Networks of biological Signaling pathways 信号传递网络 康滷岐高方远马欣荣
Networks of Biological Signaling Pathways 信号传递网络 康海岐 高方远 马欣荣
、生物体内的信号传递 o 1. The sense of signal transduction intercelluar information exchange regulation of metobolism, on body level o 2. Type of signals ● neuroregulation: neurotransmitter(z酰胆碱胺类E sce ell 氨基酸调节肽类等), neuroregulator chemical signalS: CAMP, Calt, hormone 3. Mechanisms 3.1 32 E reaction(±p) 3.3 E activity 3.4 pr. degradation 3.5 intracelluar messager 3.6 seconder messager
一、生物体内的信号传递 ⚫ 1. The sense of signal transduction： ⚫ intercelluar information exchange,regulation of metobolism, on body level ⚫ 2. Type of signals： ⚫ neuroregulation: neurotransmitter(乙酰胆碱,胺类 ⚫ 氨基酸,调节肽类等),neuroregulator ⚫ chemical signals：cAMP, Ca2+ , hormone， ⚫ 3. Mechanisms: ⚫ 3.1 pr. ←→pr., ⚫ 3.2 E reaction(±p ) ⚫ 3.3 E activity ⚫ 3.4 pr. degradation ⚫ 3.5 intracelluar messager ⚫ 3.6 seconder messager ⚫ E cell
生物体内的信号传递 o 4 Signaling pathways: 4.1Ca2+ 42 CAMP 4.3 tyrosine kinase: EGFR, insulinr 4.4 other pr. kinase cascade: PKC, PKA, PKG 4.5 intracelluar protease cascade o Signal transmission occur 1. Pr.pr. Interaction i1. Enzymatic reaction: tp ll1. Pr Degradation 1111. Production of intracellular messager
一、生物体内的信号传递 ⚫ 4. Signaling pathways： ⚫ 4.1 Ca2+ ⚫ 4.2 cAMP ⚫ 4.3 tyrosine kinase: EGFR,insulinR ⚫ 4.4 other pr. kinase cascade:PKC,PKA,PKG ⚫ 4.5 intracelluar protease cascade ⚫ Signal transmission occur: ⚫ i. Pr.—pr. Interaction ⚫ ii. Enzymatic reaction: ±p ⚫ iii. Pr. Degradation ⚫ iiii. Production of intracellular messager
生物体内的信号传递 o 5. cytoplasm membrane receptor 5.1 neurotransmitter-dependention channel (依赖神经递质的离子通道) nAChR(烟碱型乙酰胆碱受体) GABA(y氨基丁酸) GlyR(甘氨酸受体) o 5.2 receptor connecting to signal transduction protein (G,N protein-second messenger -activate E) mAChR(毒蕈碱型乙酰胆碱受体) adrenergic a-β- receptor(肾上腺素能a--受体) 5.3 growth factor receptor( tyrosine kinase activity) PDGFR(血小板衍生的生长因子受体), EGFR(表皮生长因子受体, insulin r(胰岛素受体)
一、生物体内的信号传递 ⚫ 5. cytoplasm membrane receptor： ⚫ 5.1 neurotransmitter-dependention channel ⚫ (依赖神经递质的离子通道)： ⚫ nAChR(烟碱型乙酰胆碱受体) ⚫ GABA(γ -氨基丁酸) ⚫ GlyR(甘氨酸受体) ⚫ 5.2 receptor connecting to signal transduction protein ⚫ (G,N protein →second messenger →activate E.): ⚫ mAChR(毒蕈碱型乙酰胆碱受体) ⚫ adrenergic α-,β-receptor (肾上腺素能 α-,β-受体) ⚫ 5.3 growth factor receptor(tyrosine kinase activity)： ⚫ PDGFR(血小板衍生的生长因子受体)， ⚫ EGFR(表皮生长因子受体)，insulin R(胰岛素受体)
Peptide signaling in Plants PNAS. NoV 6. 2001. voL.98 no 23 In plants, only a few peptide have been identified that act as signaling molecules e In contrast, signaling peptides are major players in all aspects of the life cycle in animals and yeast suggests that signaling mechanisms across the eukaryotic kingdom are fundamentally different
