BIOCHEMISTRYANDMOLECULARBIOLOGYChapter 29Metabolism of NucleotidesDepartment of Biochemistryand Molecular Biology,MedicineSchool
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Chapter 29 Metabolism of Nucleotides
山BIOCHEMISTRYANDMOLECULARBIOLOGY1. overviewDigestion andabsorptionofnucleic acidNucleic acid and proteinacid of stomach>Nucleic acid(RNA and DNA)proteinNuclease of pancreaticnucleotideNucleotide enzymephosphatenucleoside Nucleoside enzymebase→ riboseDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY ⚫ Digestion and absorption of nucleic acid Nucleic acid and protein protein Nucleic acid(RNA and DNA） acid of stomach nucleotide Nuclease of pancreatic nucleoside phosphate Nucleotide enzyme base ribose Nucleoside enzyme 1. overview
BIOCHEMISTRYANDMOLECULARBIOLOGYThe function of nucleotideThe material of synthesis nucleic acidPlay an role as energy currencyNucleotide are important regulatory compounds formany of the pathways of intermediary metabolismThe component of coenzymeServe as carriers of activated intermediates in thesynthesis of some carbohydrates, lipids, and proteins.Department of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY ⚫ The material of synthesis nucleic acid ⚫ Play an role as energy currency ⚫ Nucleotide are important regulatory compounds for many of the pathways of intermediary metabolism ⚫ The component of coenzyme ⚫ Serve as carriers of activated intermediates in the synthesis of some carbohydrates, lipids, and proteins. The function of nucleotide
ABIOCHEMISTRYANDMOLECULARBIOLOGYNicotinamide adenine dinucleotide. NAD+NH2AMPO-P-O-H,CCONHOHOH"O-P-O-H,C=OOHOHDepartment of Biochemistryand Molecular Biology,MedicineSchool
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY AMP Nicotinamide adenine dinucleotide, NAD+
ABIOCHEMISTRYANDMOLECULARBIOLOGYI. Metabolism of Purine NucleotidesThestructureofpurinenucleotideNH福HNR5'-PR5'-PAMPGMPDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY ⚫The structure of purine nucleotide AMP GMP II. Metabolism of Purine Nucleotides
BIOCHEMISTRYANDMOLECULARBIOLOGYSynthesis pathway of purine nucleotideDe novo synthesis pathwaySalvage synthesis pathwayDepartment of Biochemistryand Molecular Biology,MedicineSchool
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY De novo synthesis pathway Salvage synthesis pathway Synthesis pathway of purine nucleotide
BIOCHEMISTRYANDMOLECULARBIOLOGYA. De novo purine nucleotide synthesis·DefinitionThe atoms of the purine ring are contributed by anumber of compounds, including amino acid(asparticacid, glycine, and glutamine), CO2, and derivatives oftetrahydrofolate.·SynthesisorganThe major organ for synthesis purine nucleotide is liver,next organ is intestine and thymus gland, but brain and bonemarrow can not use de novo to synthesize purine nucleotide.Department of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY The atoms of the purine ring are contributed by a number of compounds, including amino acid(aspartic acid, glycine, and glutamine), CO2 , and derivatives of tetrahydrofolate. The major organ for synthesis purine nucleotide is liver， next organ is intestine and thymus gland，but brain and bone marrow can not use de novo to synthesize purine nucleotide. •Definition •Synthesis organ A. De novo purine nucleotide synthesis
ABIOCHEMISTRYANDMOLECULARBIOLOGYSources of the individual atoms in thepurine ringCO2Glycine1CNAspartateNiCN10-Formy!-CBtetrahydrofolateN10-Formyl-（Onecarbonunit)CNgtetrahydrofolateN3(One carbon unit)1→GlutamineDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY CO2 Aspartate N10-Formyltetrahydrofolate （One carbon unit) Glycine N10 -Formyltetrahydrofolate （One carbon unit） Glutamine Sources of the individual atoms in the purine ring
沙BIOCHEMISTRYANDMOLECULARBIOLOGYMaterial GlycineGlycine in the middleCO2The top is CO2 AspartateLeft up is Aspartate GlutamineGlutamine and oneOne carbon unitcarbon unit in the two side5'-Phosphate riboseDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Material • Glycine • CO2 • Aspartate • Glutamine • One carbon unit • 5’-Phosphate ribose Glycine in the middle The top is CO2 Left up is Aspartate Glutamine and one carbon unit in the two side
BIOCHEMISTRYANDMOLECULARBIOLOGYPathway1. Synthesis of IMP2. Synthesis of AMP and GMPDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY 1. Synthesis of IMP 2. Synthesis of AMP and GMP Pathway