Section III BacteriologyVibrios, Campylobacter, HelicobacterSHIHEZI UNIVERSITY
Section III Bacteriology Vibrios, Campylobacter, Helicobacter SHIHEZI UNIVERSITY
KEY TERMS Vibrio choleraeCholeragen Vero toxin (Shiga-like(cholera toxin)Campylobacter HemolysinJejunipiliHelicobacter pyloriYersinia entercoliticaSHIHEZI UNIVERSITY
KEY TERMS Vibrio cholerae Vero toxin (Shiga-like) Choleragen (cholera toxin) Hemolysin Campylobacter jejuni pili Helicobacter pylori Yersinia entercolitica SHIHEZI UNIVERSITY
Bacteria InfectedGastrointestinal TractEnterobacteriaceaeescherichia. shigella. salmonella enterobacterklebsiella, serratia, proteus, and others Vibrio CampylobacterHelicobacterSHIHEZIUNIVERSITY
Bacteria Infected Gastrointestinal Tract Enterobacteriaceae escherichia, shigella, salmonella, enterobacter, klebsiella, serratia, proteus, and others Vibrio Campylobacter Helicobacter SHIHEZI UNIVERSITY
VibiosVibio choleraKINGCHOLERAVibio parahaemolyticusSHIHEZI UNIVERSITY
Vibios Vibio cholera Vibio parahaemolyticus SHIHEZI UNIVERSITY
The Vibrios Vibrios are among the most common bacteria insurface waters worldwide They are curved aerobic rods and are motilepossessing a polar flagellumV cholerae serogroups O1 and 0139 causecholera in humans+while other vibrios may cause sepsis or enteritisSHIHEZIUNIVERSITY
The Vibrios Vibrios are among the most common bacteria in surface waters worldwide They are curved aerobic rods and are motile, possessing a polar flagellum V cholerae serogroups O1 and O139 cause cholera in humans while other vibrios may cause sepsis or enteritis SHIHEZI UNIVERSITY
VibriosGram negative rods Comma shapedFacultative anaerobes Oxidase positive Simple nutritional requirements Readily cultivateSHIHEZI UNIVERSITY
Vibrios Gram negative rods Comma shaped Facultative anaerobes Oxidase positive Simple nutritional requirements Readily cultivate SHIHEZI UNIVERSITY
CultureThiosulfate-citrate-bile-sucrose (TCBSagar1 oxidase-positivegrow at a very high pH (8.5-9.5)+ rapidly killed by acid.SHIHEZI UNIVERSITY
Culture Thiosulfate-citrate-bile-sucrose (TCBS) agar oxidase-positive grow at a very high pH (8.5–9.5) rapidly killed by acid. SHIHEZI UNIVERSITY
Antigenic Structure & BiologicClassification V cholerae has OTable 18-1. The medically important vibrioslipopolysaccharidesthat confer serologicOrganismHuman DiseasespecificityVcholerae serogroups 01Epidemic and pandemicThere are at least 139and 0139choleraO antigen groupsVcholerae serogroupsCholera-ike diarhea mildV cholerae strains ofnon-01/non-0139diarrhea; rarely, extraintestinalO group 1 and Oinfectiongroup 139 causeclassic choleraV parahaemolyticusGastrentrtis perhapsextraintestinalinfection occasionally, non-01/non- 0i39 V Otherscholerae causesV mimicus,Vvulnifcus,Eawoundofttissueancholera-like diseaseVhliae fuialis,other extraintestinalV damsela, V anginolyinfections,all uncommonticus,VmetschnikoviSHIHEZIUNIVERSITY
Antigenic Structure & Biologic Classification V cholerae has O lipopolysaccharides that confer serologic specificity There are at least 139 O antigen groups V cholerae strains of O group 1 and O group 139 cause classic cholera occasionally, non- O1/non- O139 V cholerae causes cholera-like disease SHIHEZI UNIVERSITY
Serotypes & BiotypesV cholerae serogroup O1 antigen has determinantsthat make further typing:The main serotypes are Ogawa and InabaTwo biotypes of epidemic V cholerae have been definedclassic and EI TorThe EITor biotype producesahemolysin gives positive resultson the Voges-Proskauer test, and is resistant to polymyxinBMolecular techniques can also be used to type V choleraeTyping is used for epidemiologic studies, and testsgenerally are done only in reference laboratoriesSHIHEZI UNIVERSITY
Serotypes & Biotypes V cholerae serogroup O1 antigen has determinants that make further typing: The main serotypes are Ogawa and Inaba Two biotypes of epidemic V cholerae have been defined, classic and El Tor The El Tor biotype produces a hemolysin, gives positive results on the Voges-Proskauer test, and is resistant to polymyxin B. Molecular techniques can also be used to type V cholerae Typing is used for epidemiologic studies, and tests generally are done only in reference laboratories SHIHEZI UNIVERSITY
Occurrence - choleraCholera,2002-2003Third world?Common+ Food hygiene* Ingestion ofsea-food Other locations* Travelers,uncommonCountrieslareasreporingcholeracaseCountrieswithimportedcholeracasesSHIHEZI UNIVERSITY
Occurrence - cholera Third world Common * Food hygiene * Ingestion of sea-food * Other locations * Travelers, uncommon SHIHEZI UNIVERSITY