Water potential in the plant Course: Plant Physiology Lab Experimental Teaching Centre Of Life Sciences
Water potential in the plant Course: Plant Physiology Lab Experimental Teaching Centre Of Life Sciences
The way of water absorbed by plant ■ Diffusion concentration gradient 简单扩散 浓度梯度 Mass flow- pressure gradient 集流 压力梯度 Osmosis- water potential gradient 渗透作用 水势梯度
The way of water absorbed by plant Diffusion —— concentration gradient 简单扩散 浓度梯度 Mass flow —— pressure gradient 集流 压力梯度 Osmosis —— water potential gradient 渗透作用 水势梯度
Water Potential ■ Chemical potential -free energy per mole of substance Water Potential the chemical potential of water divided by the partial molal volume of water
Water Potential Chemical Potential ——free energy per mole of substance Water Potential —— the chemical potential of water divided by the partial molal volume of water w w w w w V V w 0 ( )
Cell Water Potential Factors: concentration, pressure and gravity yw=ys typ tyg Water enters the cell along a water potential gradient Yw=ys+y 适用于有液泡的细胞或细胞群
Cell Water Potential Factors: concentration, pressure and gravity Water enters the cell along a water potential gradient 适用于有液泡的细胞或细胞群 w s p g w s p
(A)Pure water (B)Solution contalnlng 0. 1 M sucrose 0.1 M Sucrose solution Pure water Y。=0MPa s=-0.244MPa y =OMPa YeLp+ 0-0.244MPa =0 MPa -0 244 MPa (C)Flacdd cell dropped Into sucrose solution (D)Concentration of sucrose Increased Flaccid cell Turgid cell Yp =0MPa P=0.488 MPa s=-0.732MPa 中=-0.732MPa Yw=-0732 MPa Y.E-0.244 MPa 0.3 M Sucrose Cell after equilibrium solution Cell after equilibrium Yw=-0732 MPa P =0MPa Yw=-0 244 MPa 华s=-0732MPa 5=-0.732MPa Is =-0.732 MPa 鄄p=w-ys=0MPa Y=-0.732MPa Ww-ps=0.488 MPa (E)Pressure applled to cell Applied pressure squeezes out half the water, thus doubling ys from -0.732 to-1464 MPa 0. 1 M Sucrose solution ce‖ in initial state Cell in final state Fw=-0.244MP w=-0.244MPa 中s=-0.732MPa 华s=-1.464MPa Yn= Yw= ys=0488 MPa p=平w-Y=1.22MPa
Experient ■质壁分离法—测定细胞渗透势 质壁分离和质壁分离复原现象可证明植物细胞是 渗透系统。 小液流法——测定细胞水势 要求:叶片在蔗糖溶液中浸没的时间内不时振荡? 蔗糖溶液配制过程中充分混匀?
质壁分离法——测定细胞渗透势 质壁分离和质壁分离复原现象可证明植物细胞是一 个渗透系统。 小液流法——测定细胞水势 要求:叶片在蔗糖溶液中浸没的时间内不时振荡? 蔗糖溶液配制过程中充分混匀? Experient
Thinking a As the soil dries, how to adjust water potential of the plant?
Thinking As the soil dries, how to adjust water potential of the plant?