《分子生物学D》（供六年制临床医学专业来华留学生使用）教学大纲基础医学院生物化学与分子生物学教研室2021年10月
《分子生物学 D》 （供六年制临床医学专业来华留学生使用） 教 学 大 纲 基础医学院生物化学与分子生物学教研室 2021 年 10 月
Preface1.BasicinformtionName:Molecular BiologyClass hours: 36 total class hours including 27 teaching hours and 9 experimental hours.2 total credit hours.Speciality:six-year termclinical medicine specialty (foreign students)The prerequisite courses: Biology, Chemistry, Organic Chemistry and Biochemistry2.IntroductionIn a broad viewpoint, Molecular Biology concerns the understanding of biologicalphenomena in molecular terms. More restrictively, Molecular Biology refers to the study ofgene structure and function at the molecular level. Just like Biochemistry, MolecularBiology has also become the basic language of all life sciences. A thorough comprehensionof Molecular Biology will enable students to best understand the molecular basis ofdisease.Molecular Biology is an important and foundamental preclinical course for advancedmedial education. Molecular Biology lays the foundation for other basic and clinicalmedical sciences.3.ObjecttivesThe Molecular Biology course is taught at the fourth semester for internationalstudents majoring clinical medicine. This course introduces the foundations of moleculabiology and provides medical perspectives. Through this ourse, students could understandthe central dogma and the common molecular technologies and methods. Through thiscourse study, students will understand the basis of disease developement and try to explorethemolecularmechnism of somediseases.4.RequimentsThe recommended textbook is Lippincott's Illustrated Reviews: Biochemistry (6thedition). This teaching outline is mainly constituted based on China National MedicalLicensing Examination (CNMLE) and United States Medical Licensing Examination(USMLE). The teaching aims and requirements for each chapter are mainly set up at three
Preface 1. Basic informtion Name: Molecular Biology Class hours: 36 total class hours including 27 teaching hours and 9 experimental hours. 2 total credit hours. Speciality: six-year term clinical medicine specialty (foreign students) The prerequisite courses: Biology, Chemistry, Organic Chemistry and Biochemistry. 2. Introduction In a broad viewpoint, Molecular Biology concerns the understanding of biological phenomena in molecular terms. More restrictively, Molecular Biology refers to the study of gene structure and function at the molecular level. Just like Biochemistry, Molecular Biology has also become the basic language of all life sciences. A thorough comprehension of Molecular Biology will enable students to best understand the molecular basis of disease. Molecular Biology is an important and foundamental preclinical course for advanced medial education. Molecular Biology lays the foundation for other basic and clinical medical sciences. 3. Objecttives The Molecular Biology course is taught at the fourth semester for international students majoring clinical medicine. This course introduces the foundations of molecular biology and provides medical perspectives. Through this ourse, students could understand the central dogma and the common molecular technologies and methods. Through this course study, students will understand the basis of disease developement and try to explore the molecular mechnism of some diseases. 4. Requiments The recommended textbook is Lippincott's Illustrated Reviews: Biochemistry (6th edition). This teaching outline is mainly constituted based on China National Medical Licensing Examination (CNMLE) and United States Medical Licensing Examination (USMLE). The teaching aims and requirements for each chapter are mainly set up at three
