GAS LIQUID CHROMATOGRAPHY Principles Partition of molecules between gas (mobile phase) and liquid (stationary phase)
GAS LIQUID CHROMATOGRAPHY Principles Partition of molecules between gas (mobile phase) and liquid (stationary phase)
Most Common Stationary Phases 1. Separation of mixture of polar compounds Carbowax 20M (polyethylene glycol) 2. Separation of mixtures of non-polar compounds OV101 or SE-30 (polymer of methylsilicone) 3. Methylester of fatty acids DEGS (diethylene glycol succinate)
Most Common Stationary Phases 1. Separation of mixture of polar compounds Carbowax 20M (polyethylene glycol) 2. Separation of mixtures of non-polar compounds OV101 or SE-30 (polymer of methylsilicone) 3. Methylester of fatty acids DEGS (diethylene glycol succinate)
Filters/Traps Air Hydrogen Gas Carrier Column Gas Chromatography ⚫ gas system ⚫ inlet ⚫ column ⚫ detector ⚫ data system Data system Syringe/Sampler Inlets Detectors Regulators H RESET
Filters/Traps Air Hydrogen Gas Carrier Column Gas Chromatography ⚫ gas system ⚫ inlet ⚫ column ⚫ detector ⚫ data system Data system Syringe/Sampler Inlets Detectors Regulators H RESET
Schematic Diagram of Gas Chromatography
Schematic Diagram of Gas Chromatography
Schematic Diagram of Gas Chromatography
Schematic Diagram of Gas Chromatography
DETECTORS Flame Ionization Detector (Nanogram - ng) High temperature of hydrogen flame (H2 +O2 + N2 ) ionizes compounds eluted from column into flame. The ions collected on collector or electrode and were recorded on recorder due to electric current
DETECTORS Flame Ionization Detector (Nanogram - ng) High temperature of hydrogen flame (H2 +O2 + N2 ) ionizes compounds eluted from column into flame. The ions collected on collector or electrode and were recorded on recorder due to electric current
Exhaust Chimney Igniter Hydrogen Inlet Column Effluent Polarizing Electrode Collector Electrode Schematic Diagram of Flame Ionization Detector
Exhaust Chimney Igniter Hydrogen Inlet Column Effluent Polarizing Electrode Collector Electrode Schematic Diagram of Flame Ionization Detector
Schematic Diagram of Flame Ionization Detector Collector Jet Flame Detector electronics - 220 volts Column Chassis ground Signal output
Schematic Diagram of Flame Ionization Detector Collector Jet Flame Detector electronics - 220 volts Column Chassis ground Signal output
Thermal Conductivity Detector Measures the changes of thermal conductivity due to the sample (mg). Sample can be recovered
Thermal Conductivity Detector Measures the changes of thermal conductivity due to the sample (mg). Sample can be recovered
Thermal Conductivity Detector Principal: The thermal balance of a heated filament Electrical power is converted to heat in a resistant filament and the temperature will climb until heat power loss form the filament equals the electrical power input. The filament may loose heat by radiation to a cooler surface and by conduction to the molecules coming into contact with it
Thermal Conductivity Detector Principal: The thermal balance of a heated filament Electrical power is converted to heat in a resistant filament and the temperature will climb until heat power loss form the filament equals the electrical power input. The filament may loose heat by radiation to a cooler surface and by conduction to the molecules coming into contact with it