Changes in the Eye Microbiota associated with Contact Lens Wearing Hakdong Shin, Kenneth Price, Luong Albert Jack Dodick,Lisa Park, Maria Gloria Dominguez-Bello 陈藤蔡婷陈坷璇刘穹
background result scussIon
Genus Pseudomonas 假单胞菌属 Haemophilus 嗜血杆菌属 Streptococcus 链球菌属 Methylobacterium 甲基杆菌属 Lactobaci∥ls 乳酸菌属 Acinetobacter 不动杆菌属 Staphylococcus 葡萄球菌属 Corynebacterium 棒状杄菌属
O cular Defensive role microbiota mostly coagulasenegative Colonization of pathogens Over 50% growth staphylococci, Propionibacterium, and in the skin-like bacteria Culture-dependent additional Pseudomonas, Bradyrhizobium Aquabacterium, Sphingomonas, Streptococcus, Streptophyta, quencing workCcby-sa3.0,https:// fromen.wikipediatoCommonsbyKauczukusingCommonshelper,CcbY-sa3.,https:/commons.wikimediaorg/w/indexphp?curid=4916101 p//pic35nipic.com/20131109/1246704817115327410 tp: //easyread ph 126. net/qropBurbEtThz05oMQQQrw==/7917002689475514117-jpg
By The original uploader was Lady Weaxzezz at English Wikipedia - Transferred from en.wikipedia to Commons by Kauczuk using CommonsHelper., CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=4916101 By Aney - Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=290822 http://pic35.nipic.com/20131109/12467048_171153274104_2.jpg http://easyread.ph.126.net/qropBurbEt7hz05oMQQQrw==/7917002689475514117.jpg Culture-dependent Sequencing growth of skin-like bacteria mostly coagulasenegative staphylococci, Propionibacterium, and Corynebacterium Pseudomonas, Bradyrhizobium, Acinetobacter, Brevundimonas, Aquabacterium, Sphingomonas, Streptococcus, Streptophyta, Methylobacterium, Enhydrobacter, Bacillus, and Ralstoniaspp additional Over 50% Defensive role against Colonization of pathogens in the eye
A risk factor for inflammatory eye conditions Microbial involvement and interacti 100 million host conjunctival epithelium worldwide Bacterial contamination of contact lenses 16s rrNA *V4 region contact gene sequencing lenses surveys Microbiota y 58 subjecs ,o non-lens wearers 28 lens wearers ocular surface 7, 010,096 sequences Structure function with an average of 21, 569 reads per sample 11, 750 OTUs yielded By-70InxownworkCcBy3.0,https://commons.wikimediaorg/w/indexphp?curid=7929385
~ 100 million worldwide A risk factor for inflammatory eye conditions Microbial involvement and interaction with host conjunctival epithelium Bacterial contamination of contact lenses By - טל איתןOwn work, CC BY 3.0, https://commons.wikimedia.org/w/index.php?curid=7929385 58 subjects 7,010,096 sequences 11,750 OTUs yielded 30 non-lens wearers 28 lens wearers with an average of 21,569 reads per sample without singletons ocular surface *V4 region
PD( phylogenetic diversity )whole tree otu es No orU ut on 20 Number of species Faith D P Conservation evaluation and phyloge rsity]. Biological Conservation, 1992, 61(1): 1
Faith D P. Conservation evaluation and phylogenetic diversity[J]. Biological Conservation, 1992, 61(1):1-10. http://www.berrygenomics.com/wp-content/uploads/2014/12/otu_tree.png
Effect of Anesthetic eye drops PD Whole Tree Observed species 53g5m25四9m Sequences Per Sample uences Per Sampl a diversity Sampling location Laboratory(n=116, conjunctiva Ophthalmology practice(n=75, conjunctiva Fig S1A
Fig.S1A α diversity
Effect of Anesthetic eye drops LDA Phylum-level Genus-level P:g_Pseu Fusobacteria* Cyanobacteria" f Enterobacteriaceae: g Bacteroidetes* P: g_ Neisseria Actinobacteria P: f Oxalobacteraceae Other* P: g_Sphingobium F:g_Anaerococcus :g Streptococcus Fig_Lactobacillus* F:g_Staphylo C; o_Streptophyta; f_:g A; g Rothia Laboratory Ophthalmologic Ophthalmologic g_coryneb Unassigned practice Laboratory practice Conjunctiva Conjunctiva Conjunctiva Conjunctiva n=116 n=75 n=116 Fig S1B
Fig.S1B LDA
Effect of Anesthetic eye drops Unweighted Weighted Pc2(4%) O Ophthalmologic practice Conjunctiva (N=75) O Ophthalmologic practice Conjunctiva(N=75) O Laboratory Conjunctiva(N=116) gl Ophthalmolog O Laboratory Conjunctiva( N=116) Laboratory a Aphtha malacic +-- aboratory 02040.60.8 p=0.001 Pc1(13% Pc1(54%) Fig S1C
Fig.S1C
Figure. PD Whole Tree Observed species Non-lens wearers Conjunctiva Lens wearers Non-lens wearers (n=63) Skin under the eve Lens wearers (n=51) Lens wea Contact lens 300 400 Phylogenetic diversity Number of observed species T-test
T-test