differentlevels(Understand>Befamiliar with>Beawareof)5.MethodsThis course will be taught mainly by Lecture with Multimedia. Students can learn partofchapterbyself-studyorgroupdiscussion6.EvaluationWeiEvaluationEvaluationEvaluation standardsItemghtabilityindicatorOnline learning activity in Umooc:E-learningSelf-studyPrepare(100 points)ability,1. Online length (5%) (100 points):lessionby33.3Analyzing-pro≥100 minutes, 100 points; <100, 11. Online lengthwatchingblem ability,points per time%2NumberofvideosandSolving-probl2.Numberofvideos/PPTsofcoursePPTsvideos/PPTs ofem ability(5%) (100 points) :≥10 times, 100course<10,0pointspoints,AnalyzingOnlineMasterand33.4andsolvingTests/quizzes in Umooc.useoftests/quizzes%problemAverage of these tests scores.Formatiknowledge(100 points )abilityveCorrectevaluatiexperimentalAnalyzingonandoperationsolving(E-learExperimentsandhomeworksinExperiments andandseriousproblemning and33.3Umoochomeworksexperimentalability,tests)%Two experimental reports, and I~2report,experimental(30%)(100 points )homeworks.Average of these scores.MasterandoperationUMOOCskills.useofknowledgeTake part in the course discussion areaPostand in Umooc:replyAnalyzingBonusNumber of posts ≥10 times, 3 points;questions inandsolvingtheproblemcourse(6 points)1~9 times, 2 pointsdiscussionabilityNumber of replies ≥10 times, 3 points;area1~9 times, 2 pointsSummarizedevaluatiAnalyzingonMasterand100Final examandsolving(FinaluseofFinal exam online(100 points)%problemexam)knowledgeability(70%)ExamonlineParticipant:Xianjun Liu, Faping Yi, Xue Jiang,Youquan BuWriter:Huifang ZhuAssessor:YouquanBu
different levels (Understand > Be familiar with > Be aware of). 5. Methods This course will be taught mainly by Lecture with Multimedia. Students can learn part of chapter by self-study or group discussion. 6. Evaluation Participant：Xianjun Liu, Faping Yi, Xue Jiang, Youquan Bu Writer：Huifang Zhu Assessor：Youquan Bu Item Wei ght Evaluation indicator Evaluation ability Evaluation standards Formati ve evaluati on （E-lear ning and tests） （30%） UMOOC E-learning (100 points) 1. Online length 2 Number of videos/PPTs of course 33.3 % Prepare lession by watching videos and PPTs Self-study ability, Analyzing-pro blem ability, Solving-probl em ability Online learning activity in Umooc： 1. Online length (5%) (100 points): ≥100 minutes, 100 points; <100, 1 points per time 2. Number of videos/PPTs of course (5%) (100 points) : ≥10 times, 100 points; <10, 0 points Online tests/quizzes (100 points ) 33.4 % Master and use of knowledge Analyzing and solving problem ability Tests/quizzes in Umooc. Average of these tests scores. Experiments and homeworks (100 points ) 33.3 % Correct experimental operation and serious experimental report, Master and use of knowledge Analyzing and solving problem ability, experimental operation skills. Experiments and homeworks in Umooc: Two experimental reports, and 1~2 homeworks. Average of these scores. Bonus (6 points) Post and reply questions in the course discussion area Analyzing and solving problem ability Take part in the course discussion area in Umooc: Number of posts ≥10 times, 3 points; 1~9 times, 2 points Number of replies ≥10 times, 3 points; 1~9 times, 2 points Summar ized evaluati on （Final exam） （70%） Exam online Final exam (100 points ) 100 % Master and use of knowledge Analyzing and solving problem ability Final exam online
Textbook, Teaching auxiliary books and BibliographyThis teaching outline is mainly constituted based on China National MedicalLicensing Examination (CNMLE) and United States Medical Licensing Examination(USMLE). So we choose Lippincott's Illustrated Reviews: Biochemistry (6th edition), aclassic common book as the textbook.1.TextbookDenise R. Ferrier. Lippincott's Illustrated Reviews: Biochemistry (6th).Baltimore:WoltersKluwer, Lippincott Williams&Wilkins,2014.2.TeachingauxiliarybooksYouquan Bu. Molecular Biology, Learning Guidelines & Self-Tests. (unpublishedbook)3.Bibliography(I) Michael A. Lieberman, Rick Ricer. Lippincott's illustrated Q & A review ofbiochemistry, 1st ed.Baltimore: Lippincott Williams & Wilkins. 2010(2) Michael A.Lieberman, Rick Ricer.BRS Biochemistry, Molecular Biology, andGenetics.Sixth Edition.Baltimore: Lippincott Williams & Wilkins.2014.(3) David L. Nelson, Michael M. Cox. Lehninger Principles of Biochemistry. 6th edNew York: W.H.Freeman and Company, 2013.(4) James D.Watson, Tania A.Baker, Stephen P. Bell, Alexander Gann, MichaelLevine, Richard Losick. Molecular Biology of the Gene. 7th ed.New York: Cold SpringHarbor Laboratory Press, 2013.(5) Victor W.Rodwell, David A. Bender, Kathleen M. Botham, Peter J. Kennelly, P.Anthony Weil. Harper's illustrated biochemistry. 30th ed. 2015. New York. McGraw-HillCompanies.(6)RobertF.Weaver.MolecularBiology,fifthedition.2012.NewYork.McGraw-HillCompanies
