Medical Microbiology Syllabus(2013) Key laboratory of Medical Molecular Virology of Ministries of Education and Health department of medical Microbiology and Parasitology Shanghai Medical College, Fudan University The aim of Medical Microbiology course is to introduce basic principles and application relevance of clinical disease for students who are in preparation for physicians. The content of rigorous course includes many etiological agents responsible for global infectious diseases. It covers all biology of bacteria, viruses and other pathogens related with infectious diseases in humans. In order to foster a students ability to analyze and solve problems in their future clinical career, the course will provide the conceptual basis for understanding pathogenic microorganisms and particularly address the fundamental mechanisms of their It will als informatic and diagnostic skills in microbiology, including the practical application and interpretation of laboratory tests for the diagnosis of infectious diseases STUDENT: Clinical Medicine(6 years)MBBS TEXKBOOK: Jawetz, Melnick, Adelberg s Medical Microbiology, 26th(Medical Microbiology Professors: Qu Di, Yuan Zhenhong Jiang Shibo, Xie Youhua. Chen Li, Cai Qiliang Associate professors &Ph. D. Long Jianer, Niu Chen, Cao Wenjun, Zhao Chao, Pan Tingting, Liu Jing, Ding Yuena, Han Wendong, Sun Zhipin, Cai Xia, Song Wuhui, Zhang Junqi, Wang Yuyan Teaching assistants: Zhang Junqi: jungizhang@fudan. edu.cr WangYuyan:yuvanss@fudan.edu.cn COURSE OBJECTIVES Knowledge 1. Define principles of microbial taxonomy, structure, physiology, genetics immunology and pathogenes 2. Develop a knowledge of microbial organisms and their relevance of infectious 3. Understand the principles of prevention and treatment of pathogenic microorganism infection in humans skills Familiar skill of the laboratory use in diagnose infections, including appropriate specimen collection and selection of tests 2. Develop the ability to manipulate the laboratory tests to identify pathogeni microorganisms, 3. Understand the principles of the laboratory tests in diagnosis and identification of
1 / 11 Medical Microbiology Syllabus (2013) Key laboratory of Medical Molecular Virology of Ministries of Education and Health & Department of Medical Microbiology and Parasitology Shanghai Medical College, Fudan University The aim of Medical Microbiology course is to introduce basic principles and application relevance of clinical disease for students who are in preparation for physicians. The content of rigorous course includes many etiological agents responsible for global infectious diseases. It covers all biology of bacteria, viruses and other pathogens related with infectious diseases in humans. In order to foster a student’s ability to analyze and solve problems in their future clinical career, the course will provide the conceptual basis for understanding pathogenic microorganisms and particularly address the fundamental mechanisms of their pathogenicity. It will also provide opportunities for a student to develop both informatic and diagnostic skills in microbiology, including the practical application and interpretation of laboratory tests for the diagnosis of infectious diseases. STUDENT: Clinical Medicine (6 years) MBBS TEXKBOOK ：Jawetz, Melnick, & Adelberg‘s Medical Microbiology, 26 th (Medical Microbiology) Professors： Qu Di, Yuan Zhenhong, Jiang Shibo, Xie Youhua., Chen Li, Cai Qiliang Associate professors & Ph.D.: Long Jianer, Niu Chen, Cao Wenjun, Zhao Chao, Pan Tingting, Liu Jing, Ding Yuena, Han Wendong, Sun Zhipin, Cai Xia, Song Wuhui, Zhang Junqi, & Wang Yuyan Teaching assistants: Zhang Junqi: junqizhang@fudan.edu.cn; Wang Yuyan: yuyanss@fudan.edu.cn COURSEOBJECTIVES Knowledge 1．Define principles of microbial taxonomy, structure, physiology, genetics， immunology and pathogenesis; 2．Develop a knowledge of microbial organisms and their relevance of infectious diseases; 3．Understand the principles of prevention and treatment of pathogenic microorganism infection in humans. Skills 1．Familiar skill of the laboratory use in diagnose infections, including appropriate specimen collection and selection of tests; 2．Develop the ability to manipulate the laboratory tests to identify pathogenic microorganisms; 3．Understand the principles of the laboratory tests in diagnosis and identification of
pathogenic microorganisms PROFESSIONALISM OBJECTIVES Our Departments of Microbiology endeavor to educate cand idates who will pursuit the Doctor of Med icine degree with the following professional objectives for their successful career. A strong desire to practice these objectives in Medical Microbiology(and in other aspects of undergraduate med ical education ) will substantially enhance a student's qualification in acquisition of knowledge, humility, and technical skills for a successful and reward ing career 1. Demonstrate respect for others a. Students are asked to attend all class meetings, particularly small group exercises b Students respect in a dignified manner to faculty, staff, and student colleagues exemplifying their maturity and empathy c. Students agree to abide by biosafety practices when in BSL-2 exercises 2. Exhibit honesty and integrity a Students abide by the honor Code of Fudan University in all exams, quizzes, and graded assignments b. Students discuss honestly in any grading oversights or computer/technology errors with