Cell Biosciences CELL BIOSCIENCES,INC. FluorChem HD2 and FC2 User Guide Copyright2010 Cell Biosciences,Inc.All rights reserved. 9692 FlorChem HD2 and FC2 User Guide
page 1 FluorChem HD2 and FC2 User Guide CELL BIOSCIENCES, INC. FluorChem HD2 and FC2 User Guide Copyright © 2010 Cell Biosciences, Inc. All rights reserved. Cell Biosciences, Inc. 3040 Oakmead Village Drive Santa Clara, CA 95051 Toll-free: (888) 607-9692 Tel: (408) 510-5500 Fax: (408) 510-5599 email: support@cellbiosciences.com web: www.cellbiosciences.com
FluorChem HD2 and FC2 User Guide P/N94-15068-00 Revision 2,June 2010 For research use only.Not for use in diagnostic procedures Patents and Trademarks Automatic Image Capture (AIC)and digital ProteomeChip technology are covered by U.S.Patent Nos 6.995,901,6.909,459,6.853,454.6.271,042,7,166.202,and other issued and pending patents in the U.S.and tein Forest logo,the AIC logo,AlphaCal Alphalmager.AlphaPartIIView,AlphaQuant AlphaSnap.AlphaSpec. AlphaUV.AlphaView,ChemiGlow,Chromalight dPC.digital ProteomeChip,FluorChem,MSRAT.Multilmage. NanoPro,the intertwined helix design,iWB,ProteomeChip.red,SpectraPlex,Xpedition,XplorBright,and XplorUV are trademarks or registered trademarks of Cell Biosciences,Inc.Other marks appearing in these materials are marks of their respective owners FluorChem HD2 and FC2User Guid
FluorChem HD2 and FC2 User Guide FluorChem HD2 and FC2 User Guide P/N 94-15068-00 Revision 2, June 2010 For research use only. Not for use in diagnostic procedures Patents and Trademarks Automatic Image Capture (AIC) and digital ProteomeChip technology are covered by U.S. Patent Nos 6,995,901, 6,909,459, 6,853,454, 6,271,042, 7,166,202, and other issued and pending patents in the U.S. and other countries. Cell Biosciences, the Cell Biosciences logo, the Alpha Innotech logo, Protein Forest, the Protein Forest logo, the AIC logo, AlphaCal, AlphaImager, AlphaPart11View, AlphaQuant, AlphaSnap, AlphaSpec, AlphaUV, AlphaView, ChemiGlow, Chromalight, dPC, digital ProteomeChip, FluorChem, MSRAT, MultiImage, NanoPro, the intertwined helix design, iWB, ProteomeChip, red, SpectraPlex, Xpedition, XplorBright, and XplorUV are trademarks or registered trademarks of Cell Biosciences, Inc. Other marks appearing in these materials are marks of their respective owners
Table of Contents Chapter l: Contrast Adjustments.........................44 Introduction and Setup....................I Using the Contrast Adjustments Tools for Grayscale Introduction to the FluorChem Systems and AlphaView m0ges.............45 Software.... …2 Automatic Enhancement...................51 Mouse Functions......... Imaging Tools....... 5 Starting AlphaView Software................. 2 Tool Bar..........5 About This Manual....... 3 Compare View....53 Customer Service and Technical Support...........3 Tool Box..56 FluorChem System Setup.......... Status Bar...... System Components....... .56 .4 System Placement........ .4 Chapter3: Cable Connections..................4 Drop-Down Menus.....................57 Hardware Installation.....5 Main Menu.… .58 Power Up Sequence........ …14 File Menu.. .58 System Information........................5 File Open Menu..................... .58 File Save/Load Analysis.................. File>Close Menu. 60 File Save,Save As,Save Modified,and Save All Basic lmaging Functions...8 Menus..................................60 Acquiring an Image Using the Navigator........8 Acquiring an Image Using the Acquire Button...24 Printer Setup........ .62 Automatic Image Capture (AIC Software-Optional.38 Exit Function............................63 Designing a User Protocol..................39 Edit Menu............ .64 Auto Image Capture (AIC)..................40 Edit Activation...............64 AlphaView System Quick Guide. .42 Copy and Crop...... 65 FlorChem HD2 and FC2 User Guide
