材料与能源学院 School of Materials and Energy 《材料分子结构分析》 第七章核磁共振谱 Nuclear Magnetic Resonance Spectroscopy, NMR wvalp on moy 1aBiofianau 刘钰 电子科技大学 2021年2022年春季
第七章 核磁共振谱 刘 钰 电子科技大学 2021年-2022年 春季 《材料分子结构分析》 Nuclear Magnetic Resonance Spectroscopy， NMR
never forget how to dream 目录 ■7.1核磁共振概述 ■7.2核磁共振谱的基本原理 ■7.3核磁共振谱波谱仪 ■7.4样品的制备和测试 ■7.5核磁共振氢谱 ■7.6核磁共振氢谱解析 ■7.7核磁共振氢谱的应用 ■7.8核磁共振碳谱 ■7.9核磁共振碳谱的解析及应用 2
2 目录 ◼ 7.1 核磁共振概述 ◼ 7.2 核磁共振谱的基本原理 ◼ 7.3 核磁共振谱波谱仪 ◼ 7.4 样品的制备和测试 ◼ 7.5 核磁共振氢谱 ◼ 7.6 核磁共振氢谱解析 ◼ 7.7 核磁共振氢谱的应用 ◼ 7.8 核磁共振碳谱 ◼ 7.9 核磁共振碳谱的解析及应用
7.1核磁共振概述 never for fream 核磁共振的发展 1938 Discovery of nuclear magnetic resonance (NMR) 1946 Principle of solid state NMR(Bloch, Purcell) 1950 Resonance frequency depends on chemical environment(Proctor,Yu) 1953 Overhauser effect 1956 First NMR spectra of protein (Ribonuclease) 1965 Fourier Transform spectroscopy(Ernst) 3
3 核磁共振的发展 1946 Principle of solid state NMR (Bloch, Purcell) 1950 Resonance frequency depends on chemical environment (Proctor, Yu) 1953 Overhauser effect 1956 First NMR spectra of protein (Ribonuclease) 1965 Fourier Transform spectroscopy (Ernst) 1938 Discovery of nuclear magnetic resonance （NMR） 7.1 核磁共振概述
71核磁共振概述 neger forget 1973 Imaging tomography(Mansfieidj Sc2 TSEMM SL8 1985 First protein structure(bovine pancreatic trypsin inhibitor)in solution (Wuthrich) Figure 27.NMR structure of BPTI represented by a bundle of 20 con- formers superim- posed for best fit of the polypeptide backbone.The polypeptide back- bone is green,core side-chains are blue, and solvent-accessi- ble surface side- chains are red. 4
4 1985 First protein structure (bovine pancreatic trypsin inhibitor) in solution (Wüthrich) 1973 Imaging tomography (Mansfield) 7.1 核磁共振概述
never forget 7.1核磁共振概述 how to aream By now:More than 150 protein structures (M103 s-1 (>15s) >1048 1700s- Protein dynamics 300s1 5
5 By now: More than 150 protein structures (M < 60 000) BPTI Bound water Protein dynamics 7.1 核磁共振概述
7.1 核磁共振之还0 218 45 Functional MRI 73 145 218 1Mepn网 290 Study:50413 Stuy:50413 Series:603 ID:service Series:603 ID:service Date:May 31 2005 Image:95 Date:May 31 2005 leftmotor6x TR:0.00 Window:500 leftmotor6x TE:0.00 Level:200 Study:50413 Study:50413 Series:603 ID:service Series:603 ID:service Image:100 Date:May 31 2005 Image:167 Date:May 31 2005 leftmotor6x leftmotor6x TR:0.00 Window:500 TR:0.00 Window:500 T000 Level:200TE:0.00 Level:200
6 Functional MRI 7.1 核磁共振概述
never forget 7.1核磁共振概述 now to aream the heart aorta cross section left of the abdomen ventricle pancreas tibia knee joint the brain femur magnet gall bladder the spine bliary traot and the spinal canal pancre但tid duc and the spinal cord MRI is used for imaging of all organs in the body. 7
7 MRI is used for imaging of all organs in the body. 7.1 核磁共振概述
never forget 7.1核磁共振概述 how to aream NMR Nobel Prize Winners Another Nobel ■1944 Isador Rabi for Magnetic Resonance! Just before going to press we received the most memorable news of the year:The Nobel Prize in Physiology or Medicine ■1952 Felix Bloch for 2003 was awaded jointly to Paul C.Lauterbur (Uana IL) and Sir Peter Mansfield (Nottingham.UK)for their pioneering contributions which led to the application of magnetic Edwin Purcell resonance in medical imaging. The list of Nobel laureates in the MR field is impressive. beginning with Isador I.Rabi (Nobel Prize in Physics,1944) for his resonance method for recording the magnetic properties of atomic nuclei. The NMR phenomenon was demonstrated for protons in 1946 by Felix Bloch and Edward M.Purcell,USA.(Nobel Prize in Physics,1952).For his fundamental contributions to NMR ■1991 Richard Ernst methodology,Richard Ernst.Zirich,received the Nobel Prize in Chemistry,1991:and Kurt Wuthrich,Zunch,shared the Chemistry Prize,2002,for his development of NMR tochniques for determining the 3D structure of biological macromon ■2o02 Kurt Wuthrich solution. A further highlight was this year's Nobel Prize in Physics: Alexeij A.Abrikosow (Argonne,IL)and Vitalij L.Ginzburg (Moscow)were each awarded one-third of the prize for pioneering contributions to the theory of type-ll superconductors,i.e.,those alloys capable of withstanding the high magnetic fields that occur in MR applications. ■2003 Paul Lauterbur We at Bruker BioSpin and all members of the MR community are indebeed to these researchers for their contributions.We enthusiastically congratulate this year's laureates and take their achievements and recognition as further stimuli for our own cfforts Sir Peter Mansfield to improve MR instrumentation and cxpand MR applications. 8
8 NMR Nobel Prize Winners ◼ 1944 Isador Rabi ◼ 1952 Felix Bloch & Edwin Purcell ◼ 1991 Richard Ernst ◼ 2002 Kurt Wüthrich ◼ 2003 Paul Lauterbur & Sir Peter Mansfield 7.1 核磁共振概述
7.1核磁共振概述 never forget now to aream The Nobel Prize in Physics 1952 "for their development of new methods for nuclear magnetic precision measurements and discoveries in connection therewith Felix Bloch Edward Mills Purcell 9
9 The Nobel Prize in Physics 1952 "for their development of new methods for nuclear magnetic precision measurements and discoveries in connection therewith " Felix Bloch Edward Mills Purcell 7.1 核磁共振概述
7.1核磁共振概述 never forget now to aream The Nobel Prize in Chemistry 1991 "for his contributions to the development of the methodology of high resolution nuclear magnetic resonance(NMR)spectroscopy" Richard R.Ernst 10
10 The Nobel Prize in Chemistry 1991 "for his contributions to the development of the methodology of high resolution nuclear magnetic resonance (NMR) spectroscopy" Richard R. Ernst 7.1 核磁共振概述