Stewardship in Biotechnology Research 研究为生物技术使用服务 A few examples within the College of Agriculture l。 wa State University 依阿华州立大学农学院简例 Dr Mark Westgate 马克维斯盖特博士 Professor of Crop Production and Physiology Department of Agronomy 农学系作物生产和生理教授 IOWA STATE UNⅣ ERSITY College of Agriculture
Stewardship in Biotechnology Research 研究为生物技术使用服务 A few examples within the College of Agriculture Iowa State University 依阿华州立大学农学院简例 Dr. Mark Westgate 马克.维斯盖特博士 Professor of Crop Production and Physiology Department of Agronomy 农学系作物生产和生理教授
Research on Transgenic Corn 转基因玉米研究 ·改良玉米的质量 Improving maize quality traits 博士:美国农科 Dr Paul Scott, USDA-ARS and De partment of 学农学系教受 Agronomy 在玉米上生产动物疫苗 Animal vaccines in corne Plant sciences Institute Biopharmaceutical Initiative: 魏翔钙犯霍重￥额周 Dr Kan Wang, Director Plant Transformation Facility ·基因限界花粉散布的模拟 Gene Confinements 农学系教授马克维斯盖特博 Modeling Pollen Dispersal,DrMark Westgate, Department of Agronomy Economic cost of Uncontrolled pollen flowr 战禁糯奕动(漂移造 Dr Dermot Hayes, Pioneer Chair of Agribusines 商学院教授德莫特海斯博溥士 · BIGMA? Biosafety Institutefor Genetically Modified Agricultural Products,Dr Manjit Misra, Director Seed Science Center IOWA STATE UNⅣ ERSITY College of Agriculture
Research on Transgenic Corn 转基因玉米研究 • 改良玉米的质量 保尔 斯科特博士，美国农科 院和州立大学农学系教授 • 在玉米上生产动物疫苗 植物科学研究所生物药用研 究所植物转化主任王侃博士 • 基因限界,花粉散布的模拟 农学系教授马克. 维斯盖特博 士 • 非限制花粉流动（漂移〕造 成的经济损失 商学院教授德莫特 海斯博士 • 遗传修饰农业产品生物安全 研究所,种子科学中心主任满 基特密斯瑞博士 • Improving maize quality traits: Dr. Paul Scott, USDA-ARS and Department of Agronomy • Animal vaccines in corn: Plant Sciences Institute Biopharmaceutical Initiative: Dr. Kan Wang, Director Plant Transformation Facility • Gene Confinement: Modeling Pollen Dispersal, Dr. Mark Westgate, Department of Agronomy • Economic Cost of Uncontrolled Pollen Flow: Dr. Dermot Hayes, Pioneer Chair of Agribusiness • BIGMAP: Biosafety Institutefor Genetically Modified Agricultural Products, Dr. Manjit Misra, Director Seed Science Center
Maize quality traits des gricultural Research 玉米质量性状 Dr. Scotts lab is using·斯科特博士的实 a combination of biochemistry, 验室综和利用生 molecular biology and 物化学,分子生 traditional plant breeding to add value 物学和传统植物 to corn 育种方法为玉米 增加价值。 IOWA STATE UNⅣ ERSITY College of Agriculture
• Dr.Scott’s lab is using a combination of biochemistry, molecular biology and traditional plant breeding to add value to corn • 斯科特博士的实 验室综和利用生 物化学，分子生 物学和传统植物 育种方法为玉米 增加价值。 Maize quality traits 玉米质量性状
Improving swine nutrition 物圆改良猪肉营养 alpha lactalbumin Sows milk contains alpha lactalbumin 母猪的牛奶含a 乳清白蛋 Alpha lactalbumin rotein is an ideal corn a乳清白蛋 eed additive 理想的玉釆饲料添加 剂 Dr Scott eveloped transgenic corn to 斯科特博士的转基因 roduce this protein in the seed 玉米在种子中生产该 蛋白 Lines are being developed for feeding trials 鑫裔議駱系目前 IOWA STATE UNⅣ ERSITY College of Agriculture
• Sow’s milk contains alpha lactalbumin • Alpha lactalbumin protein is an ideal corn feed additive • Dr. Scott eveloped transgenic corn to produce this protein in the seed • Lines are being developed for feeding trials • 母 猪 的 牛 奶 含 ａ -乳 清 白 蛋 白 • ａ-乳 清 白 蛋 白是 理想的玉米饲料添加 剂 • 斯科特博士的转基因 玉米在种子中生产该 蛋白 • 含该基因的品系目前 在饲喂试验中 alpha lactalbumin Improving swine nutrition 改 良 猪 肉 营 养
Animal vaccines in Ci 玉米中的动物疫苗 E.co∥ Dr. Wang's group developed transgenic 王博士的研究小组研 Co orn plants that produce究转基因玉米产生有 a tullytunctional vaccine活性功能的抗大肠杆 toxin 菌毒素的疫苗抗体 ° Part of the Plant Science·该研究是州立大学植 pharmaceutical Initiative物研究所项目之 Feeding antigen 喂养老鼠含抗体的玉 rotected them againste.米使老鼠不受抗大肠 coli enterotoxin and cholera toxin 杆菌葡萄球菌毒素和 霍乱弧菌毒素危害 IOWA STATE UNⅣ ERSITY College of Agriculture
Animal vaccines in corn 玉米中的动物疫苗 • 王博士的研究小组研 究转基因玉米产生有 活性功能的抗大肠杆 菌毒素的疫苗抗体 • 该研究是州立大学植 物研究所项目之一 • 喂养老鼠含抗体的玉 米使老鼠不受抗大肠 杆菌葡萄球菌毒素和 霍乱弧菌毒素危害 • Dr. Wang’s group developed transgenic corn plants that produce a fully functional vaccine antigen against an E.coli toxin. • Part of the Plant Science Institute Biopharmaceutical Initiative • Feeding antigencontaining corn to mice protected them against E. coli enterotoxin and cholera toxin E. coli
