《Pathogenic biology andImmunology Experiment)(for Six-year Term Clinical Medicine Specialty internationalstudents)TeachingprogramLaboratory of pathogenic biology and immunology,experimental teaching management centerOctober, 2021
1 《Pathogenic biology and Immunology Experiment》 (for Six-year Term Clinical Medicine Specialty international students) Teaching program Laboratory of pathogenic biology and immunology, experimental teaching management center October, 2021
Preface1、Basic informationCourse Name:pathogenic biologyand ImmunologyExperimentCredit hours: 64 credit hours in totalApplicable disciplines: Six-year Term Clinical Medicine Specialty (ForeignStudents)Prerequisite courses: pathogenic biology, medical Immology2、Course introductionPathogenic biology and immunology experiment is a basic skill training course forundergraduates majoring in basic medicine. Its purpose is to help students deepen theirunderstanding and understanding of theoretical knowledge, and cultivate students'experimental ideas, basic skills, aseptic concept, clinical skills, innovativeconsciousness and ability to participate in scientific research; Exercise students abilityto use theoretical knowledge to solve clinical practical problems; Lay a foundation forclinical medicine in the future. This syllabus is prepared according to the teachingrequirements of undergraduate students of basic medicine in medical colleges anduniversities, the arrangement of our teaching plan and the theoretical syllabus. The totalclass hours are 64 class hours and a total of 16 experiments. Among them, there are 16class hours for Medical Immunology (25%), 24 class hours for Human Parasitology(37.5%), and 24 class hours for medical microbiology (37.5%)3,Course objectives(1)ProfessionalqualityobjectivesThrough the study of this course, students will be trained to respect science, seektruth from facts, love medical career, be not afraid of hardship, and serve patients withgood medical professional quality.(2)KnowledgeobjectivesThrough the study of this course, students can master the basic characteristics ofpathogenic microorganisms and combine them with clinical manifestations. Under theguidance of teachers, they can design and complete the experiment according to the2
2 Preface 1、Basic information Course Name: pathogenic biology and Immunology Experiment Credit hours: 64 credit hours in total Applicable disciplines: Six-year Term Clinical Medicine Specialty (Foreign Students) Prerequisite courses: pathogenic biology，medical Immology 2、 Course introduction Pathogenic biology and immunology experiment is a basic skill training course for undergraduates majoring in basic medicine. Its purpose is to help students deepen their understanding and understanding of theoretical knowledge, and cultivate students' experimental ideas, basic skills, aseptic concept, clinical skills, innovative consciousness and ability to participate in scientific research; Exercise students' ability to use theoretical knowledge to solve clinical practical problems; Lay a foundation for clinical medicine in the future. This syllabus is prepared according to the teaching requirements of undergraduate students of basic medicine in medical colleges and universities, the arrangement of our teaching plan and the theoretical syllabus. The total class hours are 64 class hours and a total of 16 experiments. Among them, there are 16 class hours for Medical Immunology (25%), 24 class hours for Human Parasitology (37.5%), and 24 class hours for medical microbiology (37.5%). 3、Course objectives （1）Professional quality objectives Through the study of this course, students will be trained to respect science, seek truth from facts, love medical career, be not afraid of hardship, and serve patients with good medical professional quality. （2）Knowledge objectives Through the study of this course, students can master the basic characteristics of pathogenic microorganisms and combine them with clinical manifestations. Under the guidance of teachers, they can design and complete the experiment according to the
experimental knowledge of pathogenicbiology and immunology(3) Skill objectivesThroughthe study ofthis course,masterthe uniquebasic operation technology ofpathogenic biology and immunology experiment, and finally cultivate the studentsthinking ability of integrating theory with practice and basic with clinical, and have theability of independent learning, so as to lay a good foundation for the follow-up clinicalcourse learning.4.TeachingrequirementsAccording to the curriculum syllabus and objectives, ensure the achievement ofcurriculum teaching content, teaching process (links), knowledge and ability objectives,and cultivate students’autonomous learning ability.5.AssessmentmethodThe examination method of this course adopts the comprehensive evaluationmethod. Including attendance, daily homework, classroom performance, test, picturetest, etc.Participants: Chen Tingting,Deng Xichuan,Fang Ji, he Yonglin, Liu Jia, Liu QuanZhang GuangyuanAuthor: Chen TingtingReviewed by:HeYonglin3