Textbook, Teaching auxiliary books and Bibliography This teaching outline is mainly constituted based on China National Medical Licensing Examination (CNMLE) and United States Medical Licensing Examination (USMLE). So we choose Lippincott's Illustrated Reviews: Biochemistry (6th edition), a classic common book as the textbook. 1. Textbook Denise R. Ferrier. Lippincott's Illustrated Reviews: Biochemistry (6th). Baltimore: Wolters Kluwer, Lippincott Williams &Wilkins, 2014. 2. Teaching auxiliary books Youquan Bu. Molecular Biology, Learning Guidelines & Self-Tests. (unpublished book) 3. Bibliography (1) Michael A. Lieberman, Rick Ricer. Lippincott’s illustrated Q & A review of biochemistry, 1st ed. Baltimore: Lippincott Williams & Wilkins. 2010. (2) Michael A. Lieberman, Rick Ricer. BRS Biochemistry, Molecular Biology, and Genetics. Sixth Edition. Baltimore: Lippincott Williams & Wilkins. 2014. (3) David L. Nelson, Michael M. Cox. Lehninger Principles of Biochemistry. 6th ed. New York: W. H. Freeman and Company, 2013. (4) James D. Watson, Tania A. Baker, Stephen P. Bell, Alexander Gann, Michael Levine, Richard Losick. Molecular Biology of the Gene. 7th ed. New York: Cold Spring Harbor Laboratory Press, 2013. (5) Victor W. Rodwell, David A. Bender, Kathleen M. Botham, Peter J. Kennelly, P. Anthony Weil. Harper's illustrated biochemistry. 30th ed. 2015. New York. McGraw-Hill Companies. (6) Robert F. Weaver. Molecular Biology, fifth edition. 2012. New York. McGraw-Hill Companies
OutlinesTeaching Arrangements (36 class hours)2IntroductionChapter 1 DNA Structure, Replication, and Repair .Chapter 2 RNA Structure, Synthesis, and Processing..Chapter3Protein Synthesis.4Chapter 4 Regulation of Gene Expression....5Chapter 5Biotechnology and Human Disease..6dExperiment1PlasmidExtractionExperiment 2 PCR......8Appendix:ClinicalCase study
Outlines Teaching Arrangements (36 class hours).1 Introduction .2 Chapter 1 DNA Structure, Replication, and Repair .2 Chapter 2 RNA Structure, Synthesis, and Processing.3 Chapter 3 Protein Synthesis .4 Chapter 4 Regulation of Gene Expression.5 Chapter 5 Biotechnology and Human Disease.6 Experiment 1 Plasmid Extraction .8 Experiment 2 PCR.8 Appendix: Clinical Case study .9
Teaching Arrangements (36 class hours)TeachingExperimentTeachingContentsHoursHours1Introduction5Chapter 1 DNA Structure, Replication, and Repair3Chapter 2 RNA Structure, Synthesis, and Processing6Chapter 3 Protein Synthesis6Chapter4RegulationofGeneExpression6Chapter 5 Biotechnology and Human Disease4Experiment1PlasmidExtraction5Experiment 2 PCR927In total1
1 Teaching Arrangements (36 class hours) Teaching Contents Teaching Hours Experiment Hours Introduction 1 Chapter 1 DNA Structure, Replication, and Repair 5 Chapter 2 RNA Structure, Synthesis, and Processing 3 Chapter 3 Protein Synthesis 6 Chapter 4 Regulation of Gene Expression 6 Chapter 5 Biotechnology and Human Disease 6 Experiment 1 Plasmid Extraction 4 Experiment 2 PCR 5 In total 27 9
