BIOCHEMISTRYANDMOLECULARBIOLOGYChapter 18phospholipid metabolismDepartment of Biochemistryand MolecularBiology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Chapter 18 phospholipid metabolism
BIOCHEMISTRYANDMOLECULARBIOLOGYOutline*Structure of phospholipid*Synthesis of phospholipids*Function of phospholipids*Degradation of phospholipidsDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY Outline *Structure of phospholipid *Synthesis of phospholipids *Function of phospholipids *Degradation of phospholipids
BIOCHEMISTRYANDMOLECULARBIOLOGY1.Structure of phospholipidThere are two classes of phospholipids:those that have glycerol as a backbone and those thatcontain sphingosineDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY There are two classes of phospholipids: those that have glycerol as a backbone and those that contain sphingosine. 1. Structure of phospholipid
ABIOCHEMISTRYANDMOLECULARBIOLOGY00CH2-0-C-R11IR2-C-0-CHOCH2-0-P-0-XOHThe structure of phospholipids that have glycerolbackboneDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY The structure of phospholipids that have glycerol backbone
BIOCHEMISTRYANDMOLECULARBIOLOGY2.SynthesisofphospholipidsThese synthetic pathways involve the phosphorylationof choline or ethanolamine, followed by conversion tothe activated form, CDP-choline or CDP-ethanolamine. Finally, choline-phosphate or ethanolamine-phosphate is transferred from the nucleotide (leavingCMP) to a molecule of diacylglycerol Department of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY 2. Synthesis of phospholipids These synthetic pathways involve the phosphorylation of choline or ethanolamine, followed by conversion to the activated form, CDP-choline or CDP-ethanolamine. Finally, choline-phosphate or ethanolaminephosphate is transferred from the nucleotide (leaving CMP) to a molecule of diacylglycerol
ANDMOLECULARBIOLOGY0川0CH2-O-C-R11R2-C-OO-CHCH2-O-P-0-XOHX is -CH2CH2N+(CH3)3It's(lecithine)phosphatidylcholineX is -CH2CH2NH+3 It's(cephalin)phosphatidylethanolamineDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY X is -CH2CH2N+ (CH3 )3 , It’s phosphatidylcholine（lecithine） X is -CH2CH2NH+ 3, It’s phosphatidylethanolamine（cephalin）
BIOCHEMISTRYANDMOLECULARBIOLOGY>Synthesis of site:all of kinds cells can synthesize except muture erythrocyteshowever, liver、 kidney and intestinal ease to synthesizematerial and cofactor:diacylglycerol, phosphate, choline, serine, ethanolamineATP, CTPDepartment of Biochemistry and Molecular Biology,Medicine School
Department of Biochemistry and Molecular Biology, Medicine School BIOCHEMISTRY AND MOLECULAR BIOLOGY ➢Synthesis of site： all of kinds cells can synthesize except muture erythrocytes ，however, liver、kidney and intestinal ease to synthesize ➢ material and cofactor： diacylglycerol, phosphate, choline, serine, ethanolamine, ATP, CTP
Synthesispathway:HOCH2CHCOOHserineCO2NH2ethanolamineHOCH2CH2 NH2 N(CH3)33SAMHOCH2CH2cholineATPATPADPADP?OCH2CH2 NH2?oCH2CH2 N(CHB)3PhosphateethanolaminePhosphate choline/ CTP/ CTPCTP:choline phosphateCTP:ethanolaminecytidine transferasephosphate cytidinetransferasePPiPPiCDP-0CHCH2 N(CHB)3CDP-OCH2CH2 NH2CDP-cholineCDP-ethanolamine
Synthesis pathway： ethanolamine CTP：ethanolamine phosphate cytidine transferase HOCH2CHCOOH NH2 HOCH2CH2 NH2 HOCH2CH2 N(CH3)3 + OP OCH2CH2 NH2 OCH2CH2 N(CH3)3 O + P CDP-OCH2CH2 NH2 OCH2CH2 N(CH3)3 + CDP- CO2 3SAM ATP ATP ADP ADP CTP：choline phosphate cytidine transferase CTP CTP CDP-ethanolamine CDP-choline choline serine Phosphate ethanolamine Phosphate choline PPi PPi
A. Diacylglycerol synthesis pathway:acyltransferaseglucose+ 3-phosphatePhosphatidic acid2RCOCoA2CoAPi1, 2-diacylglycerolCDP-cholineCDP-ethanolamineAcyl CoACMP'CMPI'CoAPhosphatidylcholinePhosphatidyltriacylglycerolethanolamine(lecithine)(cephalin)
acyltransferase 2RCOCoA 2CoA 1，2-diacylglycerol Phosphatidylcholine （lecithine） Phosphatidyl ethanolamine （cephalin） triacylglycerol CDP-ethanolamine CDP-choline Acyl CoA glucose 3-phosphate Phosphatidic acid A. Diacylglycerol synthesis pathway： CMP CMP CoA Pi
B.CDP-diacylglycerol synthesis pathway:acyltransferasephosphatidicglucose 3-phosphateacid+glycerol2CoA2RCOCoACTPCytidinetransferasePPiCDP-diacylglycerolphosphatidylglycerolinositolserineCMPCMPCMPcardiolipinphosphatidylserinephosphatidylinositol
acyltransferase 2RCOCoA 2CoA phosphatidylinositol phosphatidylserine cardiolipin inositol CMP CMP CMP serine phosphatidylglycerol CDP-diacylglycerol Cytidine transferase CTP PPi glucose 3-phosphate glycerol phosphatidic acid B.CDP-diacylglycerol synthesis pathway：