page i FluorChem HD2 and FC2 User Guide Chapter 1: Introduction and Setup. . . . . . . . . . . . . . . . . . . . 1 Introduction to the FluorChem Systems and AlphaView Software. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Mouse Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Starting AlphaView Software. . . . . . . . . . . . . . . . . . 2 About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Customer Service and Technical Support. . . . . . . . . . . . 3 FluorChem System Setup . . . . . . . . . . . . . . . . . . . . . . . . 4 System Components . . . . . . . . . . . . . . . . . . . . . . . . 4 System Placement . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Cable Connections. . . . . . . . . . . . . . . . . . . . . . . . . . 4 Hardware Installation . . . . . . . . . . . . . . . . . . . . . . . 5 Power Up Sequence . . . . . . . . . . . . . . . . . . . . . . .14 System Information . . . . . . . . . . . . . . . . . . . . . . . .15 Chapter 2: Getting Started—Basic Imaging Functions . 17 Basic Imaging Functions . . . . . . . . . . . . . . . . . . . . . . . .18 Acquiring an Image Using the Navigator. . . . . . .18 Acquiring an Image Using the Acquire Button. . .24 Automatic Image Capture (AIC) Software–Optional .38 Designing a User Protocol. . . . . . . . . . . . . . . . . . .39 Auto Image Capture (AIC) . . . . . . . . . . . . . . . . . .40 AlphaView System Quick Guide . . . . . . . . . . . . . . . . . .42 Contrast Adjustments . . . . . . . . . . . . . . . . . . . . . . . . . . 44 Using the Contrast Adjustments Tools for Grayscale Images . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45 Automatic Enhancement . . . . . . . . . . . . . . . . . . . 51 Imaging Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 Tool Bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51 Compare View. . . . . . . . . . . . . . . . . . . . . . . . . . . . 53 Tool Box. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 Status Bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56 Chapter 3: Drop-Down Menus . . . . . . . . . . . . . . . . . . . . . . . 57 Main Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 File Menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58 File > Open Menu . . . . . . . . . . . . . . . . . . . . . . . . 58 File > Save/Load Analysis. . . . . . . . . . . . . . . . . . . 59 File > Close Menu. . . . . . . . . . . . . . . . . . . . . . . . . 60 File Save, Save As, Save Modified, and Save All Menus . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60 Print . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 Printer Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62 Exit Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 Edit Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 Edit Activation . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64 Copy and Crop . . . . . . . . . . . . . . . . . . . . . . . . . . . 65 Table of Contents
page ii Reset and Clear Options...................65 Chapter 4: m0 ge Menu..........65 Image Enhancement Tools...............85 0vey....66 Tool Box Enhancement Tab.....................8 Equqlize.................................66 Z00m. .86 Arithmetic................. .....66 Hist0gam..............87 Conversion............ ..67 Rotate-Fip. 89 Flat Field Calibrate (Manua). 68 Annotgte.............................. 90 Resize...................................69 Object Attributes... .91 Image Information......... ...69 Drawing Tools....... .95 Setup Menu..............................0 Editing Too水..97 Prnt info................................. False Color............ ....99 Print Mode..................... Gray Scale (Palette 0)... ….100 Print Date......... 72 High-Low (Palette I)..................00 Preferences...........................2 Other Palettes..... System Calibration.........................6 Filter............. .102 0 verlay Menu.............76 General Information. .103 Sharpening Filters. .104 Save Overlay.....7 Noise Filters....... 104 Despeckle Filters.. 104 Show Annotation..........................78 3-D (Contour)Filters........5 Utilities Menu...... .78 Smoothing Filters...............06 Notepad. Edge Filters. ..106 Explorer....... ..79 Horizontal Edge Filter. .06 View Menu.. .80 Vertical Edge Filter 106 Custom Filter. Default Tools Position.... .107 ……82 Undo Button..... ..107 Zoom Functions.. Window Menu. .82 Examples of Filter Results 107 Help Menu.… .83 Movie Mode.......... .107 FlorChem HD2 and FC2 User Guide
