stress A Z EIMER Tau Pathology Alzheimer's Disease 11307110112 xiao zitian
11307110112 xiao zitian Tau Pathology & stress
Key words GCs(glucocorticoids (2) TAU hyperphosphorylation 3 AB( Amyloid阝)
Key words: 1 GCs (glucocorticoids) 2 TAU hyperphosphorylation 3 Aβ (Amyloid β)
Method material Animal Stress 》 Infusion Behavioral Immunol model test Daily 1. Spatial reference Chronic stressors Age: 14 months Intracerebroventri memory(tested by 1. hyperon Morris water 1. Western cular infusions 2.overcrowding(1 h) ( solubilized maze blot analysis Standard 3. confined environment Aβ140 or vehicle) 2. Assessment of environmental (30mn reversal 2. immunocyto conditions 4. vibrating/rocking Sg: subcutaneous learning(collected chemistr platform (1 h) GC injections video tracking system)
Method & Material 1.Western blot analysis 2.immunocyto chemistry Immunoblot 1.Spatial reference memory(tested by Morris water maze) 2.Assessment of reversal learning(collected by video tracking system) Behavioral test Intracerebroventricular infusions (solubilized A β 1-40 or vehicle) Sg: subcutaneous GC injections Infusion Daily Chronic stressors: 1.hypertonic saline; 2.overcrowding(1 h); 3.confined environment (30 min); 4.vibrating/rocking platform (1 h) Stress model Age:14 months Standard environmental conditions Animal
Results Discussion e Kinase expression after stress f Kinase expression after GC FC PFC 200 g 100 ON itrl CON (the caks activator), pJNK, and ca in the hippocampus and PFC Sotiropoulos /, Catania C, Pinto L G, et al. Stress acts cumulatively to precipitate Alzheimer's disease-like tau pathology and cognitive deficits(J. The Journal of Neuroscience, 2011, 31(21): 7840-7847
Results & Discussion 1.induce ：chronic stress & abnormal hyperphosphorylation 2.overexpression: stress and GCs increased the expression of a number of kinases in the hippocampus and PFC of animals 3.activator: stress and/or GC activated pERK1/2, pGSK3, p35 (the cdk5 activator),pJNK, and CaMKII in the hippocampus and PFC Sotiropoulos I, Catania C, Pinto L G, et al. Stress acts cumulatively to precipitate Alzheimer's disease-like tau pathology and cognitive deficits[J]. The Journal of Neuroscience, 2011, 31(21): 7840-7847