Model for Global Cerebral Ischemia in the Rat 阙
Model for Global Cerebral Ischemia in the Rat 梁
Blood supply of the brain
Blood supply of the brain
Types of model Two-vessel occlusion model Four-vessel occlusion model
Types of model ◼ Two-vessel occlusion model ◼ Four-vessel occlusion model
How to make (1) Four-vessel occlusion model Both vertebral arteries are cauterized Both common carotid arteries are clamped Duration of ischemia:15-30 min
How to make (1) ◼ Four-vessel occlusion model ◼ Both vertebral arteries are cauterized ◼ Both common carotid arteries are clamped ◼ Duration of ischemia: 15–30 min
How to make(2) Two-vessel occlusion model By clamping bilateral common carotid arteries and bleeding hypotension to 45-50 mm Hg Reperfusion:by re-infusing the shed blood and releasing the clamps Duration of ischemia:5-15 min
How to make (2) ◼ Two-vessel occlusion model ◼ By clamping bilateral common carotid arteries and bleeding hypotension to 45–50 mm Hg ◼ Reperfusion: by re-infusing the shed blood and releasing the clamps ◼ Duration of ischemia: 5–15 min
How to judge In a coma within 30-60 s ■Righting reflex lost Unresponsive to somatosensory stimulation, with pupils fully dilated ■An isoelectric EEG
How to judge ◼ In a coma within 30-60 s ◼ Righting reflex lost ◼ Unresponsive to somatosensory stimulation, with pupils fully dilated ◼ An isoelectric EEG
What is happened Sham
What is happened
P0.D01 sndureoddryu P-0.102 3 2, Sham 2030 40 (min) Ischemia duration
B
A B