1 2 3 456 -97.4 66.2- -45.0- -31.0- -21.5- -14.4 SDS gel Immunoblot (c)
WESTERN BLOT(Proteins) Nitrocellulose filter 1Separate protein molecules by gel electrophoresis. Place membrane on gel. Buffer 3 Use an electrical field to Power transfer proteins from supply the gel to membrane. Labeled molecu bound to antiboc 4 Use fluorescent or Antibo radioactively labeled 可 antibodies to identify proteins of interest. Wash away unbound antibodies. Protein bour to membran
一 Stained Immunoblot gel (b) (c)
Western blot技术要点： 用途：定性蛋白质 蛋白质表达水平 测定混合样品中某蛋白的分子量 抗体的特异性
Western blot 技术要点： 用途：定性蛋白质 蛋白质表达水平 测定混合样品中某蛋白的分子量 抗体的特异性
AtKP1抗体的特异性鉴定 Aa b c 123456B 205 205 130- *pET-AtKP1* 130 97 -97 66 66 43 31- 3 pET-KA* 20- -20 14.4 14.4
AtKP1抗体的特异性鉴定