3 experimental knowledge of pathogenic biology and immunology. （3）Skill objectives Through the study of this course, master the unique basic operation technology of pathogenic biology and immunology experiment, and finally cultivate the students' thinking ability of integrating theory with practice and basic with clinical, and have the ability of independent learning, so as to lay a good foundation for the follow-up clinical course learning. 4. Teaching requirements According to the curriculum syllabus and objectives, ensure the achievement of curriculum teaching content, teaching process (links), knowledge and ability objectives, and cultivate students' autonomous learning ability. 5. Assessment method The examination method of this course adopts the comprehensive evaluation method. Including attendance, daily homework, classroom performance, test, picture test, etc. Participants: Chen Tingting, Deng Xichuan, Fang Ji, he Yonglin, Liu Jia, Liu Quan, Zhang Guangyuan Author: Chen Tingting Reviewed by: He Yonglin
Referencebook1. Jawetz,Melnick and Adelberg's. Medical Microbiology.27th Edition. TheMcGraw-HillCompanies.20162.Harley-Prescott. Laboratory Exercises in Microbiology, 5th Edition. The McGraw-Hill Companies.20024
4 Reference book 1. Jawetz,Melnick and Adelberg’s. Medical Microbiology.27th Edition. The McGraw-Hill Companies. 2016. 2. Harley-Prescott. Laboratory Exercises in Microbiology, 5th Edition. The McGrawHill Companies. 2002
CatalogueExperiment 1Determinationofphagocytosisof neutrophilsbybacterial counting7Experiment 2 Agglutination test..8Experiment3Immunolabelingtechnology.Experiment4Dotenzymeimmunoassay8Experiment 5 Observation of insect eggs and common objects easily confused withinsect eggs in feces.910Experiment 6 Medical nematodesExperiment 7 Medical fluke10.11Experiment 8 Medical tapeworm..12Experiment 9 Medical protozoa.13Experiment 10 Medical arthropods..14Experiment 1lObservation ofbacterial morphology and structure...14Experiment 12Artificial culture,disinfection and sterilization of bacteria...15Experiment 13 Pyogenic cocci.16xExperiment 14 Enterobacte.Experiment 15Anaerobic bacteria and Mycobacterium tuberculosis1617Experiment16Fungi,virusesand othermicrobe
5 Catalogue Experiment 1 Determination of phagocytosis of neutrophils by bacterial counting 7 Experiment 2 Agglutination test.7 Experiment 3 Immunolabeling technology.8 Experiment 4 Dot enzyme immunoassay.8 Experiment 5 Observation of insect eggs and common objects easily confused with insect eggs in feces.9 Experiment 6 Medical nematodes.10 Experiment 7 Medical fluke.10 Experiment 8 Medical tapeworm.11 Experiment 9 Medical protozoa.12 Experiment 10 Medical arthropods.13 Experiment 11 Observation of bacterial morphology and structure.14 Experiment 12 Artificial culture, disinfection and sterilization of bacteria.14 Experiment 13 Pyogenic cocci.15 Experiment 14 Enterobacte.16× Experiment 15 Anaerobic bacteria and Mycobacterium tuberculosis.16 Experiment 16 Fungi, viruses and other microbe.17
Teaching hours allocation table (64 class hours in total)ExperimentalType ofExperiment No.Experimenttitleclass hoursexperimentExperiment 1 Determination of phagocytosis of neutrophils4Comprehensiveby bacterial counting4Basic methodExperiment 2 Agglutination test4ComprehensiveExperiment 3 Immunolabeling technology4VerificationExperiment 4 Dot enzyme immunoassayExperiment 5 Observation of insect eggs and common4objects easily confused with insect eggs inBasic methodfeces4Experiment 6 Medical nematodesComprehensive4ComprehensiveExperiment 7Medical fluke4Experiment 8 Medical tapewormComprehensive4Experiment 9 Medical protozoaComprehensive4ComprehensiveExperiment 10 Medical arthropodsExperiment 11 Observation of bacterial morphology and4Basic methodstructure12ArtificialandExperimentculture,disinfection4Verificationsterilization of bacteria4.Experiment 13 Pyogenic cocciComprehensive4Experiment 14 EnterobacteComprehensiveExperiment 15 Anaerobic bacteria and Mycobacterium4Comprehensivetuberculosis4Experiment 16Fungi,viruses and other microbesComprehensive64合计6
6 Teaching hours allocation table (64 class hours in total) Experiment No. Experiment title Experimental class hours Type of experiment Experiment 1 Determination of phagocytosis of neutrophils by bacterial counting 4 Comprehensive Experiment 2 Agglutination test 4 Basic method Experiment 3 Immunolabeling technology 4 Comprehensive Experiment 4 Dot enzyme immunoassay 4 Verification Experiment 5 Observation of insect eggs and common objects easily confused with insect eggs in feces 4 Basic method Experiment 6 Medical nematodes 4 Comprehensive Experiment 7 Medical fluke 4 Comprehensive Experiment 8 Medical tapeworm 4 Comprehensive Experiment 9 Medical protozoa 4 Comprehensive Experiment 10 Medical arthropods 4 Comprehensive Experiment 11 Observation of bacterial morphology and structure 4 Basic method Experiment 12 Artificial culture, disinfection and sterilization of bacteria 4 Verification Experiment 13 Pyogenic cocci 4 Comprehensive Experiment 14 Enterobacte 4 Comprehensive Experiment 15 Anaerobic bacteria and Mycobacterium tuberculosis 4 Comprehensive Experiment 16 Fungi ,viruses and other microbes 4 Comprehensive 合 计 64