Introduction1.Objectives &Requirements() Understand the definition and the significance of the central dogma(2) Be familiar with the definition of molecular biology, gene, genome, andproteomics.(3) Be aware of the history and application of molecular biology.2. Main Contents(1) Definition of Molecular Biology(2) Brief history of Molecular Biology(3) Application of Molecular Biology3. Arrangement1 class hour (1 teaching hour)4.Teaching MethodsLecture with Multimedia5.Key Points and Difficult PointsKey Points: the central dogmaDifficult Points:genome and proteomics6. LanguageEnglishChapter1DNA Structure,Replication,andRepair1.Objectives &Requirements(1) Through this chapter study, students should know the molecular mechanismof inheritance and the molecular mechanism of some diseases.(2) Understand the structure of DNA.(3)Be familiar with the process of prokaryotic DNA Synthesis, the names andfunctions of proteins and enzymes involved in prokaryotic DNA Synthesis, theprocess of eukaryotic DNA Synthesis, the names and functions of proteins andenzymes involved in eukaryotic DNA Synthesis, the organization of eukaryotic DNA,andthetypesandmechanismsofDNArepair
2 Introduction 1. Objectives & Requirements (1) Understand the definition and the significance of the central dogma. (2) Be familiar with the definition of molecular biology, gene, genome, and proteomics. (3) Be aware of the history and application of molecular biology. 2. Main Contents (1) Definition of Molecular Biology (2) Brief history of Molecular Biology (3) Application of Molecular Biology 3. Arrangement 1 class hour (1 teaching hour) 4. Teaching Methods Lecture with Multimedia 5. Key Points and Difficult Points Key Points: the central dogma Difficult Points:genome and proteomics 6. Language English Chapter 1 DNA Structure, Replication, and Repair 1. Objectives & Requirements (1) Through this chapter study, students should know the molecular mechanism of inheritance and the molecular mechanism of some diseases. (2) Understand the structure of DNA. (3) Be familiar with the process of prokaryotic DNA Synthesis, the names and functions of proteins and enzymes involved in prokaryotic DNA Synthesis, the process of eukaryotic DNA Synthesis, the names and functions of proteins and enzymes involved in eukaryotic DNA Synthesis, the organization of eukaryotic DNA, and the types and mechanisms of DNA repair
(4) Be aware of reverse transcription2. Main Contents(1)Overview(2) Structure of DNA(3) Steps in prokaryotic DNA Synthesis(4) Eukaryotic DNA replication(5)OrganizationofeukaryoticDNA(6) DNA Repair3. Arrangement5 class hours (5 teaching hours)4.Teaching Methods(1) Lecture with Multimedia(2) Group discussion5.KeyPoints and Difficult PointsKey points:(I) The process of prokaryotic DNA Synthesis(2) The names and functions of proteins and enzymes involved in prokaryoticDNA SynthesisDifficult points:()The process of prokaryotic DNA Synthesis(2) The names and functions of proteins and enzymes involved in prokaryoticDNA Synthesis6.LanguageEnglish.Chapter 2 RNA Structure, Synthesis, and Processing1.Objectives &Requirements(l)Students shouldknowthesignificanceofgeneexpression(2) Understand the concepts of transcription, polycistronic, monocistronic,TATA box, holoenzyme, general transcription factor, exon, intron, splicing.m
3 (4) Be aware of reverse transcription. 2. Main Contents (1) Overview (2) Structure of DNA (3) Steps in prokaryotic DNA Synthesis (4) Eukaryotic DNA replication (5) Organization of eukaryotic DNA (6) DNA Repair 3. Arrangement 5 class hours (5 teaching hours) 4. Teaching Methods (1) Lecture with Multimedia (2) Group discussion 5. Key Points and Difficult Points Key points: (1) The process of prokaryotic DNA Synthesis (2) The names and functions of proteins and enzymes involved in prokaryotic DNA Synthesis Difficult points: (1) The process of prokaryotic DNA Synthesis (2) The names and functions of proteins and enzymes involved in prokaryotic DNA Synthesis 6.Language English. Chapter 2 RNA Structure, Synthesis, and Processing 1.Objectives & Requirements (1) Students should know the significance of gene expression. (2) Understand the concepts of transcription, polycistronic, monocistronic, TATA box, holoenzyme, general transcription factor, exon, intron, splicing
