Oncogene dominant gain-of-function mutations promote cell transformation Tumor suppressor gene recessive,loss-of-function mutations promote cell transformation
Oncogene — dominant gain-of-function mutations promote cell transformation Tumor suppressor gene — recessive, loss-of-function mutations promote cell transformation
Images removed due to copyright reasons
Images removed due to copyright reasons
Daughter cells Extracellular growth control signals Go Intracellular quality control checks M(Mitosis) G1 G2 S (DNA synthesis) Figure by MIT OCW
growth checks (DNA synthesis) S G1 G0 G2 Extracellular control signals Intracellular quality control Daughter cells M (Mitosis) Figure by MIT OCW
Cell cycle in S.cerevisiae Mother cell Cytokinesis Daughter cell Chromosome segregation; Growth nuclear division M G1 START Spindle pole body duplication Spindle formation Bud emergence DNA replication Nuclear migration Figure by MIT OCW
Cell cycle in S. cerevisiae Mother cell Growth Spindle pole body duplication Spindle formation Chromosome segregation; Cytokinesis M G1 G2 S Daughter cell START Bud emergence DNA replication Nuclear migration nuclear division Figure by MIT OCW
Yeast in liquid culture Incubate at 23 C for 5 h Add mutagen; distribute into 0000000 smaller aliquots Plate out individual Colonies aliquots Agar Incubate at 23C Temperature-sensitive Replica-plate and for growth;growth at23°， incubate no growth at 36 23C 36℃ A Genetic Screen for Cell Cycle Mutants Figure by MIT OCW
A Genetic Screen for Cell Cycle Mutants Figure by MIT OCW