Mitochondrial Membrane Potential (MMP)
Mitochondrial Membrane Potential (MMP)
Contents Definition of MMP Formation of MMP Measurement/estimation of MMP Role of MMP in function of both normal and pathophysiology
Contents ⚫Definition of MMP ⚫Formation of MMP ⚫Measurement/estimation of MMP Role of MMP in function of both normal and pathophysiology
Definition of MMP ●Formation of MMP Measurement/estimation of MMP Role of MMP in mitochondrial function of both normal and pathophysiology
⚫Definition of MMP ⚫Formation of MMP ⚫Measurement/estimation of MMP Role of MMP in mitochondrial function of both normal and pathophysiology
Inner -Membrane Outer Membrane The miotchondrial membrane potential is a highly negative (~-180mV Cristae potential across the inner membrane of mitochondria
The miotchondrial membrane potential is a highly negative (~ -180mV) potential across the inner membrane of mitochondria
o Definition of MMP ●Formation of MMP Measurement/estimation of MMP Role of MMP in mitochondrial function of both normal and pathophysiology
⚫Definition of MMP ⚫Formation of MMP ⚫Measurement/estimation of MMP Role of MMP in mitochondrial function of both normal and pathophysiology
intermembrane space Inner H Membrane ETS UCP ATP/ADP MMP ATP-ase Outer translocase -Membrane .m Cristae Matrix matrix Sketch map showing the structure of mitochondria (on the left and principle of generation of MMP in mitochondria.Electrontransport system(ETS)pumps protons across the inner mitochondrial membrane (im)and thus generates membrane potential (MMP)
Sketch map showing the structure of mitochondria (on the left ) and principle of generation of MMP in mitochondria. Electron transport system (ETS) pumps protons across the inner mitochondrial membrane (i.m) and thus generates membrane potential (MMP)
Definition of MMP ●Formation of MMP oMeasurement/estimation of MMP Role of MMP in mitochondrial function of both normal and pathophysiology
⚫Definition of MMP ⚫Formation of MMP ⚫Measurement/estimation of MMP Role of MMP in mitochondrial function of both normal and pathophysiology
Principle of measurement of MMP oDirectly measurement using electrode oIndirectly measurement using fluorescent indicators
Principle of measurement of MMP ⚫Directly measurement using electrode ⚫Indirectly measurement using fluorescent indicators
oDirectly measurement using electrode oIndirectly measurement using fluorescent indicators
⚫Directly measurement using electrode ⚫Indirectly measurement using fluorescent indicators
p4, [Ca2=105 mM 160 80 80160 105m 100rM CwrM与