page ii FluorChem HD2 and FC2 User Guide Reset and Clear Options. . . . . . . . . . . . . . . . . . . .65 Image Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .65 Overlay . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .66 Equalize . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .66 Arithmetic. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .66 Conversion . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .67 Flat Field Calibrate (Manual) . . . . . . . . . . . . . . . .68 Resize. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .69 Image Information . . . . . . . . . . . . . . . . . . . . . . . . .69 Setup Menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .70 Print Info. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71 Print Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71 Print Date. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .72 Preferences. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .72 System Calibration. . . . . . . . . . . . . . . . . . . . . . . . .76 Overlay Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .76 Load Overlay . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77 Save Overlay . . . . . . . . . . . . . . . . . . . . . . . . . . . . .77 Overlay Libraries . . . . . . . . . . . . . . . . . . . . . . . . . .78 Show Annotation . . . . . . . . . . . . . . . . . . . . . . . . . .78 Utilities Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .78 Notepad. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79 Explorer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .79 View Menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .80 Default Tools Position . . . . . . . . . . . . . . . . . . . . . .81 Zoom Functions. . . . . . . . . . . . . . . . . . . . . . . . . . .82 Window Menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .82 Help Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .83 Chapter 4: Image Enhancement Tools . . . . . . . . . . . . . . . 85 Tool Box Enhancement Tab. . . . . . . . . . . . . . . . . . . . . 86 Zoom. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86 Histogram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87 Rotate-Flip. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 Annotate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90 Object Attributes . . . . . . . . . . . . . . . . . . . . . . . . . . 91 Drawing Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95 Editing Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97 False Color. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 99 Gray Scale (Palette 0). . . . . . . . . . . . . . . . . . . . .100 High–Low (Palette 1) . . . . . . . . . . . . . . . . . . . . .100 Other Palettes . . . . . . . . . . . . . . . . . . . . . . . . . . .100 Filter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .102 General Information . . . . . . . . . . . . . . . . . . . . . .103 Sharpening Filters . . . . . . . . . . . . . . . . . . . . . . . .104 Noise Filters. . . . . . . . . . . . . . . . . . . . . . . . . . . . .104 Despeckle Filters . . . . . . . . . . . . . . . . . . . . . . . . .104 3-D (Contour) Filters. . . . . . . . . . . . . . . . . . . . . .105 Smoothing Filters . . . . . . . . . . . . . . . . . . . . . . . . .106 Edge Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . .106 Horizontal Edge Filter. . . . . . . . . . . . . . . . . . . . .106 Vertical Edge Filter . . . . . . . . . . . . . . . . . . . . . . .106 Custom Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . .107 Undo Button . . . . . . . . . . . . . . . . . . . . . . . . . . . .107 Examples of Filter Results . . . . . . . . . . . . . . . . .107 Movie Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .107
Chapter 5: Mass Standards..... .165 Image Analysis Tools.. …109 Advanced Lane Profile Options............70 Tool Box Analysis Tools Tab..... …110 Common Features. .180 Molecular Weight Detemmination. .110 Additional Analysis Tools .184 Entering Known Molecular Weights for Markers… 112 The Ruler Function .85 Determining Molecular Weights of Unknown The Scoring Function 186 Band5...... 113 Manual Count...... 88 Using the Molecular Weight Standards Library 115 Analyzing Amays..... …190 Special Functions....17 Lane Profile-Advanced. .194 The Groph Tool.........................18 Common Export Result Feature .194 Colony Count....................119 Editing Toos..............123 Appendix A: Opening AlphaView Files in Other Software Spot Count Data......125 Programs...............................197 Band Analysis Tools............ 126 Viewing AlphaView Images......................98 Creating an Object Area of Interest.........126 Using ResEdit..............................98 Magic Wand and AutoSpot..8 Manipulating Objects...... 