wGI©omE htlp://www.365pic.com
welcome
Introduction of Multi Myograph System 610M ▣2日▣四 Wu Liping 2004-6-4
Wu Liping 2004-6-4
Background The technique was first developed for investigating small vessels with internal diameters down to around 100 um by Mulcany Halpern in 1976,1977 Multi Myograph System -610M is suited to vessels with internal diameters of 100-400 um At least 50%of the precapillary pressure drop occurs in the vessels with internal diameters than 100 um proximal resistance vessels)
Background ➢ The technique was first developed for investigating small vessels with internal diameters down to around 100 μm by Mulcany & Halpern , in 1976,1977 ➢ Multi Myograph System – 610M is suited to vessels with internal diameters of 100-400 μm ➢ At least 50% of the precapillary pressure drop occurs in the vessels with internal diameters than 100 μm ( proximal resistance vessels)
General Principles Opening order: powerlab myograph software Closing order: software→myograph→powerlab Line connection: one-to-one correspondingly
General Principles ➢ Opening order: powerlab myograph software ➢ Closing order: software myograph powerlab ➢ Line connection: one-to-one correspondingly
General Principles Buttons'function: 凸：main options (zero/calibrate/temperature,etc.) number changing (decrease/increase) F1 channel selecting F2 detailed operation
General Principles ➢ Buttons’ function: • : main options (zero/calibrate/temperature, etc.) • : number changing (decrease/increase) • F1 : channel selecting • F2 : detailed operation
General Principles 2g weight Panr网 Calibration bridge Pan amm -LoadW tip Load =W wire Zero before ue jsnpsueL experiments: Force Force F=9.81mN fig.5 fig.6
General Principles ➢ Zero before experiments: F = 9.81 m N 2 g weight Calibration bridge tip wire
solutions MW PSS2.5 K-PSS2.s g/mol mmol/l g/l mmol/I g NaCl 58.44 119 6.954 0 0 KCI 74.56 4.7 0.35 123.7 9.223 CaCl2.2H20 147.02 2.5 0.368 2.5 0.368 MgS04.7H20 246.68 1.17 0.289 1.17 0.289 NaHCO3 84.01 25 2.1 25 2.1 KH2P04 136.1 1.18 0.161 1.18 0.161 EDTA 372.24 0.027 0.010 0.027 0.010 Glucose 198.77 5.5 1.091 5.5 1.091 NAK:KPSS+NE 10 uM NAPSS:PSS+NE 10 uM
solutions MW PSS2.5 K-PSS2.5 g/mol mmol/l g/l mmol/l g/l NaCl 58.44 119 6.954 0 0 KCl 74.56 4.7 0.35 123.7 9.223 CaCl2●2H2O 147.02 2.5 0.368 2.5 0.368 MgSO4●7H2O 246.68 1.17 0.289 1.17 0.289 NaHCO3 84.01 25 2.1 25 2.1 KH2PO4 136.1 1.18 0.161 1.18 0.161 EDTA 372.24 0.027 0.010 0.027 0.010 Glucose 198.77 5.5 1.091 5.5 1.091 NAK: KPSS+NE 10 μ M NAPSS: PSS+NE 10 μM
dissection Example The dissection of 'excised arcade" a mesenteric small ateries Proximal Identifying cut from rats at autopsy distal mid-line laparotomy to exteriorize mesenteric bed Use scissors to remove 10cm of intestine together with its feeding Place excised section in Petri dish vasculature,including part of the containing PSS.Petri dish contains superior mesenteric artery. a 5 mm thick layer of Sylgaard to Proximal end of intestinal section hold fixing pins. should be 10cm from pylorus
dissection ➢ The dissection of mesenteric small ateries from rats at autopsy • mid-line laparotomy to exteriorize mesenteric bed • Use scissors to remove 10cm of intestine together with its feeding vasculature, including part of the superior mesenteric artery. Proximal end of intestinal section should be 10cm from pylorus Example • Place excised section in Petri dish containing PSS. Petri dish contains a 5 mm thick layer of Sylgaard to hold fixing pins
dissection "excised arcade a Proximal Pin down the proximal end of the Identifying cut intestine on the left hand side of the distal dish,and pin out the remainder of the intestine in an anticlockwise direction Using ocular dissection scissors, .Dissect out the test segment cut through the mesenteric (normally the third branch)together membrane along either side of with a piece of the proximal branch vessel,about 1-2mm from the vessel
dissection • Pin down the proximal end of the intestine on the left hand side of the dish, and pin out the remainder of the intestine in an anticlockwise direction •Dissect out the test segment (normally the third branch) together with a piece of the proximal branch • Using ocular dissection scissors, cut through the mesenteric membrane along either side of vessel, about 1-2mm from the vessel
dissection Dissect away the vein artery (distinguish from the pattern of the branch points) note "V" Clean artery of connective tissue by holding the connective tissue with forceps and pulling gently vein away from the artery note,no"V" Cut distal end of vessel to be forceps investigated and cut unwanted arcade 2mm from feeder artery, adipose tissue which are to be used as a "handle scissors to move the vessel Cut proximal end of feeder artery membrane
dissection • Dissect away the vein (distinguish from the pattern of the branch points) • Clean artery of connective tissue by holding the connective tissue with forceps and pulling gently away from the artery • Cut distal end of vessel to be investigated and cut unwanted arcade 2mm from feeder artery, which are to be used as a “handle” to move the vessel • Cut proximal end of feeder artery