Course faculty as soon as any discrepancies arise 3. Practice concern through communication, respect, and understanding a. Students grasp opportunities to"rehearse professional etiquette when dealing with faculty in situations of uncertainty and/or ignorance b Students appreciate the enormous benefits of faculty variation in education and/or d which facilitates understand ing of diverse population c. Students encourage their classmates to heightened levels of understanding by promoting questions and answer through sharing personal experiences 4. Perform assignments responsibly and with account ability a. Students complete the assignments of each lecture, according to the Section chedule b. Students arrive promptly at classes as well as laboratory activities c. Students work effectively as team members when assigned, realizing they gair more when fully engaged in teamwork d Students seize the opportunity to learn from a diverse group of faculty instructors in small group settings, focused on basic relevance of clinical cases 5. Seek excellence and scholarship a. Students strive to excel at all four areas of Med ical Microbiology, including immunology, bacteriology, mycology, and virology b. Students read to supplement the foundational scaffold of information presented in the lectures c. Students seek to develop treatment behaviors that will reduce the incidence of antimicrobial resistance in the world
2 / 11 pathogenic microorganisms PROFESSIONALISM OBJECTIVES: Our Departments of Microbiology endeavor to educate candidates who will pursuit the Doctor of Medicine degree with the following professional objectives for their successful career. A strong desire to practice these objectives in Medical Microbiology (and in other aspects of undergraduate medical education) will substantially enhance a student’s qualification in acquisition of knowledge, humility, and technical skills for a successful and rewarding career. 1. Demonstrate respect for others a. Students are asked to attend all class meetings, particularly small group exercises. b. Students respect in a dignified manner to faculty, staff, and student colleagues, exemplifying their maturity and empathy. c. Students agree to abide by biosafety practices when in BSL-2 exercises. 2. Exhibit honesty and integrity a. Students abide by the Honor Code of Fudan University in all exams, quizzes, and graded assignments. b. Students discuss honestly in any grading oversights or computer/technology errors with Course faculty as soon as any discrepancies arise. 3. Practice concern through communication, respect, and understanding a. Students grasp opportunities to “rehearse” professional etiquette when dealing with faculty in situations of uncertainty and/or ignorance. b. Students appreciate the enormous benefits of faculty variation in education and/or background which facilitates understanding of diverse populations. c. Students encourage their classmates to heightened levels of understanding by promoting questions and answer through sharing personal experiences. 4. Perform assignments responsibly and with account ability a. Students complete the assignments of each lecture, according to the Section schedule. b. Students arrive promptly at classes as well as laboratory activities. c. Students work effectively as team members when assigned, realizing they gain more when fully engaged in teamwork. d. Students seize the opportunity to learn from a diverse group of faculty instructors in small group settings, focused on basic relevance of clinical cases. 5. Seek excellence and scholarship a. Students strive to excel at all four areas of Medical Microbiology, including immunology, bacteriology, mycology, and virology. b. Students read to supplement the foundational scaffold of information presented in the lectures. c. Students seek to develop treatment behaviors that will reduce the incidence of antimicrobial resistance in the world
COURSE ORGANIZATION bacteriology Spirochete, Rickettsiae, virology Mycology Chlamydia Mycoplasma 12 basic lectures 1 basic lecture 13 basic lectures basic lecture 10 lab experiments 1 lab demonstration iments 1 lab demonstration Interactive discussions Interactive discussions The teaching methods below will be used to ensure student mastery of the knowledge 1. Lectures will cover the main learning objectives for each section, and are supplemented with (1)Quizzes, or review questions (2)Discussion on clinical cases and correlations with news of emerging diseases (3)Clinical diagnostic exercises 2. Small Groups: During each section of the course, small group discussions focus tailored to the student's heightened understanding of pertinent topics. These discussion groups encourage integration and review of basic concepts, but new information will be delivered and deeper discernment will be accomplished in these class meetings. Topics presented in small group discussions will be covered in examinations; therefore attendance is required at all meetings(sign-in sheets shall be monitored) 3. Quizzes or review questions: Each lecture provides quizzes, or review questions to help students digest and correlate of all course materials. This facil itates the student,'s ability to formalize concept integration throughout the course and will be overed in examinations. Therefore students are required to do the homework and hand it in to teachers; it will contribute to the final grade 