Experiment 1ThePhagocytic Function of Neutrophilswas MeasuredByBacterial Counting[Type of experiment]Comprehensive experiment[Purpose requirements]1. Master the principle, method and application of bacterial counting method formeasuring neutrophil phagocytosis.2. Be familiar with the biological characteristics and significance of neutrophils3. Understand the types, principles and methods of neutrophil phagocytosis test.[Experimental hours]4 class hours.[Experiment content]1. The phagocytic function of neutrophils was measured by bacterial count2. To investigate the effect of norepinephrine on neutrophil phagocytosis in vitro.[Experimental grouping]3~4persons/groupExperiment2AgglutinationTestType of experiment)Basic method experiments.[Purpose requirements]1. Master the characteristics and influencing factors of in vitro antigen antibodyreaction, the types of classical in vitro antigen antibody reaction, the principle andapplication of slide agglutination test.2. Be familiar with the type, characteristics and application of agglutination test;Familiar with the identification method of ABO blood group, Familiar with theprinciple and application of indirect latex agglutination test, Be familiar with the7
7 Experiment 1 The Phagocytic Function of Neutrophils was Measured By Bacterial Counting [Type of experiment] Comprehensive experiment. [Purpose requirements] 1. Master the principle, method and application of bacterial counting method for measuring neutrophil phagocytosis. 2. Be familiar with the biological characteristics and significance of neutrophils. 3. Understand the types, principles and methods of neutrophil phagocytosis test. [Experimental hours] 4 class hours. [Experiment content] 1. The phagocytic function of neutrophils was measured by bacterial count. 2. To investigate the effect of norepinephrine on neutrophil phagocytosis in vitro. [Experimental grouping] 3 ~ 4 persons / group. Experiment 2 Agglutination Test Type of experiment] Basic method experiments. [Purpose requirements] 1. Master the characteristics and influencing factors of in vitro antigen antibody reaction, the types of classical in vitro antigen antibody reaction, the principle and application of slide agglutination test. 2. Be familiar with the type, characteristics and application of agglutination test; Familiar with the identification method of ABO blood group; Familiar with the principle and application of indirect latex agglutination test; Be familiar with the
method and result judgment of rheumatoid factor detection experiment3. Understand tube agglutination test and precipitation reaction[Experimental hours]4 class hours.[Experiment content]1. Slide agglutination test (blood group identification)2. Indirect latex agglutination test (detection of rheumatoid factor)[Experimental grouping]3 ~ 4 persons / group.Experiment3ImmunolabelingTechnique[Type of experiment]Comprehensiveexperiment.[Purpose requirements]1. Master the type, characteristics and application ofELISA; Master the basic stepsand result judgment of double antibody sandwich ELISAfor detecting HBsAg2. Be familiar with the basic principle, characteristics, types and applications ofenzyme immunoassay; Be familiar with the principle and method of colloidal gold dotchromatography for detecting early pregnancy3. Understand the basic principle, types, characteristics and application ofimmunolabeling technology.[Experimental hours]4 class hours.[Experiment content]1.Video:immunolabeling technology2.HBsAg Was detected by double antibody sandwich ELISA3. Colloidal gold test paper to detect urine HCG (early pregnancy detection)[Experimental grouping]3 ~ 4 persons / group.8
8 method and result judgment of rheumatoid factor detection experiment. 3. Understand tube agglutination test and precipitation reaction. [Experimental hours] 4 class hours. [Experiment content] 1. Slide agglutination test (blood group identification). 2. Indirect latex agglutination test (detection of rheumatoid factor). [Experimental grouping] 3 ~ 4 persons / group. Experiment 3 Immunolabeling Technique [Type of experiment] Comprehensive experiment. [Purpose requirements] 1. Master the type, characteristics and application of ELISA; Master the basic steps and result judgment of double antibody sandwich ELISA for detecting HBsAg. 2. Be familiar with the basic principle, characteristics, types and applications of enzyme immunoassay; Be familiar with the principle and method of colloidal gold dot chromatography for detecting early pregnancy. 3. Understand the basic principle, types, characteristics and application of immunolabeling technology. [Experimental hours] 4 class hours. [Experiment content] 1. Video: immunolabeling technology. 2. HBsAg was detected by double antibody sandwich ELISA. 3. Colloidal gold test paper to detect urine HCG (early pregnancy detection). [Experimental grouping] 3 ~ 4 persons / group
