BIOSAFETY An Introduction
BIOSAFETY An Introduction
Introduction Development of Biosafety Practices 1941- Meyer and Eddie 74 lab associated brucellosis infections in US 1949- Sulkin and pike 222 viral infections(21 fatal Only 27% related to known accidents
Introduction Development of Biosafety Practices 1941 - Meyer and Eddie 74 lab associated brucellosis infections in US 1949 - Sulkin and Pike 222 viral infections (21 fatal) Only 27% related to known accidents
Brucellosis is an infectious disease caused by the bacteria of the genus Brucella These bacteria are primarily passed among animals, and they cause disease in many different vertebrates Various Brucella species affect sheep, goats, cattle, deer, elk, pigs, dogs, and several other animals Humans become infected by coming in contact with animals or animal products that are contaminated with these bacteria
Brucellosis is an infectious disease caused by the bacteria of the genus Brucella. These bacteria are primarily passed among animals, and they cause disease in many different vertebrates. Various Brucella species affect sheep, goats, cattle, deer, elk, pigs, dogs, and several other animals. Humans become infected by coming in contact with animals or animal products that are contaminated with these bacteria
Major disease outbreaks worldwide Legionnaire's Disease Multidrug res istant Salmonella Cryptosporidiosis hon-O157 BSE -yohe E.colio157 CJD Lyme Borreliosis West Nile virus Diphtheri TReston virus Influenza A(H5N1) Lassa fevel Yellow fey. Nipah virus a0139 ○ Reston virus Venezuelan Buruli ulcer RVFNHF Equine Encephalitis Ebola ONo'nyong-nyong haemhor hagic fever hemorrh fever fe ver Dengue Human haemorrhagic Mon keypo: Cholera fever Cholera Equine Ross River morbillivirus Ir Hendra vir
Cryptosporidiosis Reston virus Venezuelan Equine Encephalitis Dengue haemhorrhagic fever Cholera E.coli O157 Typhoid Diphtheria Echinococcosis Lassa fever Yellow fever Ebola haemorrhagic fever O’nyong-nyong fever Human Monkeypox Cholera 0139 Dengue haemhorrhagic fever Influenza A(H5N1) Cholera RVF/VHF nvCJD Ross River virus Equine morbillivirus Hendra virus BSE Multidrug resistant Salmonella E.coli non-O157 West Nile Virus Malaria Nipah Virus Reston Virus Legionnaire’s Disease Buruli ulcer E.coli O157 Lyme Borreliosis West Nile Fever Major disease outbreaks worldwide
Classification of biological samples biological samples are divided into o 1. Infectious substances o 2. Diagnostic specimens
Classification of biological samples biological samples are divided into ⚫ 1. Infectious substances ⚫ 2. Diagnostic specimens
Why Biosafety Practices? Protection: ● workers products ●co- Workers o lab support personnel ● environment
Why Biosafety Practices? Protection: ⚫ workers ⚫ “products” ⚫ co-workers ⚫ lab support personnel ⚫ environment
Biosafety Biological materials ◆ Collection ◆ Transport ◆ Manipulation
Biosafety Biological materials ◆Collection ◆Transport ◆Manipulation
Collection, transport &e manipulation Any biological sample may be infectious and therefore presents a hazard to any person ◆ during collection ◆ during transport o during opening and proceeding
Collection, transport & manipulation Any biological sample may be infectious and therefore presents a hazard to any person ◆ during collection ◆ during transport ◆ during opening and proceeding
Sample collection ◆ by patients aee粪便 urine sputum 唾液,痰 ◆ by medical staff >>blood sternal puncture 胸骨的 aspiration liquids pus 吸出 脓
Sample Collection ◆by patients »faeces urine sputum ◆by medical staff »blood sternal puncture aspiration liquids pus 粪便 唾液, 痰 脓 胸骨的 吸出
Precautions during sample collection Protect collector, colleague, staff o wear gloves, coat,(mask, glasses) . VHF: double gloves, filer-masks, boots VHF=(viral hemorrhagic fever) dispose needles in special containers, without re capping, disinfection(sodium hypochlorite 2.5%) incineration o clean working surfaces(hypochlorite) o decontaminate material(hypochlorite 10%)
Precautions during sample collection Protect collector, colleague, staff... ⚫ wear gloves, coat, (mask, glasses) ◆ VHF: double gloves, filer-masks, boots VHF=(viral hemorrhagic fever) ⚫ dispose needles in special containers, without recapping, disinfection (sodium hypochlorite 2.5%), incineration ⚫ clean working surfaces (hypochlorite) ⚫ decontaminate material (hypochlorite 10%)