4. Examinations: Midterm section will culminate with a non-cumulative. written multiple-choice test and questions where students demonstrate attainment of learning objectives. (Learning objectives comprise the first section of each lecture's ASSESSMENT Requirements are the same as those of the chinese students in Shanghai Medical School (pass-excellent, good, pass/ or fail- makeup examination) Student performance in Medical Microbiology will be evaluated by a series of exams and quizzes. Attendance at all course events is necessary for optimal performance, and material presented in small group sessions will be graded and shall be included in quizzes and exams In the light of pod-casting, students are reminded that confusion in lectures is most efficiently cleared up immed iately by questions at the end of the lecture rather than at later times
3 / 11 COURSE ORGANIZATION Bacteriology Spirochete, Rickettsiae, Chlamydia & Mycoplasma Virology Mycology 12 basic lectures 10 lab experiments Interactive discussions 1 basic lecture 1 lab demonstration 13 basic lectures 3 lab experiments Interactive discussions 1 basic lecture 1 lab demonstration The teaching methods below will be used to ensure student mastery of the knowledge: 1. Lectures will cover the main learning objectives for each section, and are supplemented with: (1) Quizzes, or review questions (2) Discussion on clinical cases and correlations with news of emerging diseases (3) Clinical diagnostic exercises 2. Small Groups: During each section of the course, small group discussions focus on issues tailored to the student's heightened understanding of pertinent topics. These discussion groups encourage integration and review of basic concepts, but new information will be delivered and deeper discernment will be accomplished in these class meetings. Topics presented in small group discussions will be covered in examinations; therefore attendance is required at all meetings (sign-in sheets shall be monitored). 3. Quizzes or review questions: Each lecture provides quizzes, or review questions to help students digest and correlate of all course materials. This facilitates the student's ability to formalize concept integration throughout the course and will be covered in examinations. Therefore students are required to do the homework and hand it in to teachers; it will contribute to the final grade. 4. Examinations: Midterm section will culminate with a non-cumulative, written, multiple-choice test and questions where students demonstrate attainment of learning objectives. (Learning objectives comprise the first section of each lecture’s handout.) ASSESSMENT Requirements are the same as those of the Chinese students in Shanghai Medical School (pass-excellent, good, pass/ or fail- makeup examination) Student performance in Medical Microbiology will be evaluated by a series of exams and quizzes. Attendance at all course events is necessary for optimal performance, and material presented in small group sessions will be graded and shall be included in quizzes and exams. In the light of pod-casting, students are reminded that confusion in lectures is most efficiently cleared up immediately by questions at the end of the lecture rather than at later times
Grades are not determined for ind ividual exams. Final grades are determined ccord ing to the ratios in the following Lecture-60%(midterm exam20%+final test 40%) E Attendance -10%(everyone should be signature on the namelist in each lecture) Homework-10%(each lecture gives 2-3 questions, students choose those as homework, submit the homework to elearning before the deadline(in 2 weeks after the lecture) This lecture introduces common terms and basic concepts of med ical microbiology, in order to gain an awareness of the versatility of microbes and understand of how we coexist BACTERIOLOGY Lecture Two Bacterial Morphology and structures, Metabolism growth 1. Basics of bacterial cell wall structures and properties associated with bacterial cell walls( Gram positive/negative cell wall, staining, Peptidoglycan, LPS pathogenesis, and targets of antibiotics) 2. Structures of the bacterial cell and the genetic makeup of bacteria(ribosome, Plasmid, Chromosome, target of antibiotics, roles in pathogenesis or drug 3. Morphology of bacteria and the versatility of bacteria(in clinical diagnosis) 4. Bacterial appendages, bacterial spores, capsules, etc, and their related with clinic 5. Techniques to study morphology of bacteria 6. Bacterial growth, survival and death, cultivation of bacteria, growth curve bacterial metabolism. classification of bacteria Lecture Three bacterial Heredity &variation 1. Genetic material of bacteria( Chromosome, plasmid, transposable element integron and phage genome) 2. Bacterial phages, virulent phage and lysogenic phage 3. Mechanisms of transfer and recombination of bacterial genes(transformation, ransduction, conjugation and lysogenic conversion; gene mutation 4. The significance of bacterial genetic variation(in drug resistance, pathogenesis or virulence and variation, diagnosis, and vaccination), and manipulation of cloned DNA 5. Sterilization and disinfection(concepts and methods) 6. Biosafety in the medical microbiology laboratory Lecture Four Bacterial Infection Pathogenesis 1. Normal human microbiota(Role of the resident microbiota, and locations in the body) 2. Virulence of bacteria, bacterial virulence factors and their regulation(exotoxin endotoxin, and their contribution to pathogenesis
