广东医科大学：《临床生物化学及检验》课程教学资源（各章练习，含答案）第二十章 治疗药物监测

1. Enzymes were among the first biological macromolecules to be studied chemically 1.1 Much of the early history of biochemistry is the history of enzyme research. 1.1.1 Biological catalysts were first recognized in studying animal food digestion and sugar fermentation with yeast (brewing and wine making)

Assay:化验,分析,测定。 Enzyme activity: unit() is defined as the amount of enzyme which convert 1 micromole (umol) of substrate to product(s) in 1 minute under specified conditions. Specific activity: enzyme activity expressed per unit mass of protein present. (units/mg)

dissociation and reconstitution studies Abundant knoblike protruding structures were observed on the matrix side of the inner mitochondrial membrane by EM (Racker in 1960). The inside-out submitochondrial particles with the knobs\ are capable of both electron transferring and ATP synthesis

It is beyond the scope of this class to understand the following. If only one equilibration is in effect, the pH of the solution is determined by one Henderson-Hasselbalch equation

table 13-1 Some Signals to Which Cells Respond Antigens Cell surface glycoproteins/oligosaccharides Developmental signals Extracellular matrix components Growth factors Hormones Light Mechanical touch Neurotransmitters Odorants

1. Living matter is composed mostly of the lighter elements 1.1 The composition of living matter is strikingly different from that of its physical environment(1810s) 1.2 The elements found in living organisms also exist in nature(especially in seawater and atmosphere)

1. Biomembranes are organized, sheetlike assemblies consisting mainly of proteins and lipids 1.1 The functions carried out by membranes are diverse and indispensable for life. 1.1.1 Biomembranes give cells their individuality by separating them from the environment

1.1 The Definition of Metabolism The highly integrated network of chemical transformations. Degradation (decreasing order, thus energy releasing) to provide energy, catabolism; Synthesis (increasing order, thus energy consuming) to provide building materials, anabolism

1. Cells need energy to do all their work To generate and maintain its highly ordered structure (biosynthesis of macromolecules). To generate all kinds of movement. To generate concentration and electrical gradients