shigella Diarrhea Bacillary dysentery Dysentery Shiga toxin Urinary tract infections Salmonella enteritidis Lactose positive/negative Salmonellosis Enteropathogenic E. coli Salmonella cholerae-suis Enterotoxigenic E. coli Salmonella typhi Heat stable toxin Typhoid Heat labile toxin Vi Enteroinvasive E. coli Yersinia entercolitica Enterohemorrhagic E. coli Vibrio cholerae Vero toxin (Shiga-like) Choleragen (cholera toxin) Hemolysin Campylobacter jejuni pili Helicobacter pylori SHIHEZI UNIVERSITY
Bacteria InfectedGastrointestinal TractEnterobacteriaceaeescherichia. shigella. salmonella enterobacterklebsiella, serratia, proteus, and others Vibrio CampylobacterHelicobacterSHIHEZIUNIVERSITY
Bacteria Infected Gastrointestinal Tract Enterobacteriaceae escherichia, shigella, salmonella, enterobacter, klebsiella, serratia, proteus, and others Vibrio Campylobacter Helicobacter SHIHEZI UNIVERSITY
(family)The Enterobacteriaceae A large, heterogeneous group of gram-negative rods whosenatural habitat is the intestinal tract of humans and animals The family includes many generaRegularly PathogensOpportunistic PathogensEscherichiaShigllaSalmonellaEnterobacterKlebsillaSerratiaProteusSHIHEZI UNIVERSITY
The Enterobacteriaceae (family) A large, heterogeneous group of gram-negative rods whose natural habitat is the intestinal tract of humans and animals The family includes many genera Regularly Pathogens Shigella Salmonella Opportunistic Pathogens Escherichia Enterobacter Klebsiella Serratia Proteus SHIHEZI UNIVERSITY
The EnterobacteriaceaeFacultative anaerobes or aerobesFerment a wide range of carbohydratesPossess a complex antigenic structureProduce avariety of toxins and othervirulence factorsEnterobacteriaceaeColiformsEnteric gram-negative rodsEnteric bacteriaSHIHEZI UNIVERSITY
The Enterobacteriaceae Facultative anaerobes or aerobes Ferment a wide range of carbohydrates Possess a complex antigenic structure Produce a variety of toxins and other virulence factors Enterobacteriaceae Enteric gram-negative rods Enteric bacteria Coliforms = SHIHEZI UNIVERSITY
Taxonomy of Enterobacteriaceae Complex and rapidly changingSince the introduction of techniques thatmeasure evolutionary distance, such as nucleicacid hybridization and sequencing More than 25 genera and 110 species orgroups have been definedClinically significant Enterobacteriaceaecomprise 20-25 speciesOther species are encountered infrequentlySHIHEZI UNIVERSITY
Taxonomy of Enterobacteriaceae Complex and rapidly changing Since the introduction of techniques that measure evolutionary distance, such as nucleic acid hybridization and sequencing More than 25 genera and 110 species or groups have been defined Clinically significant Enterobacteriaceae comprise 20–25 species Other species are encountered infrequently SHIHEZI UNIVERSITY
MorphologyShort gram-negative rodsTypical morphology is seen in growth onsolid media in vitrobut morphology is highly variable in clinicalspecimensCapsulesKlebsiella: large and regularOther species: uncommonSHIHEZI UNIVERSITY
Morphology Short gram-negative rods Typical morphology is seen in growth on solid media in vitro but morphology is highly variable in clinical specimens Capsules Klebsiella: large and regular Other species: uncommon SHIHEZI UNIVERSITY
E.coliSHIHEZI UNIVERSITY
SHIHEZI UNIVERSITY
CultureE coli and most of the other enteric bacteriaform circular, convex, smooth colonieswith distinct edgesThe salmonellae and shigellae producecolonies similar to E coli but do notferment lactoseSHIHEZI UNIVERSITY
Culture E coli and most of the other enteric bacteria form circular, convex, smooth colonies with distinct edges The salmonellae and shigellae produce colonies similar to E coli but do not ferment lactose SHIHEZI UNIVERSITY
Active Biochemical ReactionsTbmcmoeergmaCrobacterfteandiEnterolacterderoganesEscherichiacolKieb sela meunoniaeKlebiela aytocaMorganellamaganlPateusmiratilsSamanellaChokrasuksSalmanelaTyphiSalnonlamostserotypsSematiamarcexensShigello someiS dysenterioeSfexneni,SboydTAdapted fromFarne196421:46,SHIHEZIUNIVERSITY
Active Biochemical Reactions SHIHEZI UNIVERSITY