4 / 11 Grades are not determined for individual exams. Final grades are determined according to the ratios in the following： Lecture-60%（midterm exam20% + final test 40%）; Experiment-20%; Attendance -10% (everyone should be signature on the namelist in each lecture); Homework-10%（each lecture gives 2-3 questions, students choose 10 of those as homework, submit the homework to elearning before the deadline (in 2 weeks after the lecture). This lecture introduces common terms and basic concepts of medical microbiology, in order to gain an awareness of the versatility of microbes and understand of how we coexist. BACTERIOLOGY Lecture Two Bacterial Morphology and Structures，Metabolism & Growth 1. Basics of bacterial cell wall structures and properties associated with bacterial cell walls (Gram positive/negative cell wall, staining, Peptidoglycan, LPS, pathogenesis, and targets of antibiotics) 2. Structures of the bacterial cell and the genetic makeup of bacteria (Ribosome, Plasmid, Chromosome, target of antibiotics, roles in pathogenesis or drug resistance) 3. Morphology of bacteria and the versatility of bacteria (in clinical diagnosis) 4. Bacterial appendages, bacterial spores, capsules, etc., and their related with clinic practice. 5. Techniques to study morphology of bacteria 6. Bacterial growth, survival and death, cultivation of bacteria, growth curve, bacterial metabolism, classification of bacteria. Lecture Three Bacterial Heredity &Variation 1. Genetic material of bacteria (Chromosome, plasmid, transposable element, integron and phage genome). 2. Bacterial phages, virulent phage and lysogenic phage 3. Mechanisms of transfer and recombination of bacterial genes (transformation, transduction, conjugation and lysogenic conversion); gene mutation. 4. The significance of bacterial genetic variation (in drug resistance, pathogenesis or virulence and variation, diagnosis, and vaccination), and manipulation of cloned DNA. 5. Sterilization and disinfection (concepts and methods) 6. Biosafety in the medical microbiology laboratory Lecture Four Bacterial Infection & Pathogenesis 1．Normal human microbiota (Role of the resident microbiota, and locations in the human body) 2．Virulence of bacteria, bacterial virulence factors and their regulation (exotoxin, endotoxin, and their contribution to pathogenesis)
3. Infection process(development, and outcomes) 4. Nosocomial infections and their prevention Lecture Five Immune Responses to bacterial Infections 1. Immunity and immune responses against bacterial infection 2. Mechanisms of innate immunity(barriers, phagocytes, complement system etc 3. Mechanisms of specific host defense(humoral immunity, cell-mediated immunity heir activities on exocellular or intracellular bacterial infection Lecture Six Antimicrobial Chemotherapy, drug resistance and its prevention, Laboratory Diagnosis, Control of bacterial diseases 1. Antimicrobial Chemotherapies and their targets 2. Drug resistance, drug-bacteria relationship, clinical implications, and prevention 3. Diagnostic medical microbiology(collect samples, culture, iden diagnosis, immunologic or molecular diagnostic tests 4. Prevention of bacterial infection( active immunization, vaccines, and passive immunization) Lecture Seven Cocci 1. Staphylococci(pyogenic cocci and coagulase-negative staphylococcus) () Morphology, culture, and biological characteristics of Staphylococcus aureus (2)The virulence factors of Staphylococcus aureus and their effects includ ing SPa coagulase, hemolysin, and enterotoxin) ()The diagnostic laboratory tests for Staphy lococcus aureus and the principles of controlling Staphylococcus infections 2. Streptococcus(classification (1)Morphology, culture, and biological characteristics of Streptococcus (2) The virulence factors of group A streptococcus, the pyogenic and non- pyogenic infections caused by group A streptococcus, diseases caused by group B, D streptococcus and enterococcus (streptoly n, pyrogenic exotoxin, Invasive (3) The diagnostic laboratory tests for streptococcus and pneumococcus antistreptolysin O test(ASO test) (1)Classification of Neisseria(Neisseria meningitides and Neisseria gonorrhoeae) (2)The biolog ical characteristics and pathogenicity of-and immune response to Neisseria meningitides reatment of the d iseases caused by Neisseria meningitides (4) Neisseria gonorrhoeae and infection Lecture Eight Enterobacteriaceae: General introduction, Escherichia coli Shigella 1. General characterization and classification of Enterobacteriaceae 2. Escherichia coli
