基因工程 Genetic Engineering
基因工程 Genetic Engineering
授课方式 We learn... 10%of what we read 20%o of what we hear 30%of what we see 50%of what we both see and hear 70%o of what is discussed with others Students each act as a piece 80%of what of the puzzle who together we experience personally make up a bigger picture 95%of what we teach to someone else! 6
6 授课方式
22335454 4 Materials 3g544 y Reading,Discussion Expert Expert Expert Expert Group 1 Group 2 Group 3 Group 4 Teaching Discussion V Answer questions Home Home Home Home Group A Group B Group C Group D Regular score Making the invisible visible -Reading and writing
4 Materials Reading, Discussion Teaching Discussion Answer questions Regular score Making the invisible visible - Reading and writing
第一章 基因工程的概念及发展
第一章 基因工程的概念及发展
内容 一、 基因工程的概念 二、基因工程的诞生和发展 三、基因工程的研究内容 四、基因工程的应用
一、基因工程的概念 二、基因工程的诞生和发展 三、基因工程的研究内容 四、基因工程的应用 内 容
一 基因工程的概念 > Gene engineering includes a range of techniques and methods used by scientists to control or modify genes, switch them off or move them between two unrelated species. 一般意义上的基因工程是指“重组DNA技术”或“基因 转移技术”,即:在体外将核酸分子插入病毒、质粒或 其它载体分子,构成遗传物质的新组合,并使之参入到 原先没有这类分子的寄主细胞内,而能持续稳定的繁殖。 (且不è. 1008)
一、基因工程的概念 Gene engineering includes a range of techniques and methods used by scientists to control or modify genes, switch them off or move them between two unrelated species. 一般意义上的基因工程是指“重组DNA技术”或“基因 转移技术” ,即:在体外将核酸分子插入病毒、质粒或 其它载体分子,构成遗传物质的新组合,并使之参入到 原先没有这类分子的寄主细胞内,而能持续稳定的繁殖。 (吴乃虎 ,1998)
基因工程图解: 分离外源基因 十 载体 ↓重组 重组DNA 转化或 转染 整合 →筛选 受体细胞 转化子
重组DNA 筛选 分离外源基因 载体 重组 转化子 整合 基因工程图解: 转化或 转染 受体细胞
Chromosomal DNA Chromosome T-DNA T-DNA Ti plasmid Crown gall Agrobacterium tumnefaciens Transformed plant cell (a) (b) (c) (d)
基因工程的四要素 1、悬綦因 sequence -regulatory sequences 2、 ble codon usage for the host or target organism 3、转化方法 -cell line or germ line transformation 4、 毫,依yoreukayot
基因工程的四要素 1、目的基因 2、载体 3、转化方法 4、受体 –expressed sequence –regulatory sequences –suitable codon usage for the host or target organism –prokaryote or eukaryote –cell line or germ line transformation
The terms“gene technology”,“genetic engineering”and“genetic modification” mean the same thing,and refer to one type of modern biotechnology
The terms “gene technology”, “genetic engineering” and “genetic modification” mean the same thing, and refer to one type of modern biotechnology