Stewardship of plant-Made Pharmaceuticals and Industrials 为植物产药用工业服务 Scientific issues 科学领城:证明概念保 Proof of concept, Genetic stability 证遗传稳定性 Regulatory requirements: 管理要求:产品的可比性, Product comparability, 纯度 pun Safety concerns 安全性考虑环境效应, Environmental impact, 产品保护和基因漂移 Product protection, Gene flow ·商品化:经济和法律问题 Commercialization Economic and Legal issues IOWA STATE UNⅣ ERSITY College of Agriculture
• Scientific issues: Proof of concept, Genetic stability • Regulatory requirements: Product comparability, purity • Safety concerns: Environmental impact, Product protection, Gene flow • Commercialization: Economic and Legal issues • 科学领域: 证明概念,保 证遗传稳定性 • 管理要求:产品的可比性, 纯度 • 安全性考虑:环境效应, 产品保护和基因漂移 • 商品化:经济和法律问题 Stewardship of Plant-Made Pharmaceuticals and Industrials 为植物产药用工业服务
Confinement requirements in open field trials 出间试验的隔离要求 Physical isolation 1 物理隔离与其他玉 mile(1600m)from 米相隔大于1英里 any other corn (1600米) Temporal isolation planted t 28 days 时间隔离在1600米 from any other 内与其他玉米播种 corn within1600m期相隔28天提早或 退后) APHIS- Biotechnology Regulatory Services visits each 农部动植物检疫中心对 site to check 每个试验点进行田间 检查 IOWA STATE UNⅣ ERSITY College of Agriculture
Confinement requirements in open field trials 田间试验的隔离要求 • 物理隔离:与其他玉 米相隔大于1英里 (1600米) • 时间隔离:在1600米 内,与其他玉米播种 期相隔28天(提早或 退后) 农部动植物检疫中心对 每个试验点进行田间 检查 APHIS – Biotechnology Regulatory Services visits each site to check Physical isolation: 1 mile (1600m) from any other corn Temporal isolation: planted ± 28 days from any other corn within 1600 m
2004 Colorado ffield site managed by Horan Brothers, Agricultural Enterprizes 2004年科罗拉多田间试验点是郝润兄弟农业企业 Physical isolation 物理隔离:与最近的 More than 4800 m from nearest corn 玉米田相隔大于 field 4800米 I Temporal isolation Planted 28 days 时间隔离在周围玉 after nearest corn 米种植后28天播种 field APS- JotecinoJo:/农部动植物检疫中心将 there to check N 对试验点进行田间检 「查 IOWA STATE UNⅣ ERSITY College of Agriculture
2004 Colorado field site managed by Horan Brothers, Agricultural Enterprizes 2004 年科罗拉多田间试验点是郝润兄弟农业企业 • Physical isolation: – More than 4800 m from nearest corn field • Temporal isolation: – Planted 28 days after nearest corn field • 物理隔离:与最近的 玉米田相隔大于 4800米 • 时间隔离:在周围玉 米种植后28天播种 农部动植物检疫中心将 对试验点进行田间检 查 APHIS – Biotechnology Regulatory Services was there to check
Managing Transgene Flow: 转基因流动的管理 Pollen production Flower development ·花粉的产生 and function 漂移因子的产生和功能 Physical dispersal in air 在空气中的物理飘散 Atmospheric conditions: wind 大气条件风速和风向, speed and direction, stability index, 稳定指数温度和相对湿 temperature, relative 度 humidity ·授粉的生物学 Pollination biology Synchrony with female 与雌性花粉漂移因子发 flower development 生和功能的同步性 and function IOWA STATE UNⅣ ERSITY College of Agriculture
Managing Transgene Flow: 转基因流动的管理 • Pollen production – Flower development and function • Physical dispersal in air – Atmospheric conditions: wind speed and direction, stability index, temperature, relative humidity • Pollination biology – Synchrony with female flower development and function • 花粉的产生 - 漂移因子的产生和功能 • 在空气中的物理飘散 - 大气条件:风速和风向, 稳定指数,温度和相对湿 度 • 授粉的生物学 - 与雌性花粉漂移因子发 生和功能的同步性
Juy21,2000 Modeling pollen dispersal 花粉飘散的模拟 1 polen grain am Dr. Westgate and 维斯盖特博薄士和同 colleagues have 事建立了计算机模 developed computer型计算花粉漂移数 models to calculate the amount of 量和转基因玉米日 pollen drift and the 间花粉基因产生漂 potential for gene 移的可能性 flow from a field of transgenic corn IOWA STATE UNⅣ ERSITY College of Agriculture
• Dr. Westgate and colleagues have developed computer models to calculate the amount of pollen drift and the potential for gene flow from a field of transgenic corn • 维斯盖特博士和同 事建立了计算机模 型计算花粉漂移数 量和转基因玉米田 间花粉基因产生漂 移的可能性 Modeling pollen dispersal 花粉飘散的模拟 July 21, 2000 g/m2 1 pollen grain/4 cm2 at 0.25 pollen grain/cm2d potential contamination ~ 0.28% or ~ 3 kernels per 1000 exposed silks 100m July 21, 2000 g/m2 1 pollen grain/4 cm2 at 0.25 pollen grain/cm2d potential contamination ~ 0.28% or ~ 3 kernels per 1000 exposed silks 100m