5 / 11 3．Infection process (development, and outcomes) 4．Nosocomial infections and their prevention Lecture Five Immune Responses to bacterial Infections 1. Immunity and immune responses against bacterial infection 2. Mechanisms of innate immunity (barriers, phagocytes, complement system etc.) 3. Mechanisms of specific host defense (humoral immunity, cell-mediated immunity, their activities on exocellular or intracellular bacterial infection. Lecture Six Antimicrobial Chemotherapy, drug resistance and its prevention, Laboratory Diagnosis, Control of bacterial diseases 1. Antimicrobial Chemotherapies and their targets 2. Drug resistance, drug-bacteria relationship, clinical implications, and prevention 3. Diagnostic medical microbiology (collect samples, culture, identification, rapid diagnosis, immunologic or molecular diagnostic tests ) 4. Prevention of bacterial infection ( active immunization, vaccines, and passive immunization) Lecture Seven Cocci 1. Staphylococci (pyogenic cocci and coagulase-negative staphylococcus) (1) Morphology, culture, and biological characteristics of Staphylococcus aureus (2) The virulence factors of Staphylococcus aureus and their effects ( including SPA, coagulase, hemolysin, and enterotoxin) (3) The diagnostic laboratory tests for Staphylococcus aureus and the principles of controlling Staphylococcus infections 2. Streptococcus (classification) (1) Morphology, culture, and biological characteristics of Streptococcus (2) The virulence factors of group A streptococcus, the pyogenic and non- pyogenic infections caused by group A streptococcus, diseases caused by group B, D streptococcus and enterococcus (streptolysin, pyrogenic exotoxin, invasive enzymes) (3) The diagnostic laboratory tests for streptococcus and pneumococcus; antistreptolysin O test (ASO test) 3. Neisseria (1) Classification of Neisseria (Neisseria meningitides and Neisseria gonorrhoeae) (2) The biological characteristics and pathogenicity of - and immune response to - Neisseria meningitides (3) Principles of diagnostic laboratory tests, and principles of prevention and treatment of the diseases caused by Neisseria meningitides (4) Neisseria gonorrhoeae and infection Lecture Eight Enterobacteriaceae: General introduction, Escherichia coli & Shigella 1. General characterization and classification of Enterobacteriaceae 2. Escherichia coli
(1)Diseases induced by Escherichia coli, including pathogenesis and Clinical Findings (2) Diagnostic laboratory tests for Escherichia coli-associated diseas (3)Treatment, Epidemiology, Prevention Control of enterobacteriaceae associated diseases 3. Shigella (1)Serological classification of Shigella, pathogenesis and pathology (2)Diagnostic laboratory tests and immunity of Shigella-associated diseases (3) Treatment, Epidemiology, Prevention and Control of the diseases induced by Shigella Lecture Nine Enterobacteriacea: Samonella, Vibrios, Campylobacter, Helicobacter 1. Salmonella (1) The Morphology, Identification and Classification of salmonella (2)Pathogenesis and clinical find ing for the disease induced by salmonella (3)Diagnostic laboratory tests for the diseases caused by Salmonella, including bacteriologic methods for isolation of salmonella, serologic methods (4)Treatment, Epidemiology, Prevention and Control, including carriers, source of infection(water, milk and other dairy products, shellfish, dried or frozen eggs, meats and meat products, animal dye and household pets) 2. Klebsiella and proteus and infections 3. vibrios (1) The main biological characteristics of Vibrio cholerae 1. Pathogenesis of the diseases induced by Vibrio cho lerae and the immunity (vibrio cholerae enterotoxin and function in the disease) 2) Diagnostic laboratory tests for the d iseases induced by vibrio cholerae () Treatment, Epidemiology, Prevention and Control of the diseases induced by the Vibrio cholerae (4)Overview of Vibrio parahaemolyticus Other Vibrios, Aeromonas and Plesiomonas 4. Campylobacters (1) The main biological characteristics of Campylobacter (2) Diseases caused by Campylobacter, includ ing pathogenesis and pathology (3) Diagnostic laboratory tests for the d iseases caused by Campylobacter (4) Epidemiology and control of diseases caused by Campylobacter 5. Helicobacter (1) The main biological characteristics of the Helicobacter species (2) Pathogenesis and pathology of the Helicobacter species-associated diseases (3) Diagnostic laboratory tests for the d iseases induced by Helicobacter species Lecture Ten Mycobacteria &C diphtheria 1. Mycobacterium tuberculosis (1)The main biological characteristics of M. tuberculosis (2)Pathogenesis of M tuberculosis, Koch's phenomenon
6 / 11 (1) Diseases induced by Escherichia coli, including pathogenesis and Clinical Findings (2) Diagnostic laboratory tests for Escherichia coli -associated diseases (3) Treatment, Epidemiology, Prevention and Control of Enterobacteriaceaeassociated diseases 3. Shigella (1) Serological classification of Shigella, pathogenesis and pathology (2) Diagnostic laboratory tests and immunity of Shigella-associated diseases (3) Treatment, Epidemiology, Prevention and Control of the diseases induced by Shigella Lecture Nine Enterobacteriacea: Samonella, Vibrios, Campylobacter, Helicobacter 1. Salmonella (1) The Morphology , Identification and Classification of Salmonella (2) Pathogenesis and clinical finding for the disease induced by Salmonella (3) Diagnostic laboratory tests for the diseases caused by Salmonella, including bacteriologic methods for isolation of salmonella, serologic methods (4) Treatment, Epidemiology, Prevention and Control, including carriers, source of infection (water, milk and other dairy products, shellfish, dried or frozen eggs, meats and meat products, animal dye and household pets) 2. Klebsiella and Proteus and infections 3. Vibrios (1) The main biological characteristics of Vibrio cholerae 1. Pathogenesis of the diseases induced by Vibrio cholerae and the immunity (Vibrio cholerae enterotoxin and function in the disease) (2) Diagnostic laboratory tests for the diseases induced by Vibrio cholerae (3) Treatment, Epidemiology, Prevention and Control of the diseases induced by the Vibrio cholerae (4) Overview of Vibrio parahaemolyticus & Other Vibrios, Aeromonas and Plesiomonas 4. Campylobacters (1) The main biological characteristics of Campylobacter (2) Diseases caused by Campylobacter, including pathogenesis and pathology (3) Diagnostic laboratory tests for the diseases caused by Campylobacter (4) Epidemiology and control of diseases caused by Campylobacter 5. Helicobacter (1) The main biological characteristics of the Helicobacter species (2) Pathogenesis and pathology of the Helicobacter species-associated diseases (3) Diagnostic laboratory tests for the diseases induced by Helicobacter species Lecture Ten Mycobacteria & C. diphtheria 1. Mycobacterium tuberculosis (1) The main biological characteristics of M. tuberculosis (2) Pathogenesis of M. tuberculosis, Koch’s phenomenon