Peptide Signaling in Plants PNAS, Nov. 6, 2001, vol.98 no. 23 • In plants, only a few peptide have been identified that act as signaling molecules. • In contrast, signaling peptides are major players in all aspects of the life cycle in animals and yeast. • suggests that signaling mechanisms across the eukaryotic kingdom are fundamentally different
1.目前有关植物中信号肽的研究主要基于以下5种: 番茄 systemin PSK ENOD40cLV3SCR 18 aa 10-13a72-75aa、53-55aa 2.最近分离到另外3种活性信号肽 RALF: rapid alkal inization factor 5 kd Tobacco systemin: Tob sys I, Tob sys II
1. 目前有关植物中信号肽的研究主要基于以下5种： 番茄systemin PSK ENOD40 CLV3 SCR 18 aa 10-13 aa 72-75 aa 53-55 aa 2. 最近分离到另外3种活性信号肽： RALF: rapid alkalinization factor, 5 kd; Tobacco systemin: Tob sys I, Tob sys II
3.功能: 1) tomato systemin:由食草动物损伤后引起的系统 损伤反应( a system ic wounding response) °在悬浮培养细胞中可以激活促细胞分裂蛋白激酶 Lmitogen-activated protein( MAp)kinase] ●并诱导培养基地碱化( alkal inization) ●诱导蛋白酶抑制蛋白编码基因的表达( induce expression of prote inase-inhibitor prote in-encod ing genes)
1）tomato systemin: 由食草动物损伤后引起的系统 损伤反应( a systemic wounding response) • 在悬浮培养细胞中可以激活促细胞分裂蛋白激酶 [mitogen-activated protein(MAP) kinase] • 并诱导培养基地碱化(alkalinization) • 诱导蛋白酶抑制蛋白编码基因的表达(induce expression of proteinase-inhibitor protein-encoding genes) 3. 功能：
2) tobacco systemin Tob I and Tob I 激活 maP kinase,但不诱导蛋白酶抑制蛋白编码 基因的表达 3)RALF (rapid alkal inizaton factor) °激活 maP kinase,但不诱导蛋白酶抑制蛋白编码 基因的表达; 快速引起 med ium碱化
2）tobacco systemin Tob I and Tob II: 激活 MAP kinase，但不诱导蛋白酶抑制蛋白编码 基因的表达 3）RALF (rapid alkalinizaton factor): • 激活 MAP kinase，但不诱导蛋白酶抑制蛋白编码 基因的表达; • 快速引起 medium 碱化
4.信号调控网络 From the followings support the idea that peptide and nonpeptide hormone-activated signaling cascades are linked in plants as they are in animals: ●植物生长素类似5一羟色胺,乙烯类似一氧化碳, 油菜素类固醇是类固醇,茉莉酮酸与前列腺素相关; Systemin-induced wound response is regulated through the octadecanoid pathway involving jasmonic acid
From the followings support the idea that peptide and nonpeptide hormone-activated signaling cascades are linked in plants as they are in animals: • 植物生长素类似5－羟色胺，乙烯类似一氧化碳， 油菜素类固醇是类固醇，茉莉酮酸与前列腺素相关； • Systemin-induced wound response is regulated through the octadecanoid pathway, involving jasmonic acid; 4. 信号调控网络
PSK-induced cell proliferation requires the hormones auxin or cytokinin; o Some of the developmental distortions in roots induced on addition of ralf are reminiscent of impaired nonpeptide hormone-controlled processes 因此,揭开两种信号 cascades之间关系,将是非常 有趣的事
• PSK-induced cell proliferation requires the hormones auxin or cytokinin; • Some of the developmental distortions in roots induced on addition of RALF are reminiscent of impaired nonpeptide hormone-controlled processes. 因此，揭开两种信号cascades之间关系，将是非常 有趣的事