132 Appendix B: AlphaView Molecular Weight Library Files 201 Band Analysis Measurements .133 List of Standards..02 Background Tab-Calculating Background Values....... ..135 Mass Standard Calibration Curves for Quantitative Appendix C: Data Description Tables..................205 PCR.. 37 Single Channel Columns.....................206 Lane Profile 140 Profle Path Width.. Appendix D: Auto Lane Detection Using an Area of Interest14 Bias and Dark Master Utility..............09 Manual Lane Detection.............42 Bias and Darkmaster File Generation............210 Bands Darker than Background Option......144 Appendix E: Image Analysis-Detect Bands …144 Flat Field Calibration..............3 Viewing Results..........................44 Gel Smiling Correction.....................57 Calibration Procedure. .214 Molecular Weight Tab..............6 FlorChem HD2 and FC2 User Guide
page iii FluorChem HD2 and FC2 User Guide Chapter 5: Image Analysis Tools. . . . . . . . . . . . . . . . . . . . 109 Tool Box Analysis Tools Tab . . . . . . . . . . . . . . . . . . . 110 Molecular Weight Determination. . . . . . . . . . . . . . . 110 Entering Known Molecular Weights for Markers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112 Determining Molecular Weights of Unknown Bands . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 Using the Molecular Weight Standards Library 115 Special Functions . . . . . . . . . . . . . . . . . . . . . . . . 117 The Graph Tool . . . . . . . . . . . . . . . . . . . . . . . . . 118 Colony Count . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119 Editing Tools. . . . . . . . . . . . . . . . . . . . . . . . . . . . 123 Spot Count Data . . . . . . . . . . . . . . . . . . . . . . . . 125 Band Analysis Tools. . . . . . . . . . . . . . . . . . . . . . . . . . 126 Creating an Object Area of Interest . . . . . . . . . 126 Magic Wand and AutoSpot . . . . . . . . . . . . . . . 128 Manipulating Objects . . . . . . . . . . . . . . . . . . . . 132 Band Analysis Measurements . . . . . . . . . . . . . 133 Background Tab–Calculating Background Values. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135 Mass Standard Calibration Curves for Quantitative PCR. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137 Lane Profile . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 140 Profile Path Width . . . . . . . . . . . . . . . . . . . . . . . 141 Auto Lane Detection Using an Area of Interest141 Manual Lane Detection . . . . . . . . . . . . . . . . . . 142 Bands Darker than Background Option. . . . . . 144 Image Analysis–Detect Bands . . . . . . . . . . . . . 144 Viewing Results . . . . . . . . . . . . . . . . . . . . . . . . . 144 Gel Smiling Correction . . . . . . . . . . . . . . . . . . . . 157 Molecular Weight Tab . . . . . . . . . . . . . . . . . . . 161 Mass Standards. . . . . . . . . . . . . . . . . . . . . . . . . .165 Advanced Lane Profile Options . . . . . . . . . . . . .170 Common Features. . . . . . . . . . . . . . . . . . . . . . . .180 Additional Analysis Tools . . . . . . . . . . . . . . . . . . . . . .184 The Ruler Function . . . . . . . . . . . . . . . . . . . . . . .185 The Scoring Function . . . . . . . . . . . . . . . . . . . . . .186 Manual Count . . . . . . . . . . . . . . . . . . . . . . . . . . .188 Analyzing Arrays . . . . . . . . . . . . . . . . . . . . . . . . .190 Lane Profile - Advanced . . . . . . . . . . . . . . . . . . .194 Common Export Result Feature . . . . . . . . . . . .194 Appendix A: Opening AlphaView Files in Other Software Programs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .197 Viewing AlphaView Images. . . . . . . . . . . . . . . . . . . . .198 Using ResEdit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .198 Appendix B: AlphaView Molecular Weight Library Files 201 List of Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . .202 Appendix C: Data Description Tables . . . . . . . . . . . . . . . . . .205 Single Channel Columns. . . . . . . . . . . . . . . . . . . . . . .206 Appendix D: Bias and Dark Master Utility . . . . . . . . . . . . . .209 Bias and Darkmaster File Generation. . . . . . . . . . . .210 Appendix E: Flat Field Calibration . . . . . . . . . . . . . . . . . . . . .213 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .214 Calibration Procedure . . . . . . . . . . . . . . . . . . . . . . . . .214
