Involvement of the Mitochondrial Calcium Uniporter in Cardioprotection by Ischemic Preconditioning
Involvement of the Mitochondrial Calcium Uniporter in Cardioprotection by Ischemic Preconditioning
Background Ischemic preconditioning (IPC. which was originally identified by Murry et al.)provides powerful I protection to the ischemic myocardium.Although a variety of intracellular signaling pathways have been implicated in this protection,the precise mechanisms remain elusive
Background Ischemic preconditioning (IPC, which was originally identified by Murry et al.) provides powerful protection to the ischemic myocardium. Although a variety of intracellular signaling pathways have been implicated in this protection, the precise mechanisms remain elusive
80 An important 70 8 PC phenomenon: Diazoxide 60 PC.5HO Cortrol The close ◆ Diazoxide+5HD 50 relationship 40 among IPC,the mitochondrial 30 calcium 20 concentration and 10 500 100e 1500 2000 2500 3000 3500 the improved [Ca2](nM) contractile function of heart
An important phenomenon: The close relationship among IPC , the mitochondrial calcium concentration and the improved contractile function of heart
A guess There might be certain relationship between ischemic preconditioning and calcium transport system of mitochondrial
A guess There might be certain relationship between ischemic preconditioning and calcium transport system of mitochondrial
Mitochondrial permeability transition pore(MPTP)and ischemic preconditioning mCU Ca2+ 3Na+ H+ Ca2+ △pllm H AYin Ca2+ 2H+ NICE e.t.c
Mitochondrial permeability transition pore (MPTP) and ischemic preconditioning
Local [Ca2+]control between Mitochondrial SR and mitochondria calcium SR SR Ca buffers 0000 uniporter plays SERCA important role SR-mito cleft VDAC in mediating OMM the enter of ntermembrane space calcium into Mitochondrial Cabuffers the IMM mitochondrial. Mitochondrial matrix
Mitochondrial calcium uniporter plays important role in mediating the enter of calcium into the mitochondrial
Questions: 1.Whether activity of mitochondrial calcium uniporter is involved in ischemic preconditioning? 2.And how the relationship between the mitochondrial calcium uniporter and the mitochondrial permeability transition pore?
Questions: 1.Whether activity of mitochondrial calcium uniporter is involved in ischemic preconditioning? 2.And how the relationship between the mitochondrial calcium uniporter and the mitochondrial permeability transition pore?
Part one:Isolated heart experiment Perfus ion fluid (37c,95%02, 5%C0以 Apparatus Heat exchanger Chart records Contractile force Heart rate CBF Tension transducer Amplifier ■ Perfusion Fluid Heart pressure rate meter control Drop Counter MENU
Part one: Isolated heart experiment
Time (min) 051020 5060 170 TT R Perfusion IPC IPC 围 protocols used for RR RR-Pre hemodynamic Ru360 Ru360-Pre measurements in RR isolated rat hearts RR Sper Sper A IPC+Sper 围 IPC+Sper+CsA CsA
Perfusion protocols used for hemodynamic measurements in isolated rat hearts
Contractile function investigation: Left ventricular developed pressure (LVDP) Left ventricular end diastolic pressure (LVEDP) +dP/dtmax (the maximal left ventricular pressure increase rate .-dP/dtmax (the maximal left ventricular pressure decrease rate)
Contractile function investigation: • Left ventricular developed pressure (LVDP) • Left ventricular end diastolic pressure (LVEDP) • +dP/dtmax (the maximal left ventricular pressure increase rate) • - dP/dtmax (the maximal left ventricular pressure decrease rate)