()Diagnosis of M. tuberculosis infection, treatment and prevention 2. Mycobacterium leprae leprosy 3. C. diphtheria and infections( discussion) Lecture Eleven. Anaerobic bacteria 1 Overview: Clostridia, non-spore forming anaerobes (1) The main biolog ical characteristics of Clostridia (C. tetani, C. botulinum and C perfringens) (2) Infection and Pathogenesis of Clostridia(tetanospasmin, and botulinum toxin toxin of C. perfringens )and immunity (3)Diagnostic laboratory tests for the diseases cause by clostridia (4) Treatment and prevention of Clostridia diseases 2. Infection caused by non-spore forming anaerobes Lecture twelve Some other important bacteria Overview: biological characters, infection and immunity, pathogenesis and diagnosis treatment and prevention 1. infection caused by Hemophilusinfluenza 2. Yersinia pestis and Plagu 3. Bordetella and brucella 4. Bacillus anthracis. Bacillus cereus 5. Legionellae pneumophila Lecture Thirteen Spirochetes, Rickettsiae, Chlamydia, Actinomyce tes, Mycoplasma and cell wall-defective bacteria Overview: biolog ical characteristics, infection and immunity, pathogenesis and diagnosis, treatment and prevention of the microbial infection 1. Spirochetes (1)Leptospira Leptospirosis (2) Treponema pallidum syphilis ()Borrelia burgdorferi lyme disease 2. Rickettsiae the diseases 3. Chlamydia (1)Chlamydia trachoma, genital infection and inclusion conjunctivitis (2) Chlamydia pneumoniae respiratory infections (3)Chlamydia psittaci psittacosi 4. Actinomycetes, nocardiosis and actinomycetoma 5. Mycoplasma and cell wall-defective bacteria (1) Mycoplasma pneumoniae atypical pneumonias (2) Ureaplasma urealyticum (3)Cell wall-defective bacteria Ⅴ IROLOGY Lecture fourteen Viral Classification, Structure Multiplication neral pro 2. Principles of virus structure and function(genome, capsid, envelope) 7/11
7 / 11 (3) Diagnosis of M. tuberculosis infection, treatment and prevention 2. Mycobacterium leprae & leprosy 3. C. diphtheria and infections (discussion) Lecture Eleven. Anaerobic Bacteria 1. Overview: Clostridia , non-spore forming anaerobes (1) The main biological characteristics of Clostridia (C. tetani, C. botulinum and C. perfringens). (2) Infection and Pathogenesis of Clostridia (tetanospasimin, and Botulinum toxin, toxin of C. perfringens ) and immunity (3) Diagnostic laboratory tests for the diseases cause by Clostridia (4) Treatment and prevention of Clostridia diseases 2. Infection caused by non-spore forming anaerobes Lecture Twelve Some other important bacteria Overview: biological characters, infection and immunity, pathogenesis and diagnosis, treatment and prevention. 1. infection caused by Hemophilusinfluenza, 2. Yersinia pestis and Plague 3. Bordetella and Brucella, 4. Bacillus anthracis, Bacillus cereus 5. Legionellae pneumophila Lecture Thirteen Spirochetes, Rickettsiae, Chlamydia, Actinomycetes, Mycoplasma and cell wall-defective bacteria Overview: biological characteristics, infection and immunity, pathogenesis and diagnosis, treatment and prevention of the microbial infection 1. Spirochetes (1) Leptospira & Leptospiriosis (2) Treponema pallidum & Syphilis (3) Borrelia burgdorferi & Lyme disease 2. Rickettsiae & the diseases 3. Chlamydia (1) Chlamydia trachoma, genital infection and inclusion conjunctivitis (2) Chlamydia pneumoniae & respiratory infections (3) Chlamydia psittaci & psittacosis 4. Actinomycetes, norcardiosis and actinomycetoma 5. Mycoplasma and cell wall-defective bacteria (1) Mycoplasma pneumoniae & atypical pneumonias (2) Ureaplasma urealyticum (3) Cell wall-defective bacteria VIROLOGY Lecture Fourteen Viral Classification, Structure & Multiplication 1. General properties of viruses 2. Principles of virus structure and function (genome, capsid, envelope)
3. Reaction to physical and chemical agents 4. Viral Multiplication and stages of replication(adhesion, entry, uncoating, synthesis, assembly and release) Lecture Fifteen Viral Heredity &variation 1. Genetics of animal viruses 2. Mechanisms of viral gene mutation 3. Defective interfering particle, defective virus 4. Classification of viruses Lecture Sixteen Mechanisms of viral pathogenesis 1. Viral infection and transmission 2. Mechanism of viral pathogenesis: cytopathic effect, immune pathogenesis and Immune escape 3. Forms of viral infection, viral persistent infection(viral horizontal transmission and vertical transr characteristics of chronic virus infection. latent virus infection, and slow virus infection) 4. Viral infection and tumors Lecture Seventeen: Human Immune Response to viral Infections Control of viral diseases 1. Innate immunity: