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河南师范大学:《生物化学》课程PPT教学课件(英文版)Section G RNA synthesis and processing

G1 RNA structure G2 Transcription in prikaryotes G3 The lac operon G4 The trp operon G5 Transcription in eukaryotes G6 Transcription of protein-coding genes G7 Regulation of transcription by RNA pol II G8 Processing of eukaryotic pre-mRNA G9 Ribosomal RNA G10 Transfer RNA
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G RNA synthesis and processing G1 RNA structure G2 Transcription in prokaryotes G3 The lac operon G4 The tro operon G5 Transcription in eukaryotes G6 Transcription of protein-coding genes G7 Requlation of transcription by RNA pol l G8 Processing of eukaryotic pre-mRNA G 9 Ribosomal RNA G10 Transfer rna

• G1 RNA structure • G2 Transcription in prikaryotes • G3 The lac operon • G4 The trp operon • G5 Transcription in eukaryotes • G6 Transcription of protein-coding genes • G7 Regulation of transcription by RNA pol II • G8 Processing of eukaryotic pre-mRNA • G9 Ribosomal RNA • G10 Transfer RNA G RNA synthesis and processing

Central Dogma Replication DNA Reverse Transcription Transcription RNA Translation Protein Juang RH(2004)BCbasics

Central Dogma RNA DNA Protein Transcription Translation Replication Reverse Transcription Juang RH (2004) BCbasics

G1 RNA Structure Pentose Nucleoside Nucleic acid-Nucleotide Bases A Phosphoric acid Purine G Bases C Pyrimidine

Nucleotide Nucleoside Phosphoric acid Pentose Bases Bases Purine Pyrimidine A G C U G1 RNA structure Nucleic acid

Infrequent bases s4U4-硫尿嘧啶 Inosine 次黄嘌呤 m1G1-甲基鸟嘌呤 m1A1-甲基腺嘌呤 DHU5,6-二氢尿嘧啶 s2C2-硫胞嘧啶 Pseudouridine(ψ)假尿嘧啶:C1-c5

Infrequent bases • s4U 4-硫尿嘧啶 • Inosine 次黄嘌呤 • m1G 1-甲基鸟嘌呤 • m1A 1-甲基腺嘌呤 • DHU 5,6-二氢尿嘧啶 • s2C 2-硫胞嘧啶 • Pseudouridine(ψ )假尿嘧啶:C1-C5

Rare bases HC HN HN N ON N HoN N N Ribose Ribose Ribose 4-Thiouridine(SU) Inosine (I) 1-Methylguanosine (m'G) CHs NH--CHo-CH-C CHs N HIN HIN Ribose HN H N O N Ribose H Ribose ribe Dose N -Isopentenyladenosine(i'A) Ribothymidine(T) Pseudouridine ( f) Dihydrouridine()

Rare bases

Nucleoside(C1-N1 or C1-N9 Adenosine Guanosine Cytidine Uridine Pseudouridine(u): C1-C5

Nucleoside(C1-N1 or C1-N9) • Adenosine • Guanosine • Cytidine • Uridine • Pseudouridine(ψ):C1-C5

Covalent structure ° long polymer chain ATPUTPGTPCTP 3-5 phosphodiester bonds(not 2-5) 5 -GUCATUATCCA-3

Covalent structure • long polymer chain • ATP,UTP,GTP,CTP • 3’-5’ phosphodiester bonds(not 2’-5’) • 5’-GUCATUATCCA-3’

Long polymer chain ase base OH OH sr 0、0 35 00 0-0

Long polymer chain

O-P=0 O-P=0 CH2O、Base1 HH H CH2O、Base1 HH 微碱 -H:: OH 断裂 CH2O、「B ase CHoO Base2 RNase HH HH O OH O OH O-P=O CH2O、「Ba ase CH2O、「Base3 HH O OH: O OH O-P=0 a/3 RNA O-P=0

断裂 RNase 微碱

Difference between rna and dna 一一s

Difference between RNA and DNA

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