Leishmania donovani
Leishmania donovani ___________________
Charles Louis Alphonse Laveran France Institute Pasteur Paris, france 1845-1922 The nobel prize in Physiology or Medicine 1907 in recognition of his work on the role played by protozoa in causing diseases
Charles Louis Alphonse Laveran France Institute Pasteur Paris, France 1845 - 1922 The Nobel Prize in Physiology or Medicine 1907 "in recognition of his work on the role played by protozoa in causing diseases
Geographic distribution of Visceral leishmaniasis F Leishmaniasis is prevalent world wide: ranging from south east Asia, Indo-Pakistan, Mediterranean north and central Africa, and south and central america
Leishmaniasis is prevalent world wide: ranging from south east Asia, Indo-Pakistan, Mediterranean, north and central Africa, and south and central America
Species Pathogenic in Humans Leishmania donovani( Laveran 1903) Leishmania tropica(CL) Leishmania major(CL) Leishmania aethiopica(CL) LEishmania mexicana(CL) Leishmania braziliensis cl)
Species Pathogenic in Humans Leishmania donovani (Laveran 1903) (VL) Leishmania tropica (CL) Leishmania major (CL) Leishmania aethiopica (CL) Leishmania mexicana (CL) Leishmania brazilliensis (MCL)
Morphology Digenetic Life Cycle Promastigote Amastigote > Insect Mammalian stage Motile Non-motile Midgut > Intracellular
Morphology ▪ Promastigote ➢ Insect ➢ Motile ➢ Midgut ▪ Amastigote ➢ Mammalian stage ➢ Non-motile ➢ Intracellular Digenetic Life Cycle
Morphology Amastigote(leishman-donovan body) Human phase reside in macrophage >Very, very minute elliptical body > No free flagellum >Nucleus: deep red, located at one side >Cytoplasm: blue(after right stain) Kinetoplast; basal body rhizoplast
Morphology ▪ Amastigote (leishman-donovan body): ➢Human phase, reside in macrophage ➢Very, very minute elliptical body ➢No free flagellum ➢Nucleus: deep red, located at one side ➢Cytoplasm: blue (after right stain) ➢Kinetoplast; basal body; rhizoplast
Promastigote elongate form anterior PROMASTIGOTE 15-30 um long Flagellum one nucleus, red stained, Basal bod +Kinetoplast central in position one anterior flagellum which is just as long Nucleus as its body length. a rod like kinetoplast posterior Insect a dot-like basal body Motile Midgut
Promastigote elongate form - 15 - 30 µm long one nucleus, red stained, central in position, • one anterior flagellum which is just as long as its body length. • a rod-like kinetoplast • a dot-like basal body Flagellum Basal body Kinetoplast Nucleus anterior posterior Insect Motile Midgut
a Promastigote >Vector phase Reside in the gut of sandfly Spindle shaped with 1 free flagellum Nucleus; cytoplasm; kinetoplast; basal body; rhizoplast Chrysanthemum-like in culture medium
▪ Promastigote: ➢Vector phase ➢Reside in the gut of sandfly ➢Spindle shaped with 1 free flagellum ➢Nucleus; cytoplasm; kinetoplast; basal body; rhizoplast ➢Chrysanthemum-like in culture medium
Leishmania A macrophage filled with Leishmania amastigotes (by Mike Belosevic
▪ A macrophage filled with Leishmania amastigotes
Amastigote of L donovani (under oil immersion objective) 0.C 8x8
Amastigote of L. donovani (under oil immersion objective)