Chapter I: Introduction and Setup Chapter Overview Introduction to the FluorChem Systems and AlphaView Software ·About This Manual Customer Service and Technical Support FluorChem System Setup FlorChem HD2 and FC2 User Guide
page 1 FluorChem HD2 and FC2 User Guide Chapter 1: Introduction and Setup Chapter Overview • Introduction to the FluorChem Systems and AlphaView Software • About This Manual • Customer Service and Technical Support • FluorChem System Setup
page 2 Chapter:and Setup Introduction to the FluorChem Systems and AlphaView Software The FluorChem Systems are designed to meet the requirements of the most demanding chemiluminescence grate the latest developments in camera and lens technology for imaging chemiluminescent and fluorescent samples with a wide dynamic range and fast image acquisition speeds. The system is controlled by easy-to-use and intuitive AlphaView software which performs image analysis and archiving and can also prepare images for desktop publishing. AlphaView software includes image optimization tools that adjust contrast automatically or manually Convert- or other r such tech- niques can be used to clarify hard-to-see portions of the image.Notes,labels,amrows,lines,and other drawing tools can be recorded directly onto the image using AlphaView software's annotation functions.Annotations are superimposed on the image upon hard copy printing and can either be saved as a template file or as part of the image.All AlphaView features are accessible via convenient on-screen buttons and menus in an intui- tive.mouse-controlled interface. determination,lane profile,band analysis,quantitative PCR,microtiter plate reading,object distance measuring,gel scoring,and automatic colony counting. FluorChem systems include all the necessary hardware and software for image capture,enhancement,and analysis.AlphaView software does not require a dedicated computer system and can operate simultaneously with other office-related software,such as word processing programs,spreadsheets,and desktop publishing software Images can be printed using a 256-level gray scale thermal printer or any printer with a Windowsdriver.The low-cost,high-quality prints are ideal for lab notebook records or publication. The"AlphaView System Quick Guide"on page 42 provides a condensed instruction sheet that acts as a quick reference guide for users already familiar with the software.Keeping copies of the appropriate instruction sheet(s)near the system for easy reference is highly recommended. Mouse Functions The system comes packaged with a two-button mouse.The left button activates functions and makes selec- was most recently assigned to the left mouse button. Starting AlphaView Software osarptiVewgotaedobedktheAphaVewotaeionontewnioidehop FluorChem HD2 and FC2User Guid
page 2 Chapter 1: Introduction and Setup FluorChem HD2 and FC2 User Guide Introduction to the FluorChem Systems and AlphaView Software The FluorChem Systems are designed to meet the requirements of the most demanding chemiluminescence assays and also provide the most versatile fluorescence imaging system available today. These systems integrate the latest developments in camera and lens technology for imaging chemiluminescent and fluorescent samples with a wide dynamic range and fast image acquisition speeds. The system is controlled by easy-to-use and intuitive AlphaView® software which performs image analysis and archiving and can also prepare images for desktop publishing. AlphaView software includes image optimization tools that adjust contrast automatically or manually. Converting the image from positive to negative, using digital filters, applying a false color map, or other such techniques can be used to clarify hard-to-see portions of the image. Notes, labels, arrows, lines, and other drawing tools can be recorded directly onto the image using AlphaView software’s annotation functions. Annotations are superimposed on the image upon hard copy printing and can either be saved as a template file or as part of the image. All AlphaView features are accessible via convenient on-screen buttons and menus in an intuitive, mouse-controlled interface. AlphaView software also includes a broad array of analysis tools, including molecular weight calculation, Rf determination, lane profile, band