(3) Be familiar with RNA Structure, the transcription of Prokaryotic gene, thetranscription of Eukaryotic gene,the characteristics of post-transcriptionalModification of RNA(4)BeawareofthemethodsofRNAmeasurement2.Main Contents(1)Overview of RNA Structure(2) Transcription of Prokaryotic gene(3)Transcription of Eukaryotic gene(4) Post-transcriptional Modification of RNA3.Arrangement3 class hours (3 teaching hours)4.Teaching Methods() Lecture with Multimedia(2) Group discussion5.Key Points and Difficult PointsKey Points:(I) Steps in RNA synthesis(2) Sigma factor(α) and -35 sequence in prokaryotes(3)Promoter for RNA pol II in eukaryotes(4) SplicingDifficult Points:() Functions of p factor and rho-dependent termination(2) Mechanism of splicing6.LanguageEnglishChapter3ProteinSynthesis1.Objectives &Requirements(1) Understand the concepts of translation, genetic code, degeneracy, anticodon,SD sequence, Protein targeting. Understand the covalent attachments duringposttranslational processing4
4 (3) Be familiar with RNA Structure, the transcription of Prokaryotic gene, the transcription of Eukaryotic gene, the characteristics of post-transcriptional Modification of RNA (4) Be aware of the methods of RNA measurement. 2.Main Contents (1) Overview of RNA Structure (2) Transcription of Prokaryotic gene (3) Transcription of Eukaryotic gene (4) Post-transcriptional Modification of RNA 3.Arrangement 3 class hours ( 3 teaching hours) 4.Teaching Methods (1) Lecture with Multimedia (2) Group discussion 5.Key Points and Difficult Points Key Points: (1) Steps in RNA synthesis (2) Sigma factor (σ) and -35 sequence in prokaryotes (3) Promoter for RNA pol II in eukaryotes (4) Splicing Difficult Points: (1) Functions of ρ factor and rho-dependent termination (2) Mechanism of splicing 6.Language English Chapter 3 Protein Synthesis 1.Objectives & Requirements (1) Understand the concepts of translation, genetic code, degeneracy, anticodon, SD sequence, Protein targeting. Understand the covalent attachments during posttranslational processing
(2) Be familiar with the genetic code (61 sense codons, 3 termination codons.),the characteristics of the genetic code, the components required for translation, thewobble hypothesis, the steps in protein synthesis.2.Main Contents(1) Protein Biosynthesis System(2) Activation of Amino Acids(3)Protein Biosynthesis Steps (Initiation, Elongation,Termination)(4) Protein Folding and Posttranslational Processing3.Arrangement6 class hours (6 teaching hours)4.Teaching Methods(1) Lecture with Multimedia(2) Group discussion5.Key Points and Difficult PointsKey Points:1. Steps in protein synthesis2. The three-base codon determined one amino acid.3. Steps in amino-acylationDifficult Points:1. The amino acylation reaction2. Functions of A, P, and E sites on the ribosome3.Polypeptide covalently modification6.LanguageEnglishChapter4RegulationofGeneExpression1.Objectives &Requirements(1) Understand the characteristics of the tryptophan (trp) operon, the concepts oftranscription, housekeeping genes, cis-acting element, trans-acting factor, operon.stringent response, mRNA ed iting, RNA interference.5
5 (2) Be familiar with the genetic code (61 sense codons, 3 termination codons.), the characteristics of the genetic code, the components required for translation, the wobble hypothesis, the steps in protein synthesis. 2.Main Contents (1) Protein Biosynthesis System (2) Activation of Amino Acids (3) Protein Biosynthesis Steps ( Initiation, Elongation,Termination) (4) Protein Folding and Posttranslational Processing 3.Arrangement 6 class hours (6 teaching hours) 4.Teaching Methods (1) Lecture with Multimedia (2) Group discussion 5.Key Points and Difficult Points Key Points: 1. Steps in protein synthesis 2. The three-base codon determined one amino acid. 3. Steps in amino-acylation Difficult Points: 1. The amino acylation reaction 2. Functions of A, P, and E sites on the ribosome 3. Polypeptide covalently modification 6.Language English Chapter 4 Regulation of Gene Expression 1.Objectives & Requirements (1) Understand the characteristics of the tryptophan (trp) operon, the concepts of transcription, housekeeping genes, cis-acting element, trans-acting factor, operon, stringent response, mRNA editing, RNA interference