Chapter 6 微生物的生长与控制 Microbial Growth and Control AHNU
CCTV FUTURE ADVERTISING COMPANY IS CCTV TO EXOLORE
Contents 1 2 3 4 Main Factors Impact Microbial Growth Detection Method Growth Curve Cultivation of Microbes 5 Control the Harmful Microbes
1 Detection Method ● Measuring the biomass (生物量) (1) Direct method: dry mass weighing (2) Indirect method: turbidimetry; Physiological index
• ● Countering cell number • (1) Direct method: • Vital staining • (2) Indirect method: • Plate colony counting for: • Anaerobe (discuss late) • aerobic bacteria
• Pour plate technique/倾注平板法
2.1 Synchronous growth/ 同步生长 (1) Environmental condition induction method (2) Mechanical screening/机械筛选法 2 Growth Curve
Helmstetter-Cummigs 膜洗脱法
• 2.2 Typical growth curve for single-celled microorganisms
• 2.2.1 Lag phase • When microorganisms are introduced into fresh culture medium, usually no immediate increase in cell number occurs. This period is called the lag phase