analysis, quantitative PCR, microtiter plate reading, object distance measuring, gel scoring, and automatic colony counting. FluorChem systems include all the necessary hardware and software for image capture, enhancement, and analysis. AlphaView software does not require a dedicated computer system and can operate simultaneously with other office-related software, such as word processing programs, spreadsheets, and desktop publishing software. Images can be printed using a 256-level gray scale thermal printer or any printer with a Windows® driver. The low-cost, high-quality prints are ideal for lab notebook records or publication. The “AlphaView System Quick Guide” on page 42 provides a condensed instruction sheet that acts as a quick reference guide for users already familiar with the software. Keeping copies of the appropriate instruction sheet(s) near the system for easy reference is highly recommended. Mouse Functions The system comes packaged with a two-button mouse. The left button activates functions and makes selections when using the software. In some cases, the right mouse button can recall or reactivate the function that was most recently assigned to the left mouse button. Starting AlphaView Software To start AlphaView software, double-click the AlphaView software icon on the Windows desktop
About This Manual About This Manual This manual uses different fonts to indicate certain conditions: Bold text indicates the name of a button,a menu,or a function found in a menu. Courier text indicates an entry that is typed. Letters or words found between<>refer to keys on the keyboard .NOTE indicates key points and useful hints. CAUTION and !WARNING!statements indicate actions that may either harm the system or user. or affect the data quality. Icons or buttons to be pushed are placed next to their respective descriptions in the text. Customer Service and Technical Support The Cell Biosciences staff is available to respond to any questions or comments about the software.For ques- tions,new software feature ideas,or general feedback,use the following contact methods: Telephone (408)510-5500 (888)607-9692(toll firee) Fax (408)510-5599 E-mail support@cellbiosciences.com Web www.cellbiosciences.com Address Cell Biosciences.Inc Santa Clara.CA95051 USA FluorChem HD2 and FC2 User Guide
About This Manual page 3 FluorChem HD2 and FC2 User Guide About This Manual This manual uses different fonts to indicate certain conditions: • Bold text indicates the name of a button, a menu, or a function found in a menu. • Courier text indicates an entry that is typed. • Letters or words found between refer to keys on the keyboard. • NOTE indicates key points and useful hints. • CAUTION and !WARNING! statements indicate actions that may either harm the system or user, or affect the data quality. • Icons or buttons to be pushed are placed next to their respective descriptions in the text. Customer Service and Technical Support The Cell Biosciences staff is available to respond to any questions or comments about the software. For questions, new software feature ideas, or general feedback, use the following contact methods: Telephone (408) 510-5500 (888) 607-9692 (toll free) Fax (408) 510-5599 E-mail support@cellbiosciences.com Web www.cellbiosciences.com Address Cell Biosciences, Inc. 3040 Oakmead Village Drive Santa Clara, CA 95051 USA
Chapter:and Setup FluorChem System Setup System Components The FluorChem systems include the following .High-performance,high resolution,CCD camera Manual f/2.8 28-70mm zoom lens or other optional lens Computer with keyboard,mouse,and monitor (optional) Windows operating system (pre-installed) AlphaView image processing and analysis software(pre-installed and calibrated with computer and hardware system) Multilmage l FC light cabinet containing a UV transilluminator.white light fold-down transilluminator and epi white lights with interference filter ·Fast lens(optional) Epi-illuminating UV lights(optional 254nm or 365nm) ·Printer(optional ChromaLight(optional) Upon receiving the system,it is critical to check the enclosed packing list to verify that all components are properly included. System Placement As with all electrical instruments,the FluorChem systems should be located on a table or bench top that is dry and stable,and away from water,solvents,or corrosive materials.In addition,the system should be placed away from interfering electrical signals and magnetic fields.If possible,a dedicated electrical outlet should be used to eliminate electrical interference from other laboratory instrumentation. Cable Connections The cable connectors and their respective mating ports are keyed or unique for each connection to eliminate potential wrong mating.The connections are illustrated and described later in this chapter WARNING Make sure that the power is OFF and all power cords are disconnected while connecting the cables and setting up the system. FluorChem HD2 and FC2 User Guide