IFN classification, characteristics, and anti-viral activity: function of nK cells and complement against viral infection 2. Specific cellular immunity: two antigen presenting pathways to activate t cells, anti-viral mechanisms of CTL 3. Antibody production and its mechanisms of action against viral infection; concepts of Neutralizing antibody, Haemagglutination inhib ition antibodies Complement fixation antibody 4. Principles of viral vaccine design and development 5. Anti-viral chemotherapy and mechanisms 6. Clinical application of anti-viral drugs nucleoside analogs, amantad ine, Interferon, protease inhibitor, Chinese herbal medicine and gene therapy Lecture Eighteen Laboratory diagnosis of virus Infection Antivirus Therapy 1. Laboratory Diagnosis of Virus Infection (1)Specimen collection and submission (2) Cultivation assays for virus (3)Purification identification of virus (4)Serological tests and rapid diagnostic tests 2. Prevention and treatment of viral infection (1)Antivirus Chemotherapy (IFN, nucleoside analogs, reverse transcriptase inhibitors protease inhibitors, fusion inhibitors, etc (2)Viral vaccines(Killed -virus vaccine, live-virus vaccine) Lecture Nineteen Orthomyxovirus and Paramyxovirus
8 / 11 3. Reaction to physical and chemical agents 4. Viral Multiplication and stages of replication(adhesion, entry, uncoating, synthesis, assembly and release) Lecture Fifteen Viral Heredity &Variation 1. Genetics of animal viruses 2. Mechanisms of viral gene mutation 3. Defective interfering particle, defective virus 4. Classification of viruses Lecture Sixteen Mechanisms of Viral Pathogenesis 1. Viral infection and transmission 2. Mechanism of viral pathogenesis：cytopathic effect, immune pathogenesis and immune escape 3. Forms of viral infection, viral persistent infection (viral horizontal transmission and vertical transmission; the characteristics of chronic virus infection, latent virus infection, and slow virus infection) 4. Viral infection and tumors Lecture Seventeen: Human Immune Response to viral Infections & Control of viral diseases 1. Innate immunity：IFN classification, characteristics, and anti-viral activity； function of NK cells and complement against viral infection 2. Specific cellular immunity：two antigen presenting pathways to activate T cells, anti-viral mechanisms of CTL 3. Antibody production and its mechanisms of action against viral infection； concepts of Neutralizing antibody, Haemagglutination inhibition antibodies, Complement fixation antibody 4. Principles of viral vaccine design and development 5. Anti-viral chemotherapy and mechanisms 6. Clinical application of anti-viral drugs ： nucleoside analogs, amantadine, Interferon, protease inhibitor, Chinese herbal medicine and gene therapy Lecture Eighteen Laboratory Diagnosis of Virus Infection & Antivirus Therapy 1． Laboratory Diagnosis of Virus Infection (1) Specimen collection and submission (2) Cultivation & assays for virus (3) Purification & identification of virus (4) Serological tests and rapid diagnostic tests 2. Prevention and treatment of viral infection (1) Antivirus Chemotherapy (IFN, nucleoside analogs, reverse transcriptase inhibitors, protease inhibitors, fusion inhibitors, etc.) (2) Viral vaccines (Killed –virus vaccine, live-virus vaccine) Lecture Nineteen Orthomyxovirus and Paramyxovirus
1. Orthomyxovirus (1) The main biological characteristics of Orthomyxovirus (2)Influenza virus infections in human (3)Evolution of influenza virus and flu pandemics (4) Diagnostic laboratory tests for Orthomyxovirus 2. Paramyxovir () The main biological characteristics of Paramyxovirus (2) Parainfluenza virus infection (3)Respiratory Syncytial virus infections (4) Measles virus infections (5)Mumps virus infections (6)Hendra virus and Nipah virus infections ()Other viruses causing respiratory infections: Rubella, adenovirus, rhinovirus coronavirus. etc Lecture Twenty Gastrointestinal Virus and rhinovirus Contents of course 1. General properties and overview of Picornaviruses (enteroviruses) (1)Properties of polioviruses (2)Pathogenicity and immunity of polioviruses ( Laboratory diagnosis, prevention control of polioviruses (4)Coxsackieviruses (5)Echoviruses and new enteroviruses 2. Acute gastroenteritis-associated viruses (1)General properties of rotaviruses (2)Pathogenicity and immunity of rotaviruses (3)laboratory diagnosis of rotavirus infections (4)Caliciviruses infections(Noroviruse) (6) Enteric adenoviruses and reovirus infection Lecture twenty-one Hepatitis virus B and Hepatitis virus D I. Hepatitis B virus (1)The main biological characteristics of Hepatitis B virus(genome, replication) (2) Profiles of Hepatitis B virus infection (chronic infection) (3)Diagnostic laboratory tests for Hepatitis B infection (4) Treatment, prevention control of hepatitis B infection 2. Hepatitis d virus (1)Properties of Hepatitis d virus (2) Hepatitis D virus infection and control Lecture Twenty-two Hepatitis viruses A, C and e 1. Hepatitis virus a (1) Properties of Hepatitis A virus(genome (2) Hepatitis A virus infections ()Prevention& control of hepatitis A infection
