8 CHAPTER 7 Scale-up of Aerobic Processes
Chapter 7 Scale-up of Aerobic Processes
7.1.Introduction of Scale-up 反应器内的浓 颗粒团聚， 颗粒聚团内 度场、温度场、 细胞内代 基因DNA、 气泡聚并 的菌体细胞 谢网络 RNA网络 速度场分布 ↓ Reactor Cell metabilism Gene express
7.1. Introduction of Scale-up 反应器内的浓 度场、温度场、 速度场分布 颗粒团聚、 气泡聚并 颗粒聚团内 的菌体细胞 细胞内代 谢网络 基因DNA、 RNA网络 Reactor Cell metabilism Gene express
放大过程 基于细胞群体效应和传递特性的直接放大新思路 面向：传统的生物过程逐级放大方法 摇瓶 实验室 中试 发酵罐 生产 6 工业化生产
放大过程 基于细胞群体效应和传递特性的直接放大新思路 摇瓶 实验室 发酵罐 工业化生产 中试 生产 面向：传统的生物过程逐级放大方法
Increasing productivity improving process controllability Integrated bioprocess optimisation Environmental influences Bio-informatics Genomics (DNA sequence) Cell Reactor DSP Transcriptomics (mRNA) Proteomics PRODUCT (proteins) Metabolomics (metabolites) Biocatalyst Integrated bioprocess optimisatlon
Pseudo plastic Newtonian P dv/dy (s1) Rheogram showing the flow behaviour of Newtonian and pseudoplastic media.T slopes of the dotted lines represent the apparent viscosity (a)of the pseudoplastic liquid att different shear rates
Scale up (1)inherent property Gene influecnce metabilism (2)Environmental Mass trasformation Homogenious system
Scale up (1) inherent property Gene influecnce metabilism (2)Environmental Mass trasformation Homogenious system
Side view:Flow pattern: Top view: Disc turbine 十 Open turbine 哥 8网 Propeller 嫩0 Common stirrer types used in bioreactors.Left hand figures illustrate the liquid flow pattern
-Fig.5- 表1常用搅拌器的分类 分英标准 1类 ,共 3 齿靠向升 平叶搅器 表2几种主要搅拌器的规格 名称觉#叶数DTc/D上/D。 6 0.33 Prochem Maxflo T 50.10.420.01230 Lightnin A315 h.100.200.01232 :搅拌器叶片上袭与下缘的连线和水平面的夹角：D: 搅排的直径：1轮的直径T:发屏的直径：x:搅器 叶片空回的轴向厚度 Table 4- Power numbers and flow numbers data at high Reynolds numbers22 Impeller type Power Flow number number (Po)(Fi) Rushton 5.2 9coeaeaa8geaempeier 1.27 0.23 FoyrATr 0.75 0.73 NemnRceaor ced gas dispersion g38a8r0g (four blades);(c Maxfio
Rushton Turbine Prochem Hydrofoil Diagram of Flat-Blade Rushton Turbine Impeller and Prochem Maxflo Hydrofoil Impeller
11⊙LD171© IG [ONE OF TWO) TAPERED THREAD DAPELLER HUB SEGMENT GROCVES r90eagoog89 FOR KEY Composieneod ring holsrse ment hut togother. MOLDEO-IN KEY A6000 Impeller Manufactured from composite material