Impairment of mitochondrial complex activity associated with ischemia and reperfusion Involvement of Reactive Oxygen Species (ROS)and Cardiolipin Yao Hui
Impairment of mitochondrial complex activity associated with ischemia and reperfusion Involvement of Reactive Oxygen Species (ROS) and Cardiolipin Yao Hui
Mitochondrial structure It is well known that mitochondrion is the power source of cells and its function is dependent on the activities of respiratory chain complexes
It is well known that mitochondrion is the power source of cells and its function is dependent on the activities of respiratory chain complexes. Mitochondrial structure
Intermembrane space H Inner mitochondrial membrane NAD NADH +H' Fumarate Succinate 02+2H*H0 Matrix Mitochondrial Respiratory Chain Complexes
Mitochondrial Respiratory Chain Complexes
In cardiac myocytes mitochondria are abundant constituting 20%~40%of celluler volume.Their primary function is to supply ATP through oxidative phosphorylation,which could meet high energy demands of the beating heart
In cardiac myocytes mitochondria are abundant constituting 20% ~40% of celluler volume. Their primary function is to supply ATP through oxidative phosphorylation, which could meet high energy demands of the beating heart
Now many studies of several labs have shown when the hearts encountered ischemia or reperfusion challenge,the activities of mitochondrial complexes were decreased
Now many studies of several labs have shown when the hearts encountered ischemia or reperfusion challenge, the activities of mitochondrial complexes were decreased
Activities of complex I.III in mitochondria isolated from control,ischemic and reperfused rat hearts 800 0 250 50 Control Reperfus
Activities of complex I、III in mitochondria isolated from control ,ischemic and reperfused rat hearts
Kinetic parameters of complex IV in mitochondria isolated from control,ischemic and reperfused rat hearts Cytochrome c oxidase V (natoms Heart Kin(M) O/min/mg protein) Decrement Control 15.6±0.71 3900±71 Ischemic 16.1±0.78 2927±63$ 25 Reperfused 15.7±0.57 1915±96 51
Kinetic parameters of complex IV in mitochondria isolated from control ,ischemic and reperfused rat hearts
RO8 skgnallng protecdon llplda DNA protolns It is generally acknowledged that ROS plays an important role in the cell injury associated with cardiac ischemia and reperfusion
It is generally acknowledged that ROS plays an important role in the cell injury associated with cardiac ischemia and reperfusion
Relationship between ROS and mitochondrial complexes CuSOD.GPx.catalase Glutathione RESPIRATORY COMPLEXES 0 线AE peptides Matrix AIF caspase Inner membrane Activation of caspases Outer membrane Cytosol APOPTOTIC CELL
Relationship between ROS and mitochondrial complexes
Peroxidation of membrane lipid components has been hypothesized to be a major mechanism of ROS toxicity,resulting in impairment of membrane function.Due to its unique membrane composition,the mitochondrion is especially sensitive to lipid peroxidation
Peroxidation of membrane lipid components has been hypothesized to be a major mechanism of ROS toxicity, resulting in impairment of membrane function. Due to its unique membrane composition, the mitochondrion is especially sensitive to lipid peroxidation