Chapter 4. The containment laboratory Biosafety level 3
Chapter 4. The containment laboratory – Biosafety Level 3
The containment laboratory- Biosafety level 3 is designed and provided for work with risk Group 3 microorganisms and with large volumes or high concentrations of Risk Group 2 microorganisms that pose an increased risk of aerosol spread Biosafety level 3 containment requires the strengthening of the operational and safety programmes over and above those for basic laboratories- Biosafety Levels 1 and 2
The containment laboratory – Biosafety Level 3 is designed and provided for work with Risk Group 3 microorganisms and with large volumes or high concentrations of Risk Group 2 microorganisms that pose an increased risk of aerosol spread. Biosafety Level 3 containment requires the strengthening of the operational and safety programmes over and above those for basic laboratories – Biosafety Levels 1 and 2
The guidelines given in this chapter are presented in the form of additions to those for basic laboratories- Biosafety Levels 1 and 2, which must therefore be applied before those specific for the containment laboratory -Biosafety Level 3. The major additionsand changes are in:
The guidelines given in this chapter are presented in the form of additions to those for basic laboratories – Biosafety Levels 1 and 2, which must therefore be applied before those specific for the containment laboratory – Biosafety Level 3. The major additionsand changes are in:
1. Code of practice
1. Code of practice
2. Laboratory design and facilities 3. Health and medical surveillance. laboratories in this category should be registered or listed with the national or other appropriate health authorities
2. Laboratory design and facilities 3. Health and medical surveillance. Laboratories in this category should be registered or listed with the national or other appropriate health authorities
The code of practice for basic laboratories Biosafety Levels I and 2 applies except where modified as follows
The code of practice for basic laboratories – Biosafety Levels 1 and 2 applies except where modified as follows
1. The international biohazard warning symbol and sign displayed on laboratory access doors must identify the biosafety level and the name of the laboratory supervisor who controls access, and indicate any special conditions for entry into the area, e.g. immunization
1. The international biohazard warning symbol and sign displayed on laboratory access doors must identify the biosafety level and the name of the laboratory supervisor who controls access, and indicate any special conditions for entry into the area, e.g. immunization
2. Laboratory protective clothing must be of the type with solid-front or wrap-around gowns, scrub 刷洗 suits., coverall, head covering and, where appropriate shoe covers or dedicated shoes. Front buttoned standard laboratory coats are unsuitable as are sleeves that do not fully cover the forearms Laboratory protective clothing must not be worn outside the laboratory, and it must be decontaminated before it is laundered洗烫.The removal of street clothing and change into dedicated laboratory clothing may be warranted when working with certain agents(e.g. agricultural orzoonotic agents)
2. Laboratory protective clothing must be of the type with solid-front or wrap-around gowns, scrub 刷洗suits, coveralls, head covering and, where appropriate, shoe covers or dedicated shoes. Frontbuttoned standard laboratory coats are unsuitable, as are sleeves that do not fully cover the forearms. Laboratory protective clothing must not be worn outside the laboratory, and it must be decontaminated before it is laundered洗烫. The removal of street clothing and change into dedicated laboratory clothing may be warranted when working with certain agents (e.g. agricultural orzoonotic agents)
3. Open manipulations of all potentially infectious material must be conducted within a biological safety cabinet or other primary containment device (see also Chapter 10) 4. Respiratory protective equipment may be necessary for some laboratory proceduresor working with animals infected with certain pathogens(see Chapter 11)
3. Open manipulations of all potentially infectious material must be conducted within a biological safety cabinet or other primary containment device (see also Chapter 10). 4. Respiratory protective equipment may be necessary for some laboratory proceduresor working with animals infected with certain pathogens (see Chapter 11)
Laboratory design and facilities
Laboratory design and facilities