Section b Microbial metabolism B2 Electron Transport The Mitochondrion outer Inner Oxidative Phosphorylation and membrane membrane B-oxiantion of Fatty Acids 电子传递,氧化磷酸化和 脂肪酸的β-氧化 intermembrane matrix基质 cristae 脊
Section B Microbial metabolism 脊 基质 B2 Electron Transport, Oxidative Phosphorylation and β-oxiantion of Fatty Acids 电子传递，氧化磷酸化和 脂肪酸的β-氧化
1. Electron Transport 1.1电子传递: There is a electron transport chain. It is used to transport electron.n the electron transport chain, a series of balanced oxidation and reduction reactions drives the movement of electrons through the carrier series from NADH to oxygen During this process energy is released and aTP is synthesized ATP ATP ATP MuM)(XaMK AH2 人 还原 细胞色素氧 氧化型 黄素蛋白辅酶Q 细胞色素
1. Electron Transport ◼ 1.1 电子传递：There is a electron transport chain. It is used to transport electron. In the electron transport chain, a series of balanced oxidation and reduction reactions drives the movement of electrons through the carrier series from NADH to oxygen. During this process energy is released and ATP is synthesized 辅酶Q 辅酶QH2 脱氢酶 脱氢酶 细胞色素氧化酶 还原型 氧化型 黄素蛋白 辅酶Q 细胞色素 H2O 1/2O2 2a 2a3 1 2 ATP 2Fe2+ 2Fe2+ 2Fe3+ 2Fe3+ 2c 2Fe2+ 2Fe3+ ATP 2b 2Fe3+ 2Fe2+ FAD FADH2 ATP NADH2 NAD A A H
Electron Transport 12电子传递的五类物质: NADH dehydrogenases. Enzymes that catalyse transfer of hydrogen atoms from reduced NAD to flavoproteins Flavoproteins. Flavin mononucleotide( mn) and flavin adenine dinucleotide AD). The flavins are reduced by accepting a hydrogen atom from NAdH and oxidized by losing an electron Electron carriers, cytochromes. Cytochromes are porphyrin containing proteins each of Which can be reduced or oxidized by the loss of a single electron Iron sulfur proteins. These are carriers of electrons with a range of reduction potentiales Quinones. These are lipid-soluble carriers that can diffuse through membranes carrying electrons from iron-sulfur proteins to cytochromes
1. Electron Transport ◼ 1.2 电子传递的五类物质： ◼ NADH dehydrogenases.Enzymes that catalyse transfer of hydrogen atoms from reduced NAD to flavoproteins. ◼ Flavoproteins.Flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). The flavins are reduced by accepting a hydrogen atom from NADH and oxidized by losing an electron. ◼ Electron carriers, cytochromes.Cytochromes are porphyrin containing proteins each of which can be reduced or oxidized by the loss of a single electron. ◼ Iron sulfur proteins.These are carriers of electrons with a range of reduction potentiales. ◼ Quinones.These are lipid-soluble carriers that can diffuse through membranes carrying electrons from iron-sulfur proteins to cytochromes
(1)烟酰胺脱氢酶类 以NAD或NADP+为辅酶,目前已知者达20多种。此类酶催化脱氢时,其辅酶NAD'或 NA先和酶的活性部位结合,然后再脱下来。它与代谢物脱下的氢结合而还原成NA或 NAPt。当有受H体存在时,NADH或 NADPH的H可被脱下而氧化为NAD或NADP+2 NAD*+2H(2H*+2e =NADH+H+ NADP*+ 2H(2H*+ 2 e).NADPH+H conh s CONH +2Ht+2e +F↑ NAD NADH
Flavoprotein(黄素蛋白):黄素蛋白是一种FAD为辐基的蛋白,他的作用 是依靠FAD传递氢 Quinones(醌类):具有传递氢和电子的作用 CH3O CH CH,o CH2 CH=C-CH2)nH CH 6-10 细胞色素(b、c、a):是一类以铁卟啉衍生物为辐基的蛋白质,传递电子。 CH--S-CH2' Intermembrane Proton space CH-S-CH2- membrane Cu Cytochrome c oxidase Matrix
◼ Flavoprotein（黄素蛋白）：黄素蛋白是一种FAD为辐基的蛋白，他的作用 是依靠FAD传递氢。 ◼ Quinones（醌类）：具有传递氢和电子的作用。 ◼ 细胞色素（b、c、a）：是一类以铁卟啉衍生物为辐基的蛋白质，传递电子。 O O CH3O CH3O CH3 (CH2CH C CH2)nH CH3 n=6-10
tb) Cys s Cys Protein cysS、S.SCs S-C Cys-S Cys-S S-CYs Protein
液(外制 粒体内膜基质(内悯 NADh+hi 2H 2n6 QH 图78化学滲透学说中呼吸资上氧化还原环节 可能构形图
outer membrane 2 inner membrane 》 02H20 NADH NAD matrix
outer membrane innermembrane 中 space 叶H H 22832225 synthetase inner membrane ADP+ Pi ATP Matrix
INTERMEMBRANE SPACE NADHNAD 2O2+ 2H* H20 +H 3ADP+ 3P MATRIX 3 ATP