Medical Genetics Gene Mapping cloning
Gene Mapping & Cloning Medical Genetics
Gene Mapping Determination of the relative positions of genes on a DNA molecule(chromosome and of the distance, in linkage units or hysical units, between them o 1911, red-green color blindness localized to chromosome x 1968, Duffy Blood Group localized to chromosome 1
Determination of the relative positions of genes on a DNA molecule (chromosome) and of the distance, in linkage units or physical units, between them. Gene Mapping ❖ 1911,red-green color blindness localized to chromosome X ❖ 1968, Duffy Blood Group localized to chromosome 1
Linkage Analysis A mathematical procedure that analyzes meiotic recombination frequencies between pairs of genes to determine whether two loci are linked and, if so how closely
Linkage Analysis A mathematical procedure that analyzes meiotic recombination frequencies between pairs of genes to determine whether two loci are linked and, if so, how closely
Linkage Analysis A mathematical procedure that analyzes meiotic recombination frequencies between pairs of genes to determine whether two loci are linked and, if so how closely
Linkage Analysis A mathematical procedure that analyzes meiotic recombination frequencies between pairs of genes to determine whether two loci are linked and, if so, how closely
Linkage Analysis A mathematical procedure that analyzes meiotic recombination frequencies between pairs of genes to determine whether two loci are linked and, if so how closely
Linkage Analysis A mathematical procedure that analyzes meiotic recombination frequencies between pairs of genes to determine whether two loci are linked and, if so, how closely
Linkage Analysis A mathematical procedure that analyzes meiotic recombination frequencies between pairs of genes to determine whether two loci are linked and, if so how closely Recombination fraction Centimorgan, cM
Linkage Analysis A mathematical procedure that analyzes meiotic recombination frequencies between pairs of genes to determine whether two loci are linked and, if so, how closely. ❖ Recombination fraction ❖ Centimorgan, cM
Genetic Marker a genetic marker is a tag which allows to identify a place in a genome(locus), is atag near or within a gene of interest. genetic markers are usually highly polymorphic
Genetic Marker A genetic marker is a tag which allows to identify a place in a genome (locus), is a ‘tag’ near or within a gene of interest. Genetic markers are usually highly polymorphic
Genetic Marker g Blood group; Serum protein; HLA ☆ DNA RFLP dNA STR DNA VNtR Single nucleotide polymorphism, SNP
Genetic Marker ❖ Blood group; Serum protein; HLA ❖ DNA RFLP ❖ DNA STR & DNA VNTR ❖ Single nucleotide polymorphism, SNP
Linkage Analysis Neurofibroma, NFl RFLP Ⅲ 2345678 ECoR I ECOR I ECoR I 3.0kb 1.7 kb Probe
Linkage Analysis EcoRⅠ EcoRⅠ EcoRⅠ 3.0 kb 1.7 kb Probe Neurofibroma, NF1 RFLP Ⅰ Ⅱ Ⅲ 1 2 1 2 3 4 5 6 7 8 1 2 3 4 5 6 7 8
Linkage Analysis Neurofibroma, NFl RFLP Ⅲ 2345678 3.0kb 1kb 4.7kb
Linkage Analysis Neurofibroma, NF1 RFLP Ⅰ Ⅱ Ⅲ 1 2 1 2 3 4 5 6 7 8 1 2 3 4 5 6 7 8 3.0 kb 1.7 kb 4.7 kb