9 / 11 1. Orthomyxovirus (1) The main biological characteristics of Orthomyxovirus (2) Influenza virus infections in human (3) Evolution of influenza virus and flu pandemics (4) Diagnostic laboratory tests for Orthomyxovirus 2. Paramyxovirus (1) The main biological characteristics of Paramyxovirus (2) Parainfluenza virus infection (3) Respiratory Syncytial virus infections (4) Measles virus infections (5) Mumps virus infections (6) Hendra virus and Nipah virus infections (7) Other viruses causing respiratory infections： Rubella, adenovirus、rhinovirus, coronavirus, etc. Lecture Twenty Gastrointestinal Virus and Rhinovirus Contents of Course 1. General properties and overview of Picornaviruses （enteroviruses） (1) Properties of polioviruses (2) Pathogenicity and immunity of polioviruses (3) Laboratory diagnosis, prevention & control of polioviruses (4) Coxsackieviruses (5) Echoviruses and new enteroviruses 2. Acute gastroenteritis-associated Viruses (1) General properties of rotaviruses (2) Pathogenicity and immunity of rotaviruses (3) laboratory diagnosis of rotavirus infections (4) Caliciviruses infections (Noroviruse) (5) Astroviruses infections (6) Enteric adenoviruses and reovirus infection Lecture Twenty-one Hepatitis virus B and Hepatitis virus D 1. Hepatitis B virus (1) The main biological characteristics of Hepatitis B virus (genome, replication) (2) Profiles of Hepatitis B virus infection （chronic infection） (3) Diagnostic laboratory tests for Hepatitis B infection (4) Treatment, prevention & control of hepatitis B infection 2. Hepatitis D virus (1) Properties of Hepatitis D virus (2) Hepatitis D virus infection and control Lecture Twenty-two Hepatitis viruses A，C and E 1. Hepatitis virus A (1) Properties of Hepatitis A virus (genome) (2) Hepatitis A virus infections (3) Prevention & control of hepatitis A infection
2. Hepatitis virus C (1)The main properties of Hepatitis C virus(genome) (2)Hepatitis C virus infection (chronic infection ()Diagnosis, treatment, prevention control of hepatitis C infections 3. Hepatitis virus E (1) The main properties of Hepatitis e virus (2)Hepatitis E virus infection (3)Prevention control of hepatitis E infections Lecture Twenty-three Athropod-borne and rodent-borne viral diease 1. Togavirus and Flavivirus Encephalitis (1)The main properties of Togavirus and Flavivirus (2)Transmission cycle of Japanese B encephaliti (3)Pathogensis and clinical profiles of Togavirus and Flavivirus infections (4)Laboratory Diagnosis of Japanese B encephalitis (5) Treatment, prevention control of Japanese B encephalitis (6)Dengue virus and dengue hemorrhagic fever ()Other viral encephalitis( West nile fever, etc.) 2. Viral hemorrhagic fever (1)Main properties of Viral hemorrhagic fever (2)Hantavirus and hemorrhagic fever with renal syndrome and pulmonary syndrome (3)Lassa fever etc Lecture Twenty-four Retrovirus Human Immunodeficiency virus 1. Concept of Retro 2. Major biological properties of HI V: including morphology and structure, genome and replication cycle 3. HIV Pathogenic mechanism and Immunological function 4. HIV laboratory diagnostic tests and the principles of prevention and treatment 5. Human T-cell lymphotropic virus(HTLV-1) Lecture Twenty-five Human Herpesvirus and Cancer virus(HPV) 1. Herpesviruses (1)The properties of Herpesviruses (2) Herpes simplex viruses and infections(latent infection) (3) Varicella-Zoster virus and infections(latent infection) (4)Cytomegalovirus and infections(congenital infection) (5)Epstein-Barr virus (6)Kaposis sarcoma-associated herpesvirus (7) Other human herpesvirus infection 2. Human cancerⅤirus (1)General Features of viral carcinogenesis (2) Papillomavirus and infections (3) Polyomaviruses and infections (4)Other human cancer viruses
10 / 11 2. Hepatitis virus C (1) The main properties of Hepatitis C virus (genome) (2) Hepatitis C virus infection （chronic infection） (3) Diagnosis, treatment, prevention & control of hepatitis C infections 3. Hepatitis virus E (1) The main properties of Hepatitis E virus (2) Hepatitis E virus infection (3) Prevention & control of hepatitis E infections Lecture Twenty-three Athropod-borne and rodent-borne viral diease 1. Togavirus and Flavivirus Encephalitis (1) The main properties of Togavirus and Flavivirus (2) Transmission cycle of Japanese B encephalitis (3) Pathogensis and clinical profiles of Togavirus and Flavivirus infections (4) Laboratory Diagnosis of Japanese B encephalitis (5) Treatment, prevention & control of Japanese B encephalitis (6) Dengue virus and dengue hemorrhagic fever (7) Other viral encephalitis (West nile fever, etc.) 2. Viral hemorrhagic fever (1) Main properties of Viral hemorrhagic fever (2) Hantavirus and hemorrhagic fever with renal syndrome and pulmonary syndrome (3) Lassa fever etc. Lecture Twenty-four Retrovirus & Human Immunodeficiency virus 1. Concept of Retrovirus 2. Major biological properties of HIV: including morphology and structure，genome and replication cycle 3. HIV Pathogenic mechanism and Immunological function 4. HIV laboratory diagnostic tests and the principles of prevention and treatment 5. Human T-cell lymphotropic virus (HTLV-1) Lecture Twenty-five Human Herpesvirus and Cancer Virus (HPV) 1. Herpesviruses (1) The properties of Herpesviruses (2) Herpes simplex viruses and infections (latent infection) (3) Varicella-Zoster virus and infections (latent infection) (4) Cytomegalovirus and infections (congenital infection) (5) Epstein-Barr virus (6) Kaposi’s sarcoma-associated herpesvirus (7) Other human herpesvirus infection 2. Human Cancer Virus (1) General Features of viral carcinogenesis (2) Papillomavirus and infections (3) Polyomaviruses and infections (4) Other human cancer viruses