Experiment4DotEnzymeImmunoassay[Type of experiment]Confirmatory experiment.[Purpose requirements]1. Master the basic principle, characteristics and application of dot enzymeimmunoassay.2.Befamiliar with themethods and steps of dot enzyme immunoassay fordetecting trace antigens.3.Understand thequantitative analysis of dotenzyme immunoassay results[Experimental hours]3 class hours.[Experiment content]1. Dot enzyme immunoassay (dot ELISA) was used to detect IL-2[Experimental grouping]3 ~ 4 persons / groupExperiment5Observation on Insect eggsin Feces andCommonObjectsEasilyConfusedwithInsectEggs[Type of experiment]Basicmethod experiment.[Purpose requirements]1. Master the operation of common fecal examination methods such as fecal directsmear method.2.Be familiar with the difference between parasites or parasite eggs in feces andother shape analogues.[Experimental hours]4 class hours[Experiment content]1. The eggs of intestinal parasitic nematodes were observed by fecal direct smearmethod9
9 Experiment 4 Dot Enzyme Immunoassay [Type of experiment] Confirmatory experiment. [Purpose requirements] 1. Master the basic principle, characteristics and application of dot enzyme immunoassay. 2. Be familiar with the methods and steps of dot enzyme immunoassay for detecting trace antigens. 3. Understand the quantitative analysis of dot enzyme immunoassay results. [Experimental hours] 3 class hours. [Experiment content] 1. Dot enzyme immunoassay (dot ELISA) was used to detect IL-2. [Experimental grouping] 3 ~ 4 persons / group Experiment 5 Observation on Insect eggs in Feces and Common Objects Easily Confused with Insect Eggs [Type of experiment] Basic method experiment. [Purpose requirements] 1. Master the operation of common fecal examination methods such as fecal direct smear method. 2. Be familiar with the difference between parasites or parasite eggs in feces and other shape analogues. [Experimental hours] 4 class hours. [Experiment content] 1. The eggs of intestinal parasitic nematodes were observed by fecal direct smear method
2. Specimen observation: starch, pollen, oil droplets, bubbles, plant cells, animalcells and plant fibers in feces.[Experimental grouping]3-4 persons / group.Experiment6Medical Nematodes[Type of experiment]Comprehensive experiment.[Purpose requirements]1.Master the morphological characteristics of Ascaris lumbricoides eggs,whipworm eggs, hookworm eggs, pinworm eggs, bancrofi and Malay microfilariae,and Trichinella spiralis cysts2.Befamiliar with the adult morphology of Ascaris lumbricoides, whipworm,hookworm, pinworm, filariasis and Trichinella spiralis[Experimental hours]4 class hours.[Experiment content]1.Smear or patch observation:Ascaris lumbricoides eggs (fertilized eggs,unfertilized eggs, deproteinized eggs, infectious Ascaris lumbricoides eggs), whipwormeggs, hookworm eggs suspension smears, pinworm eggs patch specimens, and oral sacof two kinds of hookworm.2.Gross specimen observation:Ascaris lumbricoides, whipworm, hookworm,pinworm adult, bancrofi microfilaria, Malay microfilaria, Trichinella spiralis cyst.Trichinella spiralis adult, filariasis, Trichinella spiralis adult parasitic on diseasedorgans and tissues of humanbody[Experimental grouping]3-4 persons / group.Experiment7MedicalFluke10
10 2. Specimen observation: starch, pollen, oil droplets, bubbles, plant cells, animal cells and plant fibers in feces. [Experimental grouping] 3-4 persons / group. Experiment 6 Medical Nematodes [Type of experiment] Comprehensive experiment. [Purpose requirements] 1. Master the morphological characteristics of Ascaris lumbricoides eggs, whipworm eggs, hookworm eggs, pinworm eggs, bancrofti and Malay microfilariae, and Trichinella spiralis cysts. 2. Be familiar with the adult morphology of Ascaris lumbricoides, whipworm, hookworm, pinworm, filariasis and Trichinella spiralis. [Experimental hours] 4 class hours. [Experiment content] 1. Smear or patch observation: Ascaris lumbricoides eggs (fertilized eggs, unfertilized eggs, deproteinized eggs, infectious Ascaris lumbricoides eggs), whipworm eggs, hookworm eggs suspension smears, pinworm eggs patch specimens, and oral sac of two kinds of hookworm. 2. Gross specimen observation: Ascaris lumbricoides, whipworm, hookworm, pinworm adult, bancrofti microfilaria, Malay microfilaria, Trichinella spiralis cyst, Trichinella spiralis adult, filariasis, Trichinella spiralis adult parasitic on diseased organs and tissues of human body. [Experimental grouping] 3-4 persons / group. Experiment 7 Medical Fluke