page 4 Chapter 1: Introduction and Setup FluorChem HD2 and FC2 User Guide FluorChem System Setup System Components The FluorChem systems include the following: • High-performance, high resolution, CCD camera • Manual f/2.8 28-70mm zoom lens or other optional lens • Computer with keyboard, mouse, and monitor (optional) • Windows operating system (pre-installed) • AlphaView image processing and analysis software (pre-installed and calibrated with computer and hardware system) • MultiImage® II FC light cabinet containing a UV transilluminator, white light fold-down transilluminator and epi white lights with interference filter • Fast lens (optional) • Epi-illuminating UV lights (optional 254nm or 365nm) • Printer (optional) • ChromaLight (optional) Upon receiving the system, it is critical to check the enclosed packing list to verify that all components are properly included. System Placement As with all electrical instruments, the FluorChem systems should be located on a table or bench top that is dry and stable, and away from water, solvents, or corrosive materials. In addition, the system should be placed away from interfering electrical signals and magnetic fields. If possible, a dedicated electrical outlet should be used to eliminate electrical interference from other laboratory instrumentation. Cable Connections The cable connectors and their respective mating ports are keyed or unique for each connection to eliminate potential wrong mating. The connections are illustrated and described later in this chapter. !WARNING! Make sure that the power is OFF and all power cords are disconnected while connecting the cables and setting up the system
FluorChem System Setup Hardware Installation All software.peripheral drivers,and operating systems come factory-installed on computers purchased directly from Cell Biosciences.All components must be mated only to their correct ports during system instal- lation. Power Strip/Surge Protector Setup Tum the power strip/surge protector power switch of.Plug the power strip/surge protector into a wall outlet (preferably a dedicated circuit)and tum the power on. CAUTION Do not plug the DE-500/DE-500 FC Multilmage ll light cabinet into the same power strip as the FluorChem system.Using a separate circuit is highly recommended.The cabinet must be tumed on after the computer's operating system has launched for the software to function properly. Computer,Monitor,Mouse,and Keyboard Setup The computer must have the monitor,mouse.and keyboard connected in the correct ports(see Figure I-1). Astandard three-pronoer cable shoud be plugedin the back of the computer and the power strip FluorChem HD2 and FC2 User Guide
FluorChem System Setup page 5 FluorChem HD2 and FC2 User Guide Hardware Installation All software, peripheral drivers, and operating systems come factory-installed on computers purchased directly from Cell Biosciences. All components must be mated only to their correct ports during system installation. Power Strip/Surge Protector Setup Turn the power strip/surge protector power switch off. Plug the power strip/surge protector into a wall outlet (preferably a dedicated circuit) and turn the power on. CAUTION Do not plug the DE-500/DE-500 FC MultiImage II light cabinet into the same power strip as the FluorChem system. Using a separate circuit is highly recommended. The cabinet must be turned on after the computer’s operating system has launched for the software to function properly. Computer, Monitor, Mouse, and Keyboard Setup The computer must have the monitor, mouse, and keyboard connected in the correct ports (see Figure 1-1). A standard three-prong power cable should be